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2.3.1.37: 5-aminolevulinate synthase

This is an abbreviated version!
For detailed information about 5-aminolevulinate synthase, go to the full flat file.

Word Map on EC 2.3.1.37

Reaction

succinyl-CoA
+
glycine
=
5-aminolevulinate
 +
CoA
 +
CO2

Synonyms

5-ALA synthase, 5-ALAS, 5-aminolevulinate synthase, 5-aminolevulinate synthetase, 5-aminolevulinic acid synthase, 5-aminolevulinic acid synthetase, ALA, ALA synthase, ALA synthetase, ALA-S, ALAS, ALAS2, alpha-aminolevulinic acid synthase, aminolevulinate synthase, aminolevulinate synthase 2, aminolevulinate synthetase, aminolevulinic acid synthase, aminolevulinic acid synthase 2, aminolevulinic acid synthetase, aminolevulinic synthetase, delta-ALAS, delta-aminolevulinate synthase, delta-aminolevulinate synthetase, delta-aminolevulinic acid synthase, delta-aminolevulinic acid synthase 2, delta-aminolevulinic acid synthetase, delta-aminolevulinic synthetase, erythroid 5-aminolevulinate synthase, erythroid-specific 5-aminolevulinate synthase, Hem1, hemA, HemT, mALAS-2, sigma-aminolevulinate synthase, synthase, aminolevulinate, synthetase, aminolevulinate

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.37 5-aminolevulinate synthase

Purification

Purification on EC 2.3.1.37 - 5-aminolevulinate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms from low-iron medium
-
2 forms: a complex form and a stripped form that represents a catalytically active protein component of the complex form
-
2 isoenzymes: A and B
-
activator protein
-
difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation
-
erythroid-specific isoform from reticulocytes, papain digestion method
-
high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically
-
His-Trap column chromatography
-
HisTrap column chromatography and Mono Q column chromatography
isoforms I and II
-
Ni superflow resin column chromatography
-
Ni2+-NTA agarose resin column chromatography and Superdex S200 gel filtration
nickel affinity chromatography column chromatography
partial
partial purification, 2 isozymes
-
partial, fetal enzyme
-
recombinant from E. coli
-
recombinant MBP-fusion wild-type and mutant enzymes 29-114fold by amylose affinity chromatography and gel filtration
recombinant of His-tagged wild-type and mutants from E. coli
-
recombinant wild-type and Lys313 mutants from Escherichia coli
-
recombinant wild-type and mutant enzymes
recombinant wild-type and mutants from E. coli
-