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2.3.1.26: sterol O-acyltransferase

This is an abbreviated version!
For detailed information about sterol O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.26

Reaction

a long-chain acyl-CoA
+
a sterol
=
CoA
+
a long-chain 3-hydroxysterol ester

Synonyms

A2, ACAT, ACAT-1, ACAT-2, ACAT1, ACAT2, acyl coenzyme A-cholesterol-O-acyltransferase, acyl coenzyme A: cholesteryl acyltransferase, acyl-CoA cholesterol acyltransferase, acyl-CoA cholesterol O-acyltransferase, acyl-CoA: cholesterol acyltransferase, acyl-CoA:cholesterol acyltransferase, acyl-CoA:cholesterol acyltransferase 1, acyl-CoA:cholesterol acyltransferase 2, acyl-coA:cholesterol o-acyl transferase 2, acyl-coenzyme A: cholesterol acyltransferase, acyl-coenzyme A:cholesterol acyltransferase, acyl-coenzyme A:cholesterol acyltransferase 1, acyl-coenzyme A:cholesterol acyltransferase 2, acyl-coenzyme A:cholesterol acyltransferase-1, acyl-coenzyme A:cholesterol O-acyltransferase, acyl-coenzymeA cholesterol acyltransferase, acyl-coenzymeA:cholesterol acyl-transferase, acylcoenzyme A:cholesterol O-acyltransferase, acyltransferase, cholesterol, AsAT, cholesterol acyltransferase, cholesterol acyltransferase 2, cholesterol ester synthase, cholesterol ester synthetase, cholesteryl ester synthetase, hACAT, hACAT-1, hACAT-2, hACAT1, SOAT, SOAT!, Soat1, Soat2, sterol O-acyltransferase 2, sterol-ester synthase, sterol-ester synthetase, steroyl O-acyltransferase 1, steroyl O-acyltransferase 2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.26 sterol O-acyltransferase

Cloned

Cloned on EC 2.3.1.26 - sterol O-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant baculovirus transfer vector is constructed by subcloning of human ACAT-1 cDNA into pFASTBacHis
-
a recombinant baculovirus transfer vector is constructed by subcloning of human ACAT-2 cDNA into pFASTBacHis
-
a stable cell line expressing hACAT-1 from AC-29 cells is established
-
a stable cell line expressing hACAT-2 from AC-29 cells is established
-
all constructs, full-length, chimeras and mutants are cloned into the pCDNA3 vector, AC29 cells are transfected
-
cDNA cloned
CHO cells stably overexpressing human ACAT-1 are used
-
CHO cells stably overexpressing human ACAT-2 are used
-
expressed as a His-tagged fusion protein in Escherichia coli
expression in AC29 cells
-
expression in CHO cell
expression in CHO cells
expression in enzyme-deficient mutant chinese hamster ovary cells
expression in HEK 293 cell
expression in human embryonic kidney A293 cells and expression in Chinese hamster ovary cells
-
expression in insect SF9 cells
-
expression in sat1 sat2 mutant cells of Saccharomyces cerevisiae
-
expression in Xenopus oocytes
-
expression of ACAT1 in infected insect High Five cells
-
expression of an antisense cDNA in HepG2 cells
-
expression of isoforms ACAT1 and ACAT2 in CHO cell
expression of isoforms ACAT1 and ACAT2 in CHO cells
expression of isoforms ACT1 and ACAT2 in CHO cell
gene ACAT1, human Acat1 is located in two different chromosomes, chromosomes 1 and 7, with each site containing a distinct promoter: chromosome 1 contains exons 1-16, and chromosome 7 contains the optional long exon Xa, recombinant expression, functional complementation of a Chinese hamster ovary cell mutant lacking ACAT activity, the majority of ACAT1 mRNAs is transcribed from exons 1-16
gene ACAT1, in mouse the Acat1 gene is located solely on chromosome 1 and contains 17 exons
gene ACAT2, genotying, sequence comparison and phylogenetic analysis, two human ACAT2 gene polymorphisms have significant effects on 41A-G (Glu14Gly, rs9658625) and 734C-T (Thr254Ile, rs2272296), on plasma lipid levels and coronary artery disease susceptibility, recombinant expression of wild-tpe and mutant enzymes in ACAT-deficient AC-29 cells
gene ACAT2, human gene Acat2 is located in chromosome 12
gene Soat1, quantitative real-time PCR expression analysis
into the PCDNA3 vector
mutants generated by baculovirus expression technology
presence of two gene products: ACAT1 and ACAT2, the gene product ACAT2 cloned from an African green monkey liver cDNA library. Transfection of the cloned ACAT2 cDNA into two different mammalian cell types
-
production of glutathione S-transferase-ACAT1 fusion proteins, expression of fusion proteins in Escherichia coli and expression of various recombinant HisACAT1s in infected H5 cells
-
recombinant hACAT1 (HisACAT1/Flag) is expressed in HEK293 cells
-
recombinant hACAT1 expressed in CHO cells
sequence analysis and human cDNA clones indicate homology to liver carboxylesterase
-
the baculovirus system is used
two genes, SAT1 and SAT2 encode isozymes of the enzyme
using the baculovirus system
-