2.3.1.43: phosphatidylcholine-sterol O-acyltransferase
This is an abbreviated version!
For detailed information about phosphatidylcholine-sterol O-acyltransferase, go to the full flat file.
Word Map on EC 2.3.1.43
-
2.3.1.43
-
lipoprotein
-
hdl
-
apolipoproteins
-
cholesteryl
-
high-density
-
triglyceride
-
esterification
-
apoa-i
-
atherosclerosis
-
lipase
-
esterify
-
low-density
-
coronary
-
cardiovascular
-
hdl-cholesterol
-
unesterified
-
opacity
-
apoproteins
-
hypercholesterolemia
-
corneal
-
discoidal
-
atherogenic
-
subfractions
-
hdl-associated
-
triglyceride-rich
-
apoc-iii
-
postheparin
-
synthesis
-
hyperlipoproteinemia
-
lysolecithin
-
vldl-c
-
tg-rich
-
dimyristoyl
-
antiatherogenic
-
b-containing
-
normolipidemic
-
cholesterol-loaded
-
xanthoma
-
medicine
-
lipoprotein-cholesterol
-
chylomicron
-
acyl-coa:cholesterol
-
lipid-free
-
paraoxonase
-
apob-containing
-
hdl-mediated
-
3hcholesterol
-
drug development
-
lipid-poor
-
hypertriglyceridemia
-
tangier
-
very-low-density
-
non-hdl
- 2.3.1.43
- lipoprotein
- hdl
-
apolipoproteins
-
cholesteryl
-
high-density
- triglyceride
- esterification
- apoa-i
- atherosclerosis
- lipase
-
esterify
-
low-density
- coronary
- cardiovascular
-
hdl-cholesterol
-
unesterified
- opacity
- apoproteins
- hypercholesterolemia
- corneal
-
discoidal
-
atherogenic
-
subfractions
-
hdl-associated
-
triglyceride-rich
- apoc-iii
-
postheparin
- synthesis
- hyperlipoproteinemia
- lysolecithin
-
vldl-c
-
tg-rich
-
dimyristoyl
-
antiatherogenic
-
b-containing
-
normolipidemic
-
cholesterol-loaded
-
xanthoma
- medicine
-
lipoprotein-cholesterol
- chylomicron
-
acyl-coa:cholesterol
-
lipid-free
- paraoxonase
-
apob-containing
-
hdl-mediated
-
3hcholesterol
- drug development
-
lipid-poor
- hypertriglyceridemia
- tangier
-
very-low-density
-
non-hdl
Reaction
Synonyms
acyltransferase, lecithin-cholesterol, cholesterol transacyltransferase, LAT, LCAT, lecithin cholesterol acyl transferase, lecithin cholesterol acyltransferase, lecithin-cholesterol acyl transferase, lecithin-cholesterol acyltransferase, lecithin/cholesterol acyltransferase, lecithin: cholesterol acyltransferase, lecithin:cholesterol acyl-transferase, lecithin:cholesterol acyltransferase, lysolecithin acyltransferase, phospholipid-cholesterol acyltransferase, plasma lecithin-cholesterol acyltransferase, TgLCAT, TGME49_272420
ECTree
2.3.1.43 phosphatidylcholine-sterol O-acyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.3.1.43 - phosphatidylcholine-sterol O-acyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-bis-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine + cholesterol
cholesteryl-1-pyrenebutyrate + lysophosphatidylcholine
1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine + cholesterol
?
fluorescent substrate assay
-
-
?
1-acylglyceryl phosphorylcholine + lecithin
lecithin + 1-acylglyceryl phosphorylcholine
-
transfer of an acyl group from a lecithin molecule to another on the low-density lipoprotein surface, lysolecithin acyltransferase activity
-
r
1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine + 1-O-acyl-2-lyso-sn-glycerol-3-phosphocholine
1-O-alkyl-2-lyso-sn-glycerol-3-phosphocholine + 1-O-acyl-2-acetyl-sn-glycerol-3-phosphocholine
-
-
-
?
