2.3.1.193: tRNAMet cytidine acetyltransferase
This is an abbreviated version!
For detailed information about tRNAMet cytidine acetyltransferase, go to the full flat file.
Reaction
Synonyms
tmcA, ypfI
ECTree
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Substrates Products
Substrates Products on EC 2.3.1.193 - tRNAMet cytidine acetyltransferase
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REACTION DIAGRAM
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
[elongator tRNAMet]-cytidine34 + GTP + acetyl-CoA
[elongator tRNAMet]-N4-acetylcytidine34 + GDP + phosphate + coenzyme A
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[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
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tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop
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[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
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ATP/GTP hydrolysis by TmcA is stimulated in the presence of acetyl-CoA and tRNAMet. TmcA hydrolyses ATP or GTP between the beta- and gamma-phosphates, as shown by the detection of labelled ADP (or GDP). A mutation study reveals that Escherichia coli TmcA strictly discriminates elongator tRNAMet from the structurally similar tRNAIle by mainly recognizing the C27G43 pair in the anticodon stem
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