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2.3.1.47: 8-amino-7-oxononanoate synthase

This is an abbreviated version!
For detailed information about 8-amino-7-oxononanoate synthase, go to the full flat file.

Word Map on EC 2.3.1.47

Reaction

pimeloyl-[acyl-carrier protein]
+
L-alanine
=
8-Amino-7-oxononanoate
 +
CO2
 +
holo-[acyl-carrier protein]

Synonyms

7,8-diaminopelargonic acid aminotransferase, 7-KAP synthetase, 7-keto-8-aminopelargonate synthase, 7-keto-8-aminopelargonate synthetase, 7-keto-8-aminopelargonic acid synthase, 7-keto-8-aminopelargonic acid synthetase, 7-keto-8-aminopelargonic synthetase, 7-oxo-8-aminononanoate synthase, 8-amino-7-oxononanoate synthase, 8-amino-7-oxopelargonate synthase, AON synthase, AONS, AOP synthase, AtbioF, BioF, bioF protein, DAPA AT, KAPA synthase, KAPA synthetase, KAPAS, More, SxtA, synthase, 7-oxo-8-aminononanoate, synthetase, 7-keto-8-aminopelargonate

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.47 8-amino-7-oxononanoate synthase

Purification

Purification on EC 2.3.1.47 - 8-amino-7-oxononanoate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography
-
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration
-
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation
-
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography
immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration
Thermosynechococcus vestitus
-
maltose binding protein affinity chromatography
-
native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Ni-NTA column chromatography
partial
recombinant enzyme from Escherichia coli strain BL21-Gold (DE)
recombinant from E. coli
recombinant from E. coli overproducing strain
-
recombinant from overexpressing E. coli strain HMS174 (DE3)
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiinity chromatography and gel filtration to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography, desalting gel filtration, and anion exchange chromatography followed by gel filtration, to over 95% purity