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2.3.1.97: glycylpeptide N-tetradecanoyltransferase

This is an abbreviated version!
For detailed information about glycylpeptide N-tetradecanoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.97

Reaction

tetradecanoyl-CoA
+
an N-terminal-glycyl-[protein]
=
CoA
 +
an N-terminal-N-tetradecanoylglycyl-[protein]

Synonyms

CaNMT, cell division control protein 72, dipeptide N-myristoyltransferase 2, glycylpeptide N-tetradecanoyltransferase, glycylpeptide N-tetradecanoyltransferase 1, glycylpeptide N-tetradecanoyltransferase 2, HsNMT1, HsNMT2, LmNMT, More, myristoyl-CoA-protein N-myristoyltransferase, myristoyl-CoA: protein N-myristoyltransferase, myristoyl-CoA:protein N-myristoyltransferase, myristoyl-CoA:protein N-myristoyltransferase 1, myristoyl-CoA:protein N-myristoyltransferase 2, myristoyl-CoA:protein N-myristoyltransferase type 2, myristoyl-coenzyme A:protein N-myristoyl transferase, myristoyl-coenzyme A:protein N-myristoyltransferase, myristoylating enzymes, myristoyltransferase, myristoyltransferase type 1, myristoyltransferase, protein N-, N-myristoyl transferase, N-myristoyltransferase, N-myristoyltransferase 1, N-myristoyltransferase 2, NMT, NMT 1, NMT 2, NMT-1, NMT-2, NMT1, Nmt1p, NMT2, peptide N-myristoyltransferase, peptide N-myristoyltransferase 1, peptide N-myristoyltransferase 2, protein N-myristoyltransferase, PVX_085815, TbNMT, type I N-myristoyltransferase, type II N-myristoyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.97 glycylpeptide N-tetradecanoyltransferase

Cloned

Cloned on EC 2.3.1.97 - glycylpeptide N-tetradecanoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of cardiac muscle enzyme, expression in Escherichia coli, DNA and amino acid sequence determination
-
cloning of cDNA from liver mRNA, construction of 2 expression plasmids with ATG at position 28 and 211, respectively, termed h28NMT and h211NMT, functional expression in Escherichia coli and h28NMT in HEK 293 cells
-
codon-optimized Nef plasmid and the human NMT plasmid were cotransformed in BL21(DE3)
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construction of expression plasmid encoding the enzyme, lacking the first 8 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, expression in Escherichia coli DH5alpha, cDNA from spleen
-
construction of expression plasmid encoding the enzyme, lacking the first 9 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, overexpression in Escherichia coli DH5alpha
-
DNA and amino acid sequence determination and analysis, overexpression of the His-tagged NMT in Escherichia coli strain BL21(DE3) pRareS
expression as His-tagged protein in Escherichia coli
-
expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli JM101, coexpression of each of the 4 homologous rat alpha subunits of the signal-transducing, heterotrimeric G proteins to determine in vivo interaction of the 2 enzymes, structural analysis
-
expression in Escherichia coli strain BL21
-
expression in Escherichia coli, DNA sequence determination
-
expression of His-tagged wild-type and mutants in Escherichia coli
-
expression of mutant nmt1-181 in Escherichia coli
-
expression of wild-type and mutants from Escherichia coli DH5alpha
-
expression of wild-type and mutants in Escherichia coli
-
functional expression in Escherichia coli as His-tagged protein, DNA sequence analysis
gene cloned from cell culture and lymphocytes, in vitro translation of 2 different splice variants, DNA and amino acid sequence determination
-
NMT2, DNA and amino acid sequence determination, analysis, and comparisons, phylogenetic tree, expression of His6-tagged NMT2 in Escherichia coli
overexpression in COS-7 cell, isoform 1
overexpression in COS-7 cell, isoform 1 and 2
overexpression in COS-7 cell, isoform 2
overexpression in Escherichia coli strain BL21(DE3)
recombinant expression of His6-tagged Nef:NMT complex in Escherichia coli Codon Plus (DE-)-RIL cells
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sequence comparisons