2.3.1.75: long-chain-alcohol O-fatty-acyltransferase

This is an abbreviated version!
For detailed information about long-chain-alcohol O-fatty-acyltransferase, go to the full flat file.

Reaction

Synonyms

acyl CoA wax alcohol acyltransferase 1, acyl CoA wax alcohol acyltransferase 2, acyl CoA:long-chain fatty alcohol O-acyltransferase, acyl-CoA wax alcohol acyltransferase, acyl-CoA wax alcohol acyltransferase 2, acyl-CoA wax alcohol acyltransferases, acyl-CoA:alcohol transacylase, acyl-CoA:fatty acyl alcohol acyltransferase, acyl-CoA:retinol acyltransferase, acyltransferase, long-chain alcohol, ArAT, ATF, ATF1, AtfA, AtfA1, AtfG25, AWAT, AWAT 1, AWAT 2, AWAT1, AWAT2, bifunctional WS/DGAT, EgWSD2, EgWSD5, fatty acyl-coenzyme A:fatty alcohol acyltransferase, isoprenoid wax ester synthase, Maqu_0168, MFAT, More, multifunctional O-acyltransferase, Rha1, TrWSD4, TrWSD5, unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase, wax ester synthase/acyl CoA:diacylglycerol acyltransferase, wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase/acyl-CoA diacylglycerol acyltransferase, wax ester synthase/acyl-CoA:diacylglycerol acyltransferase, wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase, wax ester synthase/diacylglycerol acyltransferase, wax ester synthases/acyl-CoA:diacylglycerol acyltransferase, wax monoester synthase, wax synthase, wax-ester synthase, waxester/diacylglycerol acyltransferase, WE synthase, WE synthases/acyl-CoA:diacylglycerol acyltransferase, WS, WS/DGAT, WS2, WSD1, WSD2, WSD4, WSD5

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.75 long-chain-alcohol O-fatty-acyltransferase

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Results	in table
6	Activating Compound
143	AA Sequence
11	Application
1	CAS Registry Number
47	Cloned(Commentary)
3	Cofactor
9	Disease/ Diagnostics
13	General Information
4	General Stability
10	Inhibitors
36	kcat/KM [mM/s]
1	Ki Value [mM]
48	KM Value [mM]
17	Localization
4	Metals/Ions
14	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
88	Organism
5	Pathway
1	PDB ID
14	pH Optimum
1	pH Range
9	pI Value
37	Protein Variants
9	Purification (Commentary)
3	Reaction
1	Reaction Type
69	Reference
39	Source Tissue
42	Specific Activity [micromol/min/mg]
3	Storage Stability
458	Substrates and Products (Substrate)
22	Subunits
158	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
7	Temperature Range [°C]
4	Temperature Stability [°C]
37	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.75 - long-chain-alcohol O-fatty-acyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

glutathione-Sepharose column chromatography and Superdex S200 gel filtration

Mus musculus

Q6E1M8

757376

HisTrap column chromatography and Superdex S200 gel filtration

Streptomyces sp. G25

-

755845

Ni2+-affinity column chromatography

Thraustochytrium roseum

A0A1Z2RWY4, A0A3G1ICG7

756245

partially

Simmondsia chinensis

Q9XGY6

487487

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography

Thraustochytrium roseum

A0A1Z2RWY4, A0A3G1ICG7

756245

recombinant enzyme from Escherichia coli by cation exchange chromatography, hydrophobic interaction chromatography and subsequent anion exchange chromatography or by means of a C-terminal His6 tag

Acinetobacter baylyi

-

672451

recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography

Streptomyces sp. G25

-

755845

recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography

Acinetobacter sp.

-

657612

recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange and hydrophobic interaction chromatography, followed by anion exchange chromatography

Acinetobacter baylyi

-

703547