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2.3.1.5: arylamine N-acetyltransferase

This is an abbreviated version!
For detailed information about arylamine N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.5

Reaction

acetyl-CoA
+
an arylamine
=
CoA
 +
an N-acetylarylamine

Synonyms

(BACAN)NAT3, (MYCAB)NAT1, 2-naphthylamine N-acetyltransferase, 4-aminobiphenyl N-acetyltransferase, ABW01_24350, acetyl CoA-arylamine N-acetyltransferase, acetyltransferase, 2-naphthylamine N-, acetyltransferase, 4-aminobiphenyl, acetyltransferase, arylamine, acetyltransferase, p-aminosalicylate N-, acetyltransferase, procainamide N-, acetyltransferase, serotonin N-, arylamine acetylase, arylamine acetyltransferase, arylamine N-acetyl transferase, arylamine N-acetyltransferase, arylamine N-acetyltransferase 1, arylamine N-acetyltransferase 2, arylamine N-acetyltransferase C, arylamine N-acetyltransferase I, arylamine N-acetyltransferase type 1, arylamine N-acetyltransferase type 2, arylamine N-acetyltransferase type I, arylamine-N-acetyltransferase 1, BanatA, BanatB, BanatC, beta-naphthylamine N-acetyltransferase, indoleamine N-acetyltransferase, MlNAT1, MMNAT, More, MSNAT, N-acetyltransferase, N-acetyltransferase a, N-acetyltransferase b, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, NAT, NAT 1, NAT-a, NAT-b, NAT1, NAT2, NAT2*1, NAT2*2, NAT3, NAT31, NfNAT, p-aminosalicylate N-acetyltransferase, PANAT, rhesus NAT2, serotonin acetyltransferase, serotonin N-acetyltransferase, STNAT, TBNAT, Tpau_4046, UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase, UDP-D-Glc(2NAc3N)A 3-N-acetyltransferase, WbpD

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.5 arylamine N-acetyltransferase