1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O
1-O-alkyl-2-lyso-sn-glycerol-3-phosphocholine + acetate
1-palmitoyl-2-(6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl)-sn-glycero-3-phosphocholine + cerebrosterol
1-palmitoyl-sn-glycero-3-phosphocholine + cerebrosteryl-(6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl)-ester
i.e. 16:0-6:0 NBD-PC + (24S)-hydroxycholesterol
-
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
1-palmitoyl-sn-glycero-3-phosphocholine + cholesteryl oleate
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + dehydroergosterol
1-palmitoyl-glycerophosphocholine + dehydroergosterol 3-O-oleoyl ester
dehydroergosterol is a naturally occurring fluorescent sterol, that is esterified by enzyme LCAT, although at a slower rate than esterification of cholesterol, assay method development, overview
-
-
?
2-sn-phosphorylcholinediacylglycerol + cholesterol
?
-
lower activity than the natural substrate
-
?
cholesterol + 1-O-hexadecyl-2-oleylphosphatidylcholine
cholesteryl oleate + 1-O-hexadecylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-(5Z,8Z,11Z,14Z,17Z)-eicosapenta-5,8,11,14,17-enoylphosphatidylcholine
(3beta)-cholest-5-en-3-yl (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-arachidonoylphosphatidylcholine
cholesteryl arachidonate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-docosahexaenoylphosphatidylcholine
cholesteryl docosahexaenoate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-linoleoylphosphatidylcholine
cholesteryl linoleate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine
cholesteryl oleate + 1-palmitoylglycerophosphocholine
cholesterol + 1-palmitoyl-2-phytanoylphosphatidylcholine
cholesteryl phytanoate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-phytanoyl-2-palmitoylphosphatidylcholine
cholesteryl palmitate + 1-phytanylglyerophosphocholine
-
-
-
?
dioleoyl-phosphatidyl choline + cholesterol
1-oleoyl-phosphatidyl choline + cholesteryl oleate
-
-
-
-
?
L-alpha-phosphatidylcholine type XVI-E + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
p-nitrophenol butyrate + H2O
p-nitrophenol + butyric acid
-
esterase activity
-
?
phosphatidylcholine + 24-hydroxycholesterol
1-acylglycerophosphocholine + a 24-hydroxycholesterol 3-O-acyl ester
-
-
-
?
phosphatidylcholine + cholesterol
3-acylglycerophosphocholine + ?
-
isozyme abnormality cause hepatosplenic schistosomiasis mansoni, important in lipoprotein metabolism and cholesterol transport, cholesterol is trapped in the HDL particles, enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I
-
-
?
phosphatidylcholine + cholesterol
lysolecithin + cholesteryl ester
-
enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I
-
-
?
phosphatidylcholine + cholesterol
lysophosphatidylcholine + cholesteryl ester
-
enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I
-
-
?
phosphatidylcholine + sitosterol
1-acylglycerophosphocholine + sitosteryl ester
-
purified recombinant enzyme
-
-
?
1,2-bis-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine + cholesterol
cholesteryl-1-pyrenebutyrate + lysophosphatidylcholine
-
-
-
?
1,2-bis-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine + cholesterol
cholesteryl-1-pyrenebutyrate + lysophosphatidylcholine
-
activity assay method based on this reaction
-
?
1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O
1-O-alkyl-2-lyso-sn-glycerol-3-phosphocholine + acetate
-
-
-
?
1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O
1-O-alkyl-2-lyso-sn-glycerol-3-phosphocholine + acetate
-
hydrolysis of human platelet-activating factor PAF to lyso-PAF
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
1-palmitoyl-sn-glycero-3-phosphocholine + cholesteryl oleate
-
in presence of apoA-I lipoprotein
-
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
1-palmitoyl-sn-glycero-3-phosphocholine + cholesteryl oleate
-
in presence of apoA-I lipoprotein
-
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
?
-
cholesterol in HDL of 80 and 93 A, the latter gives a 12fold higher activity with recombinant His-tagged enzyme
-
-
?
cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine
cholesteryl oleate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine
cholesteryl oleate + 1-palmitoylglycerophosphocholine
-
-
-
?
cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine
cholesteryl oleate + 1-palmitoylglycerophosphocholine
-
-
-
?
L-alpha-phosphatidylcholine type XVI-E + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
?
