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2.3.1.61: dihydrolipoyllysine-residue succinyltransferase

This is an abbreviated version!
For detailed information about dihydrolipoyllysine-residue succinyltransferase, go to the full flat file.

Word Map on EC 2.3.1.61

Reaction

succinyl-CoA
+
enzyme N6-(dihydrolipoyl)lysine
=
CoA
 +
enzyme N6-(S-succinyldihydrolipoyl)lysine

Synonyms

AceF, dihydrolipoamide acyltransacetylase, dihydrolipoamide S-succinyltransferase, dihydrolipoamide succinyltransferase, dihydrolipoic transsuccinylase, dihydrolipolyl succinyltransferase, dihydrolipolyl transsuccinylase, dihydrolipoyl succinyl transferase, dihydrolipoyl succinyltransferase, dihydrolipoyl transsuccinylase, DLST, DSCD, E2 component of alpha-ketoglutarate dehydrogenase complex, E2 subcomponent of the alpha-ketoglutarate dehydrogenase complex, E2o, KGD2, lipoate succinyltransferase, lipoic transsuccinylase, lipoyl transsuccinylase, OGDC-E2, SucB, succinyl-CoA:dihydrolipoate S-succinyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.61 dihydrolipoyllysine-residue succinyltransferase

Cloned

Cloned on EC 2.3.1.61 - dihydrolipoyllysine-residue succinyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding porcine E20 cloned, with expression vector pET-11d overexpressed in Escherichia coli
-
cDNA identification
cDNA, sequence analysis
-
cloning of the whole 2-oxoglutarase dehydrogenase complex structural genes, heterologous expression in Escherichia coli XL1-Blue and in the kgdA mutant Pseudomonas putida JS 347
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
-
expression in Escherichia coli
expression in Escherichia coli as a His-Nus A-tagged fusion protein
-
gene zdlst, DNA and amino acid sequence determination and analysis, genetic fine-mapping, and quantitative real-time PCR enzyme expression analysis
-
isolation of the gene and pseudogene for the dihydrolipoamide succinyltransferase component of the human 2-oxoglutarate dehydrogenase complex from a human genomic DNA library, cloning vector lambda EMBL3 subcloned into plasmid vector pUC18, gene is located on chromosome 14 at q24.2-q24.3, pseudogene is located on chromosome at p31
-
subcloning in Escherichia coli strain JM109
sucB gene cloned by immunological screening of a lambda EMBL3 genomic library from strain Nine Mile DNA, expressed in Escherichia coli
sucB gene encoding E2o cloned, sequenced and expressed in Escherichia coli