Specific Activity

Specific Activity on EC 2.3.1.5 - arylamine N-acetyltransferase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000000001
0.000000002
0.000000003
0.000000004
0.000000005
0.000000006
0.000000007
-
2-aminofluorene, pH 7.4, 37°C
0.000000008
-
4-aminosalicylic acid, pH 7.4, 37°C
0.00000001
0.000000014
-
strain YG1024, p-aminobenzoic acid, pH 7.4, 37°C
0.00000002
0.00000004
-
strain TA1538, 2-aminofluorene, pH 7.4, 37°C
0.00000005
0.00000006
0.000000075
isoform NAT 2, 4-aminosalicylic acid, pH 7.4, 37°C
0.00000009
0.0000001
0.00000014
0.00000015
-
2-aminofluorene, pH 7.4, 37°C
0.00000016
0.00000027
0.00000032
-
2-aminofluorene, pH 7.4, 37°C
0.00000033
-
5-aminosalicylic acid, pH 7.4, 37°C
0.00000059
-
5-aminosalicylic acid, pH 7.4, 37°C
0.00000061
-
5-aminosalicylic acid, pH 7.4, 37°C
0.00000099
isoform NAT 2, 2-aminofluorene, pH 7.4, 37°C
0.00000106
-
2-aminofluorene, pH 7.4, 37°C
0.00000189
isoform NAT 2, 5-aminosalicylic acid, pH 7.4, 37°C
0.0000022
-
5-aminosalicylic acid, pH 7.4, 37°C
0.00000241
isoform NAT 2, sulfamethazine, pH 7.4, 37°C
0.00000568
isoform NAT 1, procainamide, pH 7.4, 37°C
0.0000083
isoform NAT 1, sulfamethazine, pH 7.4, 37°C
0.0000208
-
strain YG1024, 5-aminosalicylic acid, pH 7.4, 37°C
0.0000275
-
strain YG1024, 2-aminofluorene, pH 7.4, 37°C
0.0001
0.000162
-
cytosol
0.0002
0.0005
0.00052
isoform NAT 1, 2-aminofluorene, pH 7.4, 37°C
0.00058
isoform NAT 1, p-aminobenzoic acid, pH 7.4, 37°C
0.000592
-
cytosol, 37°C
0.0006
0.00068
-
cytosol
0.0007
0.001
-
breast cancer cell line MCF-7, estrogen receptor (ER)-positive
0.00109
isoform NAT 1, 4-aminosalicylic acid, pH 7.4, 37°C
0.00117
isoform NAT 1, 5-aminosalicylic acid, pH 7.4, 37°C
0.0013
mutant GRSG
0.0014
mutant GRSG
0.00161
-
HeLa cell + N-acetyl-L-cysteine + 4-nitrosobiphenyl
0.00172
-
HeLa cell + 4-nitrosobiphenyl
0.0018
-
MCF-7 cell
0.0021
0.00408
-
in HeLa cells, substrate: p-aminosalicylic acid
0.0044
-
in HeLa cells, substrate: p-aminobenzoic acid
0.00454
-
HeLa cell + N-acetyl-L-cysteine
0.00466
-
HeLa cell
0.00478
-
cytosol
0.005
-
breast cancer cell line T47D, estrogen receptor (ER)-positive
0.0054
-
T-47D cell
0.0055
mutant GG
0.006
mutant GG
0.008
-
enzymatic activity found in peripheral blood mononuclear cell of investigated donors: 8-23.5 nmol/mg/min
0.0104
effect of androgen receptor expression in PC-3 cells, control, plus R1881
0.0105
-
enzymatic activity found in HepG2 cells
0.0109
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1, plus R1881
0.011
0.0113
effect of androgen receptor expression in PC-3 cells, control
0.0115
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1
0.013
substrate: 2-aminofluorene
0.014
HeLa cell
0.015
0.022
-
mutant G129V
0.023
0.0234
-
enzymatic activity found in monocyte-derived dendritic cells of investigated donors: 23.4-26.6 nmol/mg/min
0.024
substrate: 4-aminobiphenyl
0.02463
substrate: 5-aminosalicylate
0.029
0.034
-
mutant G129T
0.038
22Rv1 cell, plus R1881, plus flutamide
0.042
22Rv1 cell, plus DMSO
0.045
LNCaP cell
0.046
22Rv1 cells and LNCAP cells (both androgene-positive lines) show a high basal level of NAT1 activity between 46-51 nmol/min/mg protein
0.05
22Rv1 cell
0.052
substrate: benzidine
0.054
-
cytokine mixture, 2.5 microM p-aminobenzoic acid
0.079
substrate: 4-aminoveratrole
0.08
22Rv1 cell, plus R1881, 24h
0.087
substrate: hydralazine
0.097
-
cytokine mixture, 7.5 microM p-aminobenzoic acid
0.108
-
control, 2.5 microM p-aminobenzoic acid
0.111
0.124
-
cytokine mixture, 40 microM p-aminobenzoic acid
0.13
22Rv1 cell, treated with R1881
0.14
22Rv1 prostate cells upon treatment with 100 nmol of synthetic androgen R1881 for 24h
0.145
-
2-aminofluorene
0.146
-
control, 7.5 microM p-aminobenzoic acid
0.168
0.177
-
sulfamethazine
0.19
-
after streptomycin sulfate treatment
0.2
-
control, 40 microM p-aminobenzoic acid
0.202
-
breast cancer cell line ZR-75-1, estrogen receptor (ER)-positive
0.2022
-
ZR-75-1 cell
0.237
-
recombinant NAT1, substrate: p-aminobenzoic acid
0.25
-
cell extract
0.29
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzenesulfonic acid
0.33
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzaldehyde
0.337
-
recombinant NAT1, substrate: p-aminosalicylic acid
0.372
0.38
-
purification step ammonium sulfate precipitation
0.41
-
2-aminofluorene
0.416
0.47
purified recombinant enzyme, with substrate 2-aminophenol
0.532
-
4-aminobenzoic acid L-glutamate, pH 8, 37°C
0.55
-
-
0.567
0.602
0.603
0.629
0.641
-
4-methoxyphenylhydrazine, pH 8, 37°C
0.67
-
2-aminofluorene
0.75
-
4-aminobenzoic acid
0.841
0.91
-
2-aminofluorene
0.969
1
-
sulphisoxazole, pH 8, 37°C
1.1
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzoic acid or 2-amino-4-methylphenol
1.31
-
sulphametoxazole, pH 8, 37°C
1.744
1.908
donor acetyl-CoA, pH 7.5, temperature not specified in the publication
12.05
-
4-hexyloxyaniline, pH 8, 37°C
13.03
4-aminofluorene
13.47
-
4-anisidine, pH 8, 37°C
13.8
-
4-iodoaniline, pH 8, 37°C
14
-
4-chorobenzoic hydrazide, pH 8, 37°C
14.02
-
-
18.66
-
4-aminosalicylate, pH 8, 37°C
19.24
-
4-ethoxyaniline, pH 8, 37°C
2.012
donor propionyl-CoA, pH 7.5, temperature not specified in the publication
2.324
2.54
-
4-aminobenzoic acid
2.76
-
4-trifluoromethylaniline, pH 8, 37°C
2.878
2.89
-
2-aminofluorene
21.97
-
4-phenoxyaniline, pH 8, 37°C
23.73
beta-naphthylamine
24.63
5-aminosalicylate
24.91
2-aminobiphenyl
25.66
-
4-butoxyaniline, pH 8, 37°C
26
-
purification step DEAE Toyopearl column
27
-
purification step DEAE Toyopearl column
28
-
thrombin cleaved fusion protein
29.55
3.109
3.53
donor malonyl-CoA, pH 7.5, temperature not specified in the publication
3.679
4.14
-
4-chloroaniline, pH 8, 37°C
4.152
-
mutant G129S
4.758
-
mutant G129A
44.51
-
4-aminoveratrole, pH 8, 37°C
44.71
5.078
5.71
-
4-bromoaniline, pH 8, 37°C
5.852
52.33
benzidine
6.76
-
3,4-dichloroaniline, pH 8, 37°C
7
-
purification step POROS 20PE column
7.3
-
purification step DE52-cellulose column
79.39
4-aminoveratrole
87.76
hydralazine
additional information