L-alpha-phosphatidylcholine type XVI-E + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
enzyme activities with apolipoprotein A-I and Apo AI-derived peptides DRV, KLL, and VLES, overview
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
?
phosphatidylcholine + a sterol
1-acylglycerophosphocholine + a sterol ester
-
-
-
?
phosphatidylcholine + cerebrosterol
1-acylglycerophosphocholine + cerebrosteryl ester
-
-
-
?
phosphatidylcholine + cerebrosterol
1-acylglycerophosphocholine + cerebrosteryl ester
i.e. (24S)-hydroxycholesterol
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesterol ester
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesterol ester
-
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
proteoliposome substrates
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
the enzyme is responsible for esterification of cholesterol in high density lipoprotein HDL to prevent diffusion of cholesterol back to the cell
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
purified recombinant enzyme
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
proteoliposome substrates
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
proteoliposome substrates
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
LCAT contributes significantly to the apoB lipoprotein cholesteryl ester pool, enzyme role in lipid and lipoprotein metabolism in plasma, detailed overview
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
activity with apoB lipoprotein, overview
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
proteoliposome substrates
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
LCAT contributes significantly to the apoB lipoprotein cholesteryl ester pool, enzyme role in lipid and lipoprotein metabolism in plasma, detailed overview
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
activity with apoB lipoprotein, overview
-
-
?
phosphatidylcholine + cholesterol
1-acylglycerophosphocholine + cholesteryl ester
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT is a serum enzyme that catalyses esterification of free cholesterol to produce cholesteryl ester. Cholesterol for this reaction comes from peripheral tissues and the donor of the acyl group is lecithin from the high density lipoprotein, HDL, particles
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and subsequently transfers and esterifies the fatty acid to free cholesterol with apolipoprotein A-I as cofactor
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT is a plasma enzyme produced by the liver, that catalyzes the conversion of cholesterol to cholesteryl esters on lipoproteins by the transacylation of fatty acid from the sn-2 position of phosphatidylcholine to the 3-hydroxyl group on the A-ring of cholesterol
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT is critical in the metabolism of HDL
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
enzyme with bound activator apolipoprotein A-I, structure-function relationship and analysis, molecular dynamics and simulations, overview
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and subsequently transfers and esterifies the fatty acid to the 3beta-hydroxy group of free cholesterol
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and subsequently transfers and esterifies the fatty acid to the beta3-hydroxyl group of free cholesterol using apolipoprotein A-I as cofactor
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and subsequently transfers and esterifies the fatty acid to the 3beta-hydroxy group of free cholesterol
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
phosphatidylcholine + cholesterol
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
-
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
wide specificity for acyl acceptor: sterols with a beta-configuration at carbon-3 and trans fused A /B rings
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
wide specificity for acyl acceptor: sterols with a beta-configuration at carbon-3 and trans fused A /B rings
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
wide specificity for acyl acceptor: sterols with a beta-configuration at carbon-3 and trans fused A /B rings
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
cholesterol as substrate
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
cholesterol as substrate
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
cholesterol as substrate
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
cholesterol as substrate
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
cholesterol as substrate
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
water as acyl acceptor: phospholipase activity
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
water as acyl acceptor: phospholipase activity
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
phosphatidylethanolamine, dimethylphosphatidylethanolamine, lecithins containing different acyl chain lengths and degrees of saturation
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
phosphatidylethanolamine, dimethylphosphatidylethanolamine, lecithins containing different acyl chain lengths and degrees of saturation
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
acyl donor: high degree of specificity for phospholipids containing a basic nitrogen atom
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
predominant but incomplete specificity for reaction at position 2 of mixed lecithins
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
predominant but incomplete specificity for reaction at position 2 of mixed lecithins
-
r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure
-
?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
-
important in metabolism and structure of plasma lipoproteins
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phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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?
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylcholine + sterol
1-acylglycerophosphocholine + sterol ester
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r
phosphatidylethanolamine + cholesterol
cholesteryl ester + lysophosphatidylethanolamine
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?
phosphatidylethanolamine + cholesterol
cholesteryl ester + lysophosphatidylethanolamine
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?
phosphatidylethanolamine + cholesterol
cholesteryl ester + lysophosphatidylethanolamine
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?
additional information
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bacterial enzyme is not specific for phosphatidylcholine, it can use human erythrocyte membrane substrates, 2-positional specificity as acyltransferase
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?
additional information
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symmetrical diacyl phosphatidylcholines are not substrates for human or rat enzymes
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?
additional information
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long-chain primary alcohols can act as acyl acceptors
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?
additional information
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long-chain primary alcohols can act as acyl acceptors
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?
additional information
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no significant activity with 1-sn-phosphorylcholinediacylglycerol
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?
additional information
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mechanism of LAT reaction is similar to that of LCAT, probably involving an acyl-enzyme intermediate, 2-acyl isomers of lysolecithin and lysophosphatidylethanolamine can act as substrates in the LAT reaction
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?
additional information
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enzyme hydrolyses ester linkage at carbon-2 position of phosphatidylcholine
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?
additional information
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lecithin-cholesterol single bilayer vesicles as substrate
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?
additional information
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lecithin-cholesterol single bilayer vesicles as substrate
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?
additional information
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lecithin-cholesterol single bilayer vesicles as substrate
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?
additional information
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enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure
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additional information
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enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure
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?
additional information
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enzyme forms higher aggregates with HDL and apolipoprotein A-I in plasma, esterification of cholesterol and binding to cholesteryl ester transfer protein are required for reverse cholesterol transport from peripheral cells to plasma
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?
additional information
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substrate binding study of wild-type and mutant enzymes
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?
additional information
?
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the enzyme accepts a variety of acceptor substrates including sterols and several other alcohols, at high lysophosphatidylcholine concentrations the enzyme catalyzes the reverse reaction producing lecithin on LDL, i.e. exhibiting lysolecithin acyltransferase activity, in absence of an appropriate sterol acceptor, the enzyme exhibits phospholipase A2 or esterase activity liberating free fatty acids
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?
additional information
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the enzyme activity is largely influenced by HDL particle size and negative net charge of the cofactor apolipoprotein A-I, overview
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?
additional information
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the fatty acid substrate specificity is determined by residues E149, Y292, and W294, enzyme also shows phospholipase A2 activity with the substrates
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?
additional information
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the substrates are present in a ratio of 100:10:1 for phosphatidylcholine, cholesterol, and apolipoprotein A-I in the assay mixture
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additional information
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a frameshift mutation of the LCAT gene is associated with renal failure with proteinuria, corneal opacity, and anemia in familial LCAT deficiency
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additional information
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the activity and fatty acid specificity of LCAT may be altered during the inflammatory response
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?
additional information
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the enzyme is involved in development of atherosclerosis
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?
additional information
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the enzyme is responsible for the cholesterol transport, controlling the flow of cholesterol from peripheral tissues to the liver
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additional information
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the rare enzyme genetic disorder, familial LCAT deficiency, leads to altered plasma lipid and lipoprotein levels, corneal opacities and proteinuria with renal failure, phenotype analysis, overview
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?
additional information
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comparison of conformational changes upon substrate binding of wild-type enzyme and mutant T123I, a naturally occuring mutation involved in the fish eye disease, overview
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?
additional information
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about 75% of plasma LCAT is associated with HDL
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?
additional information
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HDL2 and HDL3-phospholipids, HDL2-cholesterol concentrations and LCAT activity are reduced in preganancy-induced hypertension and in chronic hypertensive mothers, as well as in their small for gestational age, SGA, newborns, overview
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?
additional information
?
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plasma high-sensitivity C-reactive protein, CRP, is correlated inversely with HDL-C and apo A-I, and positively with LCAT activity, interaction between apo A-I and LCAT activity on CRP, interaction analysis, overview
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?
additional information
?
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cholesteryl ester-rich HDL particles bind LCAT effectively and contain apolipoprotein A-I and ample phosphatidylcholine and unesterified cholesterol substrates but exhibit relatively low reactivity with LCAT since the cholesteryl ester products cannot be removed from the active site
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?
additional information
?
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study of enzyme interactions with macromolecules and conformational changes upon binding by labeling of tryptophan with 8-anilino-1-naphthalenesulfonate, ANS, steady-state and time-resolved fluorescence emission and anisotropies, Foerster resonance energy transfer, FRET, mechanism, method development and evaluation, overview
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?
additional information
?
