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2.3.1.240: narbonolide synthase

This is an abbreviated version!
For detailed information about narbonolide synthase, go to the full flat file.

Reaction

malonyl-CoA
+ 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ =
narbonolide
 + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O

Synonyms

methymycin/picromycin polyketide synthase, picromycin/methymycin PKS, picromycin/methymycin polyketide synthase, PICS, PikAI, PikAIII, PikAIV, pikromycin PKS, pikromycin polyketide synthase, type I polyketide synthase PikAIV

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.240 narbonolide synthase

Cloned

Cloned on EC 2.3.1.240 - narbonolide synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Streptomyces venezuelae
-
expression of functional polyketide synthase modules in Escherichia coli
overexpression of pikromycin modules PikAIII and PIkAIV in Escherichia coli
overexpression of polyketide synthase PikAIII, and polyketide synthase PikAIV in Escherichia coli
the picromycin/methymycin PKS genes (pikAI, PikAII, PikAIII, PikAIV and pikAV) are functionally expressed in the heterologous host Streptomyces lividans, resulting in production of both narbonolide and 10-deoxymethynolide
the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli