2.3.2.22: cyclo(L-leucyl-L-leucyl) synthase
This is an abbreviated version!
For detailed information about cyclo(L-leucyl-L-leucyl) synthase, go to the full flat file.
Reaction
2 L-leucyl-tRNALeu = 2 tRNALeu +
Synonyms
CDPS, cLL synthase, cyclo(L-leucyl-L-leucyl) synthase, cyclodileucine synthase, cyclodipeptide synthase, pSHaeC06, Shae-CDPS, YvmC
ECTree
Advanced search results
Crystallization
Crystallization on EC 2.3.2.22 - cyclo(L-leucyl-L-leucyl) synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
in the apo form and complexed with substrate mimics, at 1.7-2.4 A resolutions. Data show the presence of an aminoacyl-enzyme reaction intermediate, but not a dipeptide tRNA intermediate. Reaction follows a sequential catalytic mechanism, with the successive attachment of two leucine residues on the enzyme via a conserved serine residue
purified recombinant enzyme, crystallization from 30% PEG 4000, 0.1 M Tris-HCl, pH 8.5, and 0.2 M lithium sulfate monohydrate, X-ray diffraction structure determination and analysis at 2.99 A resolution