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2.3.1.41: beta-ketoacyl-[acyl-carrier-protein] synthase I

This is an abbreviated version!
For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase I, go to the full flat file.

Word Map on EC 2.3.1.41

Reaction

an acyl-[acyl-carrier protein]
+
a malonyl-[acyl-carrier protein]
=
a 3-oxoacyl-[acyl-carrier protein]
+
CO2
+
an [acyl-carrier protein]

Synonyms

3-ketoacyl-ACP synthase, 3-ketoacyl-acyl carrier protein synthase, 3-ketoacyl-acyl-carrier protein synthase I, 3-oxoacyl synthase, 3-oxoacyl-[acyl-carrier-protein] synthase, acyl-malonyl(acyl-carrier-protein)-condensing enzyme, B-ketoacyl-ACP synthase I, beta-ketoacyl ACP synthase I, beta-ketoacyl acyl carrier protein synthase, beta-ketoacyl acyl carrier protein synthase I, beta-ketoacyl synthase, beta-ketoacyl synthetase, beta-ketoacyl [ACP] synthase, beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthetase, beta-ketoacyl-ACP-synthase, beta-ketoacyl-ACP-synthase I, beta-ketoacyl-acyl carrier protein synthase I, beta-ketoacyl-acyl carrier protein synthetase, beta-ketoacyl-acyl-ACP synthase I, beta-ketoacyl-CoA synthase-I, beta-ketoacyl-[acyl carrier protein (ACP)] synthase III, beta-ketoacyl-[acyl carrier protein] synthase, beta-ketoacyl-[acyl carrier protein] synthase I, beta-ketoacyl-[acyl-carrier-protein] synthase I, beta-ketoacylsynthase, condensing enzyme, Cys-His-His-type beta-ketoacyl-acyl carrier protein synthase, DpsC, elongase, elongating beta-ketoacyl-acyl carrier protein synthase, FabB, FabBF, FabH, fatty acid condensing enzyme, KAS, Kas A, KAS I, KasA, KasI, KASI-1, KASI-2, KCSI, More, OsKASI, synthase, 3-oxoacyl-[acyl-carrier-protein]

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.41 beta-ketoacyl-[acyl-carrier-protein] synthase I

Purification

Purification on EC 2.3.1.41 - beta-ketoacyl-[acyl-carrier-protein] synthase I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 proteins alpha2, alpha,beta and beta2
-
immobilized metal ion affinity chromatography
immobilized metal ion affinity chromatography (Ni2+)
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography
-
isolation of hexanoyl-enzyme intermediate complex
-
native enzyme from kernel microsomes, solubilization and separation from KAS II by treatment with Triton X-100 and anion exchange chromatography
-
native fatty acid synthase from liver in several steps including gel filtration as the last step
-
Ni-NTA affinity column chromatography and gel filtration
Ni-NTA resin column chromatography
partial
recombinant enzyme
recombinant from E. coli as His-tagged protein
-
recombinant His-tagged enzyme from Escherichia coli strains XL-1 blue and M15
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant His-tagged protein from E. coli
-
recombinant His-tagged proteins FabH1 and FabH2 from E. coli
-
recombinant His-tagged seed-specific isoform from E. coli
-
recombinant His-tagged synthase III from E. coli
recombinant His-tagged synthase IV from E. coli
-
recombinant soluble NusA-fusion protein FabBF from Escherichia coli strain BL21(DE3) by and gel filtration
-
recombinant synthase I and mutants as His-tagged protein from E. coli
-
recombinant synthases I, i.e. FabA, II, i.e. FabB, and III, i.e.FabH
-
synthase II, recombinant from E. coli
-
synthase III, native wild-type and recombinant from E. coli E103S
-
synthase III-like polyketid synthase recombinant from Streptomyces lividans
-
synthases I and II
wild-type and mutants
-