2.3.1.229: 4-coumaroyl-homoserine lactone synthase
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For detailed information about 4-coumaroyl-homoserine lactone synthase, go to the full flat file.
Reaction
Synonyms
acyl-homoserine lactone synthase, acyl-HSL synthase, autoinducer (acylhomoserine lactone) synthase, p-coumaryl-homoserine lactone synthase, pcoumaroyl-HSL synthase, RpaI
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Application
Application on EC 2.3.1.229 - 4-coumaroyl-homoserine lactone synthase
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synthesis
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development of an artificial biosynthetic process for phenylacetyl-homoserine lactone analogs, including cinnamoyl-homoserine lactone, 4-coumaroyl-homoserine lactone, caffeoyl-homoserine lactone, and feruloyl-homoserine lactone, using coexpression of the codon-optimized synthase RpaI and 4-coumaroyl-CoA ligase 4CL2nt, EC 6.2.1.12, in Escherichia coli. De novo production of p-coumaroyl-homoserine lactone in Escherichia coli can be achieved by expression of the rpaI gene in addition to 4-coumaroyl-CoA biosynthetic genes. The yields for 4-coumaroyl-homoserine lactone reach 93 and 142 mg/l in the S-adenosyl-L-methionine and L-methionine feeding culture, respectively
synthesis
production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway. An Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-homoserine lactones from simple carbon sources. The artificial biosynthetic pathways contain the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. The yields for caffeoyl-phenylacetyl-homoserine lactone and feruloyl-phenylacetyl-homoserine lactone are 97.1 and 65.2 mg/l, respectively, by tyrosine-overproducing Escherichia coli with a L-methionine feeding strategy
synthesis
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production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway. An Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-homoserine lactones from simple carbon sources. The artificial biosynthetic pathways contain the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. The yields for caffeoyl-phenylacetyl-homoserine lactone and feruloyl-phenylacetyl-homoserine lactone are 97.1 and 65.2 mg/l, respectively, by tyrosine-overproducing Escherichia coli with a L-methionine feeding strategy
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