2.3.1.42: glycerone-phosphate O-acyltransferase
This is an abbreviated version!
For detailed information about glycerone-phosphate O-acyltransferase, go to the full flat file.
Reaction
+
=
+
Synonyms
acyltransferase, dihydroxyacetone phosphate, DAP-AT, DAT, DHAP acyltransferase, DHAP-AT, DHAPAT, dihydroxyacetone phosphate acyl-transferase, dihydroxyacetone phosphate acyltransferase, dihydroxyacetone-phosphate acyltransferase, dihydroxyacetonephosphate acyltransferase, glyceronephosphate O-acyltransferase, GNPAT, LmDAT, male sterility protein, TtFARAT
ECTree
Inhibitors
Inhibitors on EC 2.3.1.42 - glycerone-phosphate O-acyltransferase
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5,5'-dithio-bis(2-nitrobenzoate)
acetaldehyde
-
above 0.01 M, microsomal enzyme, above 0.2 M, solubilized peroxisomal enzyme
bovine serum albumin
-
weak
-
Ca2+
-
in crude enzyme extract
chymotrypsin
-
intact vesicles
-
Mercuridextran
-
intact microsomes
-
Mg2+
-
in crude enzyme extract
Mn2+
-
in crude enzyme extract
p-chloromercuriphenylsulfonic acid
phosphatidylethanolamine
-
above 1.5 mM
phosphatidylserine
-
above 1.5 mM
Tris-maleate buffer
-
pH 5.0-6.0
5,5'-dithio-bis(2-nitrobenzoate)
-
not (10 mM)
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB
5,5'-dithio-bis(2-nitrobenzoate)
-
5 mM, moderate; i.e. DTNB
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB; no inhibition
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB; no inhibition
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB; no inhibition
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB; no inhibition
5,5'-dithio-bis(2-nitrobenzoate)
-
i.e. DTNB; no inhibition
cholate
-
-
deoxycholate
-
-
deoxycholate
-
weak, high concentration
deoxycholate
-
above 0.2 mg/ml
deoxycholate
-
inactivation
glycerol 3-phosphate
-
weak
glycerol 3-phosphate
-
pH 6.0-7.0
glycerol 3-phosphate
-
not
glycerol 3-phosphate
-
not
glycerol 3-phosphate
-
adipocyte
glycerol 3-phosphate
-
weak
glycerol 3-phosphate
-
tissue distribution
glycerol 3-phosphate
-
strong, extraperoxisomal activities
glycerol 3-phosphate
-
competitive to dihydroxyacetone phosphate
iodoacetamide
-
5 mM, weak
N-ethylmaleimide
-
no inhibition; not peroxisomal enzyme; weak
N-ethylmaleimide
-
not peroxisomal enzyme
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
not peroxisomal enzyme
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
not peroxisomal enzyme
N-ethylmaleimide
-
no inhibition; not peroxisomal enzyme; weak
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
microsomal enzyme: strong
N-ethylmaleimide
-
mitochondrial enzyme: weak; not peroxisomal enzyme
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
not peroxisomal enzyme
N-ethylmaleimide
-
no inhibition
p-chloromercuriphenylsulfonic acid
-
5 mM
p-chloromercuriphenylsulfonic acid
-
moderate
palmitoyl-CoA
-
weak, above 0.6 mM
palmitoyl-CoA
-
above 0.08 mM; bovine serum albumin protects
palmitoyl-CoA
-
bovine serum albumin protects
Triton X-100
-
-
Triton X-100
-
0.01% w/v; above 0.025% w/v
Triton X-100
-
stimulation up to 0.2 mg/ml; weak
Triton X-100
-
above 0.2 mg/ml
Triton X-100
-
0.1 mg/ml; above 0.1 mg/ml
Trypsin
-
-
-
Trypsin
-
intact vesicles
-
Trypsin
-
not sensitive against
-