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2.3.1.94: 6-deoxyerythronolide-B synthase

This is an abbreviated version!
For detailed information about 6-deoxyerythronolide-B synthase, go to the full flat file.

Word Map on EC 2.3.1.94

Reaction

propanoyl-CoA
+ 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ =
6-Deoxyerythronolide b
 + 7 CoA + 6 CO2 +
H2O
 + 6 NADP+

Synonyms

6-deoxyerythronolide B, 6-deoxyerythronolide B synthase, DEBS, DEBS1, DEBS2, DEBS3, deoxyerythronolide B synthase, deoxyerythronolide B synthase 1, deoxyerythronolide B synthase 2, deoxyerythronolide B synthase 3, eryA, erythromycin polyketide synthase, erythronolide condensing enzyme, erythronolide synthase, PKS, polyketide synthase, synthase, erythronolide

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.94 6-deoxyerythronolide-B synthase

Cloned

Cloned on EC 2.3.1.94 - 6-deoxyerythronolide-B synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a variant of DEBS1 is cloned into the vector pET21c
expression in Bacillus subtilis
expression in Escherichia coli
for expression in Escherichia coli cells
-
heterologous expression in Streptomyces coelicolor and Escherichia coli
-
the ACP construct is engineered with the FLAG epitope
-
the acyltransferase domains, AT1, AT2, AT4, AT5 and AT6, from DEBS are expressed as stand-alone proteins with their ketosynthase-to-acyltransferase and post-acyltransferase linkers, ketosynthase3-acyltransferase3 and ketosynthase6-acyltransferase6 didomains and discrete acyl carrier protein domain expression vectors are constructed, the vector pET28a is used for expression in Escherichia coli BL21DE3 cells
the thioesterase domain from deoxyerythronolide B synthase is cloned for expression in Escherichia coli cells
the three DEBS genes are sequentially integrated into the Escherichia coli BAP1 chromosome
-