2.3.1.32: lysine N-acetyltransferase
This is an abbreviated version!
For detailed information about lysine N-acetyltransferase, go to the full flat file.
Word Map on EC 2.3.1.32
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2.3.1.32
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coactivator
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chromatin
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transactivation
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e1a
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creb-binding
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gnat
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adenovirus
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oncoproteins
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deacetylases
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hdacs
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nucleosomal
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creb
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hypoxia-inducible
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deacetylation
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element-binding
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bromodomains
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p300-mediated
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smads
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hyperacetylation
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acetylase
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trichostatin
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h3k27ac
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corepressors
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non-histone
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p300-dependent
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accoa
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p53-dependent
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myod
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p53-mediated
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tax
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htlv-1
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medicine
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brd4
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pharmacology
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tata-binding
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selangor
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h3k4me1
- 2.3.1.32
-
coactivator
- chromatin
-
transactivation
- e1a
-
creb-binding
-
gnat
- adenovirus
- oncoproteins
- deacetylases
- hdacs
-
nucleosomal
- creb
-
hypoxia-inducible
-
deacetylation
-
element-binding
-
bromodomains
-
p300-mediated
- smads
-
hyperacetylation
-
acetylase
-
trichostatin
-
h3k27ac
-
corepressors
-
non-histone
-
p300-dependent
- accoa
-
p53-dependent
- myod
-
p53-mediated
- tax
- htlv-1
- medicine
- brd4
- pharmacology
-
tata-binding
-
selangor
-
h3k4me1
Reaction
Synonyms
acetyltransferase p300, acetyltransferase, lysine, ATase1, ATase2, Cbp, cyclic adenosine monophosphate response element-binding binding protein, Esa1, Gcn5, GCN5-A, GCN5-related N-acetyltransferase, GNAT, H4 lysine acetyltransferase, HAT, histone/protein lysine acetyltransferase, KAT, lysine acetyltransferase, NuA4, p/CAF, p300, p300 acetyltransferase, p300/CBP, PCAF, Rtt109 histone acetyltransferase, SePat
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Purification
Purification on EC 2.3.1.32 - lysine N-acetyltransferase
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cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation
cells are washed with PBS, harvested in Tris-HCl, pH 7.4, containing 0.5% deoxycholic acid, 150 mM NaCl, 0.1% SDS, 4 mM EDTA, and 1% NP-40, with a protease inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, centrifugation, supernatant subjected to SDS-PAGE, or for immunoprecipitation lysed cells are collected with 50 mM Tris-HCl, pH 8.0, with 10% glycerol, 100 mM NaCl, 1 mM EDTA, and 0.1% NP-40 with proteinase inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, incubation with antibodies and G beads, washing, elution of proteins by boiling in 2X Laemmli loading buffer
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