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2.3.1.212: benzalacetone synthase

This is an abbreviated version!
For detailed information about benzalacetone synthase, go to the full flat file.

Word Map on EC 2.3.1.212

Reaction

4-coumaroyl-CoA
+
malonyl-CoA
+
H2O
= 2 CoA +
4-hydroxybenzalacetone
+ 2 CO2

Synonyms

bAS, HsPKS3, PKS1, RinPKS1, RiPKS4, RpBAS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.212 benzalacetone synthase

Cloned

Cloned on EC 2.3.1.212 - benzalacetone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged wild-type and mutant enzymes in Escherichia coli
expressed in Escherichia coli Rosetta-gami (DE3) and BL21-Rosetta (DE3) cells
expression in Escherichia coli
overexpressed in Escherichia coli
overexpressed in Escherichia coli as a C-terminally poly-His-tagged fusion protein
-
PKS genotyping, gene products RiPKS5, RiPKS6 and RiPKS11 show negligible BAS activity
-
RiPKS4, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLYsS
sequence comparison