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2.3.1.183: phosphinothricin acetyltransferase

This is an abbreviated version!
For detailed information about phosphinothricin acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.183

Reaction

acetyl-CoA
+
Phosphinothricin
=
CoA
 +
N-acetylphosphinothricin

Synonyms

BAR, DR_1182, GK0593, GK2920, hpat, L-PPT N-acetyltransferase, MAT, methionine sulfone N-acetyltransferase, Pat, phosphinothricin acetyltransferase, phosphinothricin N-acetyltransferase, phosphinothricin-N-acetyltransferase, PitA, PPT acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.183 phosphinothricin acetyltransferase

Cloned

Cloned on EC 2.3.1.183 - phosphinothricin acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a linear template for in vitro transcription and translation is created, the PAT gene is cloned into the vector pCR2.1TOPO, verified and subcloned into pET280 and pDOW1169 for expression in Escherichia coli or Pseudomonas fluorescens, respectively
-
expressed as chimeric protein in Escherichia coli and in pea chloroplasts after transformation with Agrobacterium tumefaciens
expressed in Escherichia coli and in genetically modified zoysia grass Zoysia matrella
-
expressed in Festuca pratensis after transformation with Agrobacterium tumefaciens
-
expressed in Quercus suber after transformation with Agrobacterium tumefaciens
expressed in soybean after transformation with Agrobacterium tumefaciens
expression in Escherichia coli
expression in Escherichia coli C41(lambdaDE3)
gene bar, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), expression in transgenic maize and rape plant leaves
gene bar, expression in Escherichia coli with gene fragments fused to the 3' end, the fusion gene product shows unaltered kinetic properties compared to the unmodified gene product
-
gene bar, subcloning in Escherichia coli, functional expression in Nicotiana tabacum protoplasts using the Agrobacterium tumefaciens transfection system and The S35 CMV vector
-
gene bar, transformation of and expression in chloroplasts of Nicotiana tabacum cv. Havana, reciprocal crosses between wild type and transplastomic tobacco plants confirms maternal inheritance of the PPT resistance and high levels of PAT activity in the transplastomic plants, phenotype, overview
-
gene pat, DNA and amino acid sequence determination and analysis, translation of pat is initiated by a GTG codon, functional expression in transgenic Nicotiana tabacum plants by Agrobacterium tumefaciens strain LBA4404-mediated leaf-disc transformation replacing the GTG start codon by ATG, expression of wild-type and mutant enzymes in Streptomyces lividans strain TK23 and in Escherichia coli strains JM83 and S17.1, the gene confers resistance to L-phosphinothricin
-
gene pat, expression in Capsicum annuum var. Subicho through Agrobacterium-mediated transformation, expression is highest in leaves, followed by stems, pollen, fruits, and lowest in roots
-
gene pat, expression in Nicotiana tabacum var. W38 plant leaves and roots using the root-specific pat promoter of the hemoglobin gene from Parasponia andersonii, the transgenic tobacco plants show herbicide tolerance, overview
-
gene pat, expression in transgenic Nicotiana tabacum, Daucus carota, and Medicago sativa plants, the functional expression of gene pat confers resistance to the herbicide L-phosphinothricin
-
gene pat, functional expression in transgenic Nicotiana tabacum and Daucus carota plant leaves using the Agrobacterium tumefaciens strain LBA4404 transfection of leaves and direct gene transfer to protoplasts, respectively, subcloning in Escherichia coli strain S 17. I, the transgenic plants are resistant to L-phosphinothricin
-
gene PttR, cloning of the gene from an phosphinothricin-tripeptide-resistant mutant, DNA and amino acid sequence determination and analysis, promoter analysis, expression in Streptomyces lividans strain TK23 and Escherichia coli strain JM83 using different plasmids, overview, the gene confers resistance to L-phosphinothricin
-
into the pQE31 vector for expression in Escherichia coli M15 cells
-
into the vector pBFT, the new construct contains the marker genes for phosphinothricin acetyltransferase and green fluorescence protein as a fusion sequence bar::egfp
-
into the vector pET24a for expression in Escherichia coli BL21DE3 cells
overexpression in Escherichia coli
-
overexpression in Escherichia coli Bl21(DE3) and Pseudomonas fluorescens strain DC454 requiring modification of the 5' end of the pat ORF, in vitro transcription and translation, optimization of the expression method, Pseudomonas fluorescens is the better host, the secondary structures in the 5' coding region of pat gene influences the expression efficiency, overview
-
pat gene is inserted into glufosinate-resistant maize hybrids (Herculex, Agrisure TL, Herculex Yieldgard, Leptra, Viptera 3, Power Core)
-