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2.3.1.48: histone acetyltransferase

This is an abbreviated version!
For detailed information about histone acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.48

Reaction

acetyl-CoA
+
[protein]-L-lysine
=
CoA
 +
[protein]-N6-acetyl-L-lysine

Synonyms

AAPatA, acetyltransferase, histone, Ada2/Ada3/Gcn5 complex, AIB1, amino acid sensing acetyltransferase, AmiPatA, Amir 5672, ARD1, ARD1 acetyltransferase, arginine methyltransferase A, arginine methyltransferase B, arrest defective 1, arrest defective protein 1 acetyltransferase, At3g14980, ATAC2, ATase1, ATase2, cAMP-regulated protein lysine acetyltransferase, Cbp, CBP histone acetyltransferase, CBP/p300, chameau, circadian locomoter output cycles protein kaput, CLOCK, CREB binding protein, CREB-binding protein, CREBBP, east1, ELO3, ELONGATA3, elongator protein 3, ELP3, enhancer-of-asymmetric leaves-two1, Enok, EP300, Esa1, factor acetyltransferase, FAT, Gcn5, GCN5 family KAT, Gcn5 HAT, GCN5 lysine acetyltransferase, GCN5 N acetyltransferase 5, Gcn5 related N-acetyltransferase, GCN5-like acetyltransferase, GCN5-like enzyme, GCN5-related N-acetyltransferase, Gcn5/PCAF histone acetyltransferase, GCN5b, GCN5L2, general control non-derepressible 5, general control non-repressed protein5, general control nonderepressible 5, general control nonrepressed-protein 5, general control of amino acid synthesis 5, GNAT, GNAT-related histone acetyltransferase complex, GTF3C4, H4 lysine acetyltransferase, HAC1, HAG1, HAG3, HAG4, HAG5, HAM1, HAM2, hARD1, HAT, HAT-B, HAT-B complex, Hat1, Hat1p, Hat2, HatB3.I, HBO1, histone (H4 K16) acetyltransferase, histone acetokinase, histone acetyl transferase, histone acetylase, histone acetyltransferase, histone acetyltransferase 1, histone acetyltransferase AtGCN5, histone acetyltransferase B, histone acetyltransferase Tip60, histone acetyltransferase-1, histone acetyltransferases, histone H3 acetyltransferase, histone H4 acetyltransferase, histone H4 lysine 16 acetyltransferase, histone transacetylase, histone/protein lysine acetyltransferase, HIV-1 Tat-interactive protein, hMOF, Hpa2, Hpa3, human acetylase binding to ORC1, Idm1, K (lysine) acetyltransferase 8, K(lysine) acetyltransferase 8, KAT, KAT10, KAT11, KAT12, KAT13A, KAT13B, KAT13C, KAT13D, KAT2, Kat2A, KAT2A/GCN5, Kat2b, KAT3A, KAT3B, KAT4, KAT5, KAT6, KAT6A, KAT6B, KAT7, KAT8, KAT9, Lsy-12, lysine acetyltransferase, lysine acetyltransferase 2, lysine acetyltransferase 2A, lysine acetyltransferase 2B, lysine acetyltransferase 5, lysine acetyltransferase 8, lysine acetyltransferase complex, lysine acetyltransferase p300, lysine acetyltransferases, lysine-acetyltransferase, Mof, monocytic leukemia zinc finger, monocytic leukemia zinc finger protein, More, Morf, MORF histone acetyltransferase, MOZ, MOZ histone acetyltransferase, Moz related factor, Mst1, MtPat, Myb-binding protein 1A, MYBBP1A, MYST, MYST protein lysine acetyltransferase, MYST-related histone acetyltransferase complex, MYST1, MYST2, MYST3, MYST4, N(alpha)-acetyltransferase 10, N-acetyltransferase, N-terminal acetyltransferase, NAA10, NAT, NAT4, NCoA-1, NCoA-3, NCOAT, Nepsilon-lysine acetyltransferase, NuA4, NuA4 histone acetyltransferase, nuclear cytoplasmicO-GlcNAcase and acetyltransferase, nuclear receptor coactivator 1, nuclear receptor coactivator 3, nucleosome-histone acetyltransferase, Nut1, p/CAF, P/CAF histone acetyltransferase, p160, p300, p300 HAT, p300 histone acetyltransferase, p300/CBP, p300/CBP associated factor, p300/CBP histone acetyltransferase, P300/CBP-associated factor, p300/CBP-associated factor (pCAF), p300HAT, PA4534, Pat, PCAF, PCAF histone acetyltransferase, PF11_0192, PfGCN5, PfGCN5 HAT, Piccolo NuA4 complex, protein acetyl-transferase, protein acetyltransferase, Qkf, regulator of Ty1 transposition protein 109, RmtA, RmtB, RPD3, Rtt109, Rv0998, SAS complex, Sas2, Sas3, SRC1, SRC3, Sven 0867, SvePatA, TAF, TAF1, Tat interacting protein of 60 kDa, Tat-interactive protein, 60 kDa, TBP1, TFIIIC90, tGCN5, TgGCN5b, TgMYST-B, Tip60, tumor suppressor histone H3 lysine 23 acetyltransferase, type B histone acetyltransferase, YfmK, zMoz

