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2.3.1.50: serine C-palmitoyltransferase

This is an abbreviated version!
For detailed information about serine C-palmitoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.50

Reaction

palmitoyl-CoA
+
L-serine
=
CoA
 +
3-dehydro-D-sphinganine
 +
CO2

Synonyms

3-oxosphinganine synthetase, acyl-CoA:serine C-2 acyltransferase decarboxylating, LCB1, LCB2, LCB2a, LCB2b, More, palmitoyltransferase, serine, serine palmitoyl transferase, serine palmitoyltransferase, serine palmitoyltransferase 1, serine palmitoyltransferase a, serine-palmitoyl transferase, serine-palmitoyltransferase, SPT, SPT1, SPT2, SPT3, SPTase, SPTLC1, SPTLC2, ssSPT, ssSPTa, Tsc3

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.50 serine C-palmitoyltransferase

Purification

Purification on EC 2.3.1.50 - serine C-palmitoyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography
Rhizorhabdus wittichii
-
immobilized metal ion affinity chromatography (Ni2+), gel filtration
native and recombinant from E. coli, LCB1 and LCB2 co-immunoprecipitate
Ni-NTA column chromatography
nickel resin column chromatography
of affinity-peptide tagged SPT complex
-
purified as a water-soluble active homodimer
purified as water-soluble active homodimers
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and enzyme and mutants V246M and G385F from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant SPT from Escherichia coli strain BL21(DE3)
Superdex S200 gel filtration
-