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2.3.1.189: mycothiol synthase

This is an abbreviated version!
For detailed information about mycothiol synthase, go to the full flat file.

Word Map on EC 2.3.1.189

Reaction

desacetylmycothiol
+
acetyl-CoA
=
CoA
+
mycothiol

Synonyms

MSH acetyltransferase, MSH synthase, MshD, nshD, Rv0819

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.189 mycothiol synthase

Cloned

Cloned on EC 2.3.1.189 - mycothiol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in pQE31 plasmid and transformed into Escherichia coli M15 (pREP4)
expression in Escherichia coli
the Rv0819 gene encoding MshD (residues 1–315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS