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2.3.1.51: 1-acylglycerol-3-phosphate O-acyltransferase

This is an abbreviated version!
For detailed information about 1-acylglycerol-3-phosphate O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.51

Reaction

acyl-CoA
+
1-acyl-sn-glycerol 3-phosphate
=
CoA
 +
1,2-diacyl-sn-glycerol 3-phosphate

Synonyms

1-acyl-sn-glycero-3-phosphate acyltransferase, 1-acyl-sn-glycero-3-phosphate acyltransferase 1, 1-acyl-sn-glycero-3-phosphate acyltransferase 9, 1-acyl-sn-glycerol 3-phosphate acyltransferase, 1-acyl-sn-glycerol-3-phosphate acyltransferas, 1-acyl-sn-glycerol-3-phosphate acyltransferase, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase, 1-acylglycero-3-phosphate acyltransferase, 1-acylglycerol 3-phosphate O-acyltransferase, 1-acylglycerol-3-phosphate acyltransferase, 1-acylglycerol-3-phosphate-O-acyltransferase, 1-acylglycerol-3-phosphate-O-acyltransferase 2, 1-acylglycerol-3-phosphate-O-acyltransferase isoform10/glycerol-3-phosphate acyltransferase isoform 3, 1-acylglycerol-3-phosphate-O-acyltransferase-1, 1-acylglycerolphosphate acyltransferase, 1-acylglycerophosphate acyltransferase, 1-Agpat2, ABHD5, acyl-CoA: sn-1-acyl-glycerol-3-phosphate acyltransferase, acyl-CoA:1-acylglycerol-sn-3-phosphate acyltransferase, acyl-CoA:lysophosphatidic acid acyltransferase, acyl-CoA:lysophospholipid acyltransferase, acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase, acylglycerophosphate acyltransferase 4, acyltransferase, 1-acylglycerol phosphate, AGPAT, AGPAT/PlsC, AGPAT1, AGPAT10/GPAT3, AGPAT11, AGPAT2, AGPAT3, AGPAT4, AGPAT5, AGPAT6/GPAT4, AGPAT9, ALE1, At4g24160, AtLPAAt2, CGFI-58, cgi-58 protein, CGI-58/ABHD5, CHLREDRAFT_174358, coenzyme A-dependent lysophosphatidic acid, CrLPAAT1, CT775, CT_775, endoplasmic reticulum-located LPAT, ER-located LPAT, glycerol-3-phosphate acyltransferase-1, GPAT-1, ICT1 protein, LOA1, LPA acyltransferase, LPAAT, LPAAT-beta, LPAAT1, LPAAT2, LPAAT3, LPAAT4, LPAATbeta, LPAATdelta, LPAT, LPCAT1, LPCAT2, lysophosphatidic acid acyl transferase, lysophosphatidic acid acyltansferase, lysophosphatidic acid acyltransferase, lysophosphatidic acid acyltransferase 3, lysophosphatidic acid acyltransferase beta, lysophosphatidic acid ayltransferase, lysophosphatidic acid-acyltransferase, lysophosphatidic acid–specific acyltransferase, lysophosphatidyl acyltransferase, lysophosphatidyl acyltransferase 4, lysophosphatidylcholine acyltransferase 1, lysophospholipid acyltransferase, lysophospholipid:acyl-CoA acyltransferase, mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4, mAGPAT5, More, oleoyl-CoA lysophosphatidic acid acyltransferase, PCC8801_1274, plastidial LPAAT, plastidial lysophosphatidic acid acyltransferase, PlsC, PlsC4, PrLPAAT1, Scl1, ScLPAAT, SLC1, Slc1p, Slc4p, SlPlsC1, SMc00714 protein, SnGlycerol 3-P acyltransferase, TmPlsC, TM_1693, YPR139c

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.51 1-acylglycerol-3-phosphate O-acyltransferase

Cloned

Cloned on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AGPAT10/GPAT3, expression in CHO cells endoplasmic reticulum, and as AGPAT-active enzyme in HEK-293 cells via transfection with an adenoviral vector, overexpression of the AGPAT-active enzyme in Huh-7 cells depleted of endogenous AGPAT activity by shRNA-AGPAT2-lentivirus
-
amino acid sequence data used to isolate a full-length clone from a coconut endosperm cDNA library, expression of the cDNA in Escherichia coli
-
Escherichia coli C43(DE3) is transformed with a pET21a-derived expression vector (pET21a-SlPlsC1) that encoded SlPlsC1 fused with a C-terminal hexahistidine-tag
expressed in Arabidopsis thaliana
expressed in Baculovirus-Sf9 insect cells, pESC-URA yeast expression vector with a c-myc-epitope, transformation into Escherichia coli after site-directed mutagenesis
expressed in CHO-K1 cells
expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed
expressed in Escherichia coli, overexpression in Saccharomyces cerevisiae, expression plasmid pPS189
-
expressed in Escherichia coli, plasmids pCR2.1, pDrive, pcDNA 3.1(C)neo, construction of the wild-type fusion protein with GFP, overexpression in CHO-cells, coexpression in CHO-cells with AGPAT1
expressed in HEK-293 cells
expressed in HEK-293 or Sf9 insect cells
expressed in Saccharomyces cerevisiae strain INVSc1 and Nicotiana tabacum seeds
expression in C2C12 cell
expression in Chineses hamster ovary cells
-
expression in CHO cell, HeLa cell and HEK-293 cell
expression in Escherichia coli
expression in Escherichia coli JC201, a mutant strain deficient in the enzyme activity
-
expression in HEK-293 cell
expression in insect and mammalian cell lines. The enzyme gene is located in the MHC class III region
expression in JC201 Escherichia coli cells
-
expression in osteosarcoma cell
expression of a cDNA encoding protein with enzyme activity in transgenic plants. Expression in canola results in incorporation of laurate into the sn-2 position of triacylglycerol
-
expression of active His6-tagged CGI-58 in Escherichia coli
expression via recombinant adenoviruses in Agpat2 -/- mice
gene AGPAT2, expression of C-terminally HA-tagged AGPAT2 in CHO-K1 cells
gene AGPAT7, DNA and amino acid sequence determination and analysis, chromosomal mapping to 15q14, expression analysis
gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 17, genetic structure, expression analysis
-
gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 2p23.1, genetic structure, expression analysis, stable expression of wild-type and mutant enzymes in CHO cells
-
gene At4g24160, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene CHLREDRAFT_174358, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method
gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method, and seed-specific expression from Fp1::MaLPAAT with NptII selection marker, prepared by replacing the 35S promoter in above 35S::MaLPAAT construct with Brassica napus seed-specific Fp1 promoter. The DHA-producing transgenic Arabidopsis line (GA7) is supertransformed by Agrobacterium mediated floral dip method for MaLPAAT
-
gene LPAAT2, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method
gene LPAAT4, isolated from seed of unsaturated fatty acid (UFA)-rich Paeonia rockii, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of the enzyme in Arabidopsis thaliana resulting in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. AtDGAT1, AtGPAT9, and Atoleosin, involved in TAG assembly, are upregulated in PrLPAAT4-overexpressing lines. Recombinnat expression of GFP-tagged enzyme in tobacco lower epidermal cell plama membranes
KX256279
gene LPAT1, genetic structure, phylogenetic tree
gene LPAT2, genetic structure, LPAT2-cDNA driven by an LPAT2 promoter functionally complements insertion mutant lpat2 in transformed heterozygous mutants to produce the lpat2/lpat2 genotype, overview, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping
gene LPAT3, genetic structure, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping
gene LPAT4, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme
gene LPAT5, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme
gene PCC8801_1274, DNA and amino acid sequence determination and analysis, recombinant expression in Synechocystis sp. strain PCC 6803 resulting in and increase of the level of 14:0 from 2% to 16% of total fatty acids and decrease of 16:0 from 56% to 43%, analysis of the lipid composition, fatty acid composition of lipid classes and positional distribution of fatty acids, overview
gene plsC, functional recombinant expression in and complementation of the Escherichia coli temperature-sensitive mutant strain SM2-1 (plsC(Ts)), growth at 30°C. Chlamydia trachomatis PlsC also complements growth at 42°C. TmPlsC expression in strain SM2-1 results in predominately 16:0/16:0 and 18:1/16:0 phospholipids, indicating a TmPlsC substrate selectivity for 16:0. Overexpression of C- or N-terminally His6-tagged wild-type and selenomethionine-substituted enzymes in Escherichia coli
gene plsC, subcloning in Escherichia coli strain DH5alpha
-
gene plsC1, functional recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain C43(DE3), growth of recombinant cells at 18°C
gene plsC4, DNA and amino acid sequence determination and analysis, recombinant LI3-regulated expression of C-terminally His6-tagged PlsC4 in Escherichia coli strain S17-1/lambdapir
gene PrLPAAT1, DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant overexpression using a seed-specific promoter in Arabidopsis thaliana plants, recombinant expression of GFP-tagged enzyme in the lower epidermis of Nicotiana tabacum leaves by Agrobacterium-mediated transformation method
KX256278
gene Slc1, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method
genes BAT1.13 and BAT1.5, cloning of two cDNAs encoding microsomal isozymes from an immature embryo library, sequence comparisons, functional expression of the two rapeseed microsomal LPAAT isozymes in Arabidopsis thaliana, using Agrobacterium tumefaciens strain C58C1 transfection, leading to enhanced oil content and seed weight, tissue localization study, overview. Complementationof a thermosensitive Escherichia coli strain JC201 by expression of Bat1.5 and BAT1.13, overview
isolation and characterization of a gonococcal mutant
isolation and characterization of a meningococcal mutant, cloning of the meningococcal mutant gene
isolation of a cDNA encoding an enzyme by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape. Transformation of the acyltransferase-deficient Escherichia coli strain JC201 with the cDNA sequence BAT2 alleviates the temperature-sensitive phenotype of the plsC mutant and confers a palmitoyl-CoA-preferring enzyme activity to membrane fractions. Mapping of the BAT2 genes and sequence analysis of the BAT2 cDNA clone
-
isolation of a potential cDNA for the enzyme by heterologous screening with the maize pMAT1 clone, clone pLAT1
isolation of a potential cDNA for the enzyme by in vivo complementation of the Escherichia coli mutant JC201, clone pLAT2 is an enzyme clone
isolation of the JC201-plsC gene fragment containing the entire sequence of the enzyme. Complementation of the JC201 phenotype: transformation of JC201 cells with pplSC6 or pplsC6.3. Transformation of XL1-Blue cells with the control plasmid pplsC6.3, and the experimental plasmids pplsC6.3A or pplsC6.3L
-
LPAAT3, phylogenetic analysis, expression of the FLAG-tagged enzyme in CHO-K1 cells
C4B4E7
modification of the gene of Escherichia coli which encodes the enzyme by inserting an endoplasmic reticulum retrieval signal to its 3'end and introduccion into rapeseed plants under the control of a napin promotor
-
overexpressed in Escherichia coli BL21 (DE3), pCMV-SPORT6 vector, overexpressed in Saccharomyces cerevisiae, pYES2 vector
-
stable functional expression of GFP-tagged LPAAT3 in HeLa cells and in Rattus norvegicus hepatocytes with localization in the Golgi membranes
-
transformation of a modified plasmid into Escherichia coli JC201 enzyme-deficient strain and transformation of the plasmid DNA containing the cDNA BAT2 encoding the plastidial enzyme into the Escherichia coli XL-1 red mutator strain
transformation of plants of spring high-erocic acid rape, Hero, with a construct carrying the Limnanthes douglasii enzyme, LAT2, under control of the napin promoter. Study of the correlation between 22:1 accumulation at sn-2, production of trierucin and the expression of the transgene as detected by Western blot
-