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the human enzyme prefers phospholipids with 18:1 or 18:2 fatty acids
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?
additional information
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cholesterol and (24S)-hydroxycholesterol compete for enzyme activity
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?
additional information
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structure/function requirements for the apolipoprotein A-I and lecithin cholesterol acyltransferase interaction loop of HDL, binding studies with recombinant wild/type apoA-I and apoA-I mutants P165A, Y166A, Y166E, Y166F, Y166N, S167A, D168A, Y192F, and Y192L. Loss of LCAT activation caused by apoA-I Y166A or Y166F mutations is at least partially due to the weaker binding between LCAT and apoA-I within these rHDL. Both Vmax of Y166F and Y166A are 20% lower than that of wild-type apoA-I, and the apparent Km are about 2-6fold higher than wild-type. rHDL formed with either apoA-I Y192F or Y192L mutants show normal LCAT activity. Incubation of hLCAT with rHDL formed using the apoA-I mutants P165A, Y166A, Y166F, S167A and D168A all show significant decreases in LCAT activity of 40%-60% compared with the wild-type
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?
additional information
?
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structure/function requirements for the apolipoprotein A-I and lecithin cholesterol acyltransferase interaction loop of HDL, binding studies with recombinant wild/type apoA-I and apoA-I mutants P165A, Y166A, Y166E, Y166F, Y166N, S167A, D168A, Y192F, and Y192L. Loss of LCAT activation caused by apoA-I Y166A or Y166F mutations is at least partially due to the weaker binding between LCAT and apoA-I within these rHDL. Both Vmax of Y166F and Y166A are 20% lower than that of wild-type apoA-I, and the apparent Km are about 2-6fold higher than wild-type. rHDL formed with either apoA-I Y192F or Y192L mutants show normal LCAT activity. Incubation of hLCAT with rHDL formed using the apoA-I mutants P165A, Y166A, Y166F, S167A and D168A all show significant decreases in LCAT activity of 40%-60% compared with the wild-type
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?
additional information
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development and evaluation of a simple but sensitive fluorescence assay method to accurately detect the esterification activity of lecithin:cholesterol acyltransferase, with the potential of the activity assay to be used as a screening test and clinical study tool. An amphiphilic peptide in place of apolipoprotein A-I is used as the lipid emulsifier and enzyme LCAT activator
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?
additional information
?
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development and evaluation of a simple but sensitive fluorescence assay method to accurately detect the esterification activity of lecithin:cholesterol acyltransferase, with the potential of the activity assay to be used as a screening test and clinical study tool. An amphiphilic peptide in place of apolipoprotein A-I is used as the lipid emulsifier and enzyme LCAT activator
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?
additional information
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high-density lipoprotein subfraction 3 functions as highly effective substrate
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additional information
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LCAT adds cholesteryl ester to LDL in mice and is important in remodeling VLDL to LDL, LCAT deficiency significantly decreases the cholesteryl ester percentage and significantly increases the phospholipid percentage of LDL, overview
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?
additional information
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the mouse enzyme prefers phospholipids with 20:4 fatty acids
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?
additional information
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for the endogenous assay, the plasma sample is equilibrated with human serum albumin-3H cholesterol emulsion
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?
additional information
?
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for the exogenous substrate assay, proteoliposomes containing 14C-labeled cholesterol, egg PC, and apo AI at molar ratios of 15:300:1 are prepared. Plasma sample is incubated with proteoliposome substrate
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?
additional information
?
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increased HDL cholesterol after LCAT lecithin-cholesterol acyltransfer activity inhibits progression of atherosclerosis and induces cholesterol unloading in complex lesions in rabbits, overview
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?
additional information
?
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LCAT catalyzes the transacylation of a fatty acid at the sn-2 position of phosphatidylcholine preferentially to the free 3beta-hydroxyl group of cholesterol in HDL and to a lesser extent in LDL particles
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?
additional information
?
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symmetrical diacyl phosphatidylcholines are not substrates for human or rat enzymes
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?
additional information
?
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rat enzyme has similar substrate specificity for phosphatidylcholines than human enzyme
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?
additional information
?
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lecithin-cholesterol single bilayer vesicles as substrate
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?
additional information
?
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the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase
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?
additional information
?
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the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase
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?
additional information
?
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the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase
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?