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.48 histone acetyltransferase

Purification

Purification on EC 2.3.1.48 - histone acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity column chromatography and Superdex 75 gel filtration
-
cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation
-
enzyme form A
-
enzyme form B
enzyme forms I and II
-
HisTrap column chromatography and Superdex 75 gel filtration
native Rtt109, in complex with the histone chaperone Vps75
-
native SAS complex and recombinant SAS complex, the latter with Sas2 protein being His-tagged
-
Ni-NTA column chromatography and Superdex S200 gel filtration
Ni-NTA resin column chromatography
partial
partial enzyme forms A and B
-
partial from viral nucleoprotein from infected african green monkey cell line CV-1
Betapolyomavirus macacae
-
partial, acyltransferase B
-
partial, enzyme form A and B
-
partial, enzyme form B
-
partial, enzyme forms A and B
-
partial, enzyme forms A1, A2, and B
-
partial, enzyme forms DB and B
-
recombinant Atac2 fused to two HA and two FLAG tags from nuclear extracts of HEK-293 cells and murine C2C12 cells by M2-agarose affinity chromatography and gel filtration
recombinant catalytic domain of Gcn5 protein
-
recombinant catalytic domain of PCAF protein from E. coli
-
recombinant enzyme domains of PCAF and Gcn5
-
recombinant enzyme form B 55000 kDa subunit as His-tagged protein from E. coli
recombinant from Sf9 insect cells via baculovirus infection, FLAG-tagged MORF protein
recombinant GST- and FLAG-tagged KAT6B histone acetyltransferase domain from Escherichia coli by affinity chromatography
recombinant GST-tagged enzyme from BSC40 cells by glutathione affinity chromatography
-
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography
-
recombinant His-tagged ARD1/Naa10 from Escherichia coli by nickel affinity chromatography, anion exchange chromatography, and gel filtration. After purification, rhARD1/NAA10 mainly exists in a high oligomeric state and has only a few monomers. Recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged catalytic domain of PCAF protein from E. coli
-
recombinant His-tagged enzyme and GST-tagged enzyme from Escherichia coli strain BL21 by nickel and glutathione affinity chromatography, respectively
recombinant His-tagged enzyme from E. coli
-
recombinant His-tagged enzyme in Ada2/Ada3/Gcn5 subcomplex of SAGA or in the Piccolo NuA4 complex from Escherichia coli strain BL21(DE3) by metal affinity chromatography followed by anion or cation exchange chromatography, and hydrophobicinteraction chromatography, method development, overview
-
recombinant His-tagged full-length enzyme and catalytic domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged hARD1/NAA10 enzyme by nickel affinity chromatography, with or without anion exchange chromatography, followed by gel filtration, and dialysis, the lysine acetylation activity of hARD1/NAA10 is well maintained until the elution step, but dramatically diminished after overnight dialysis. rhARD1/NAA10 aggregates during purification
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography
-
recombinant N-terminally HA-tagged enzyme TgGCN5b from parasites overexpressing an ectopic enzyme copy by immunoaffinity chromatography
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through TEV protease, gel filtration, and ultrafiltration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography
recombinant wild-type Sas3 protein and mutants as GST-fusion proteins from E. coli
-
tGcn5 domain, recombinant from E. coli
-
using talon metal affinity chromatography, anion-exchange chromatography and cation-exchange chromatography. Typical yields of the yeast Ada2/Ada3/Gcn5 complex are approximately 0.5-0.8 mg/L of culture
-
using talon metal affinity chromatography, anion-exchange chromatography, cation-exchange chromatography and hydrophobic interaction chromatography. Typical yields of the Piccolo NuA4 complex are of the 1-3 mg per liter of culture
-
wild-type and recombinant HA-tagged enzyme