2.3.1.30: serine O-acetyltransferase
This is an abbreviated version!
For detailed information about serine O-acetyltransferase, go to the full flat file.
Word Map on EC 2.3.1.30
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2.3.1.30
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o-acetylserine
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sulfur
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l-cysteine
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sulfhydrylase
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o-acetyl-l-serine
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foot-and-mouth
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desulfhydrase
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oas-tls
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o-acetylserinethiollyase
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assimilatory
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slc26a1
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nasi-1
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thlaspi
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bienzyme
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phytochelatins
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molecular biology
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agriculture
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drug development
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medicine
- 2.3.1.30
- o-acetylserine
- sulfur
- l-cysteine
-
sulfhydrylase
- o-acetyl-l-serine
- foot-and-mouth
- desulfhydrase
- oas-tls
-
o-acetylserinethiollyase
-
assimilatory
-
slc26a1
-
nasi-1
- thlaspi
-
bienzyme
- phytochelatins
- molecular biology
- agriculture
- drug development
- medicine
Reaction
Synonyms
acetyltransferase, serine, AtOASS, AtSAT, AtSAT1, AtSerat 2;1, BvSAT, CysE, cytosolic serine acetyltransferase, DcsE, EhSAT1, EhSAT2, EhSAT3, GmSerat2,1, HISAT, Kpn CysE, L-serine acetyltransferase, L-serine O-acetyltransferase, L-serineO-acetyltransferase, More, plastidic serine acetyltransferase, SAT, SAT isoform 1, SAT-1, SAT1, SAT2, SAT3, SATase, SERAT, Serat1,1, SERAT1.1, Serat2,1, Serat2,2, SERAT2.1, Serat3,1, Serat3,2, serine acetlytransferase, serine acetyltransferase, serine acetyltransferase 1, serine acetyltransferase 2,1, serine O-acetyltransferase, serine O-acetyltransferase 1, serine transacetylase, Spn cysE, SP_0589
ECTree
2.3.1.30 serine O-acetyltransferaseAdvanced search results
results (
results (Specific Activity
Specific Activity on EC 2.3.1.30 - serine O-acetyltransferase
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.00036
purified recombinant mitochondrial isozyme A, mutant H327A
0.00134
purified recombinant mitochondrial isozyme A, mutant V353E
0.00389
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substrate L-serine, pH 7.5, 37°C, recombinant wild-type enzyme
0.00513
purified recombinant mitochondrial isozyme A, mutant G354A
0.032
purified recombinant mitochondrial isozyme A, wild-type
176.8
Vmax with acetyl-CoA, 50 mM Tris-HCl, pH 8.0, 1 mM L-serine, 25°C
23.42
Vmax with acetyl-CoA, 50 mM Tris-HCl, pH 8.0, 1 mM L-serine, 25°C
25
recombinant enzyme, isozyme VvSERAT2-2 in the cysteine synthase complex, pH and temperature not specified in the publication
27.77
Vmax with L-serine, 50 mM Tris-HCl, pH 8.0, 0.1 mM acetyl-CoA, 25°C
41.39
Vmax with L-serine, 50 mM Tris-HCl, pH 8.0, 0.1 mM acetyl-CoA, 25°C
65.75
Vmax with L-serine, 50 mM Tris-HCl, pH 8.0, 0.1 mM acetyl-CoA, 25°C
95.1
Vmax with acetyl-CoA, 50 mM Tris-HCl, pH 8.0, 1 mM L-serine, 25°C
additional information
additional information
50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots
additional information
50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots
additional information
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50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots
additional information
50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves
additional information
50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves
additional information
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50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves
additional information
50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots
additional information
50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots
additional information
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50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots
additional information
recombinant Arabidopsis serine acetlytransferase activity in transgenic Lupinus angustifolius embryos of the T2 and T4 plant generation is increased severalfold over controls (wild-type and transgenic sunflower seed albumin lupins), 5-12fold and about 5fold in 5F1 (in vitro 4fold over wild-type), 24-31fold and about 20fold in 3A3 (in vitro 12fold over wild-type), 49fold and about 40fold in 5B3 (in vitro 30fold over wild-type), control enzyme activity is roughly about 5 nmol/min/mg protein, correlation to the concentration of key intermediates of the sulphur amino acid biosynthesis (O-acetylserine, free cysteine, glutathione)
additional information
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recombinant Arabidopsis serine acetlytransferase activity in transgenic Lupinus angustifolius embryos of the T2 and T4 plant generation is increased severalfold over controls (wild-type and transgenic sunflower seed albumin lupins), 5-12fold and about 5fold in 5F1 (in vitro 4fold over wild-type), 24-31fold and about 20fold in 3A3 (in vitro 12fold over wild-type), 49fold and about 40fold in 5B3 (in vitro 30fold over wild-type), control enzyme activity is roughly about 5 nmol/min/mg protein, correlation to the concentration of key intermediates of the sulphur amino acid biosynthesis (O-acetylserine, free cysteine, glutathione)
additional information
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coupling reaction with cysteine synthase, 50 mM Tris-HCl, pH 8.0, 0.4 mM acetyl-CoA, 4 mM L-serine, 5 mM Na2S, 10 mM DTT, recombinant Entamoeba histolytica cysteine synthase 3, 37°C (amount of L-cysteine produced)
additional information
coupling reaction with cysteine synthase, 50 mM Tris-HCl, pH 8.0, 0.4 mM acetyl-CoA, 4 mM L-serine, 5 mM Na2S, 10 mM DTT, recombinant Entamoeba histolytica cysteine synthase 3, 37°C (amount of L-cysteine produced)
additional information
coupling reaction with cysteine synthase, 50 mM Tris-HCl, pH 8.0, 0.4 mM acetyl-CoA, 4 mM L-serine, 5 mM Na2S, 10 mM DTT, recombinant Entamoeba histolytica cysteine synthase 3, 37°C (amount of L-cysteine produced)
additional information
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at acetyl-CoA concentration 15fold higher than L-serine concentration, a nonproductive ternary complex of enzyme-CoA-L-serine is formed, kinetics
