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2.3.1.239: 10-deoxymethynolide synthase

This is an abbreviated version!
For detailed information about 10-deoxymethynolide synthase, go to the full flat file.

Word Map on EC 2.3.1.239

Reaction

malonyl-CoA
+ 5 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ =
10-deoxymethynolide
+ 6 CoA + 6 CO2 + 5 NADP+ + 2 H2O

Synonyms

methymycin/picromycin polyketide synthase, picromycin/methymycin PKS, picromycin/methymycin polyketide synthase, PICS, PikAI, PikAIII, PikAIV, pikromycin PKS, pikromycin polyketide synthase, type I polyketide synthase PikAIV

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.239 10-deoxymethynolide synthase

Cloned

Cloned on EC 2.3.1.239 - 10-deoxymethynolide synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Streptomyces venezuelae
-
expression of functional polyketide synthase modules in Escherichia coli
overexpression of pikromycin modules PikAIII and PIkAIV in Escherichia coli
the picromycin/methymycin PKS genes (pikAI, PikAII, PikAIII, PikAIV and pikAV) are functionally expressed in the heterologous host Streptomyces lividans, resulting in production of both narbonolide and 10-deoxymethynolide
the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli
-