Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.2.3: lysyltransferase

This is an abbreviated version!
For detailed information about lysyltransferase, go to the full flat file.

Word Map on EC 2.3.2.3

Reaction

L-lysyl-tRNALys
+
phosphatidylglycerol
=
tRNALys
 +
3-O-L-lysyl-1-O-phosphatidylglycerol

Synonyms

LPG synthetase, Lys-tRNA(Lys) phosphatidylglycerol transferase, lysophosphatidylglycerol synthetase, LysX, LysX protein, lysyl-PG synthase, lysyl-transferase-lysyl-tRNA synthetase, lysyltransferase, MprF, MprF flippase, MprF protein, mprF-lysU protein, MprF2, multiple peptide resistance factor protein, phosphatidylglycerol lysyltransferase, phospholipid flippase, RimK-related lysine biosynthesis protein, Rv1640c, Tk0278, TkLysX

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.3 lysyltransferase

Engineering

Engineering on EC 2.3.2.3 - lysyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I701T
D546A
MprF(-8) mutant, replacement results in slightly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein
D731A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
E624A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
E685A
MprF(-8) mutant, replacement results in strongly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein
I420N
-
naturally occuring single nucleotide polymorphism (SNP) that does not alter daptomycin resistance and the lysyl-phosphatidylglycerol synthesizing activity
K547A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
K621A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
K806A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
L826F
-
naturally occuring single nucleotide polymorphism (SNP) that does not alter daptomycin resistance and the lysyl-phosphatidylglycerol synthesizing activity
MprF(-10)
mutant, truncated protein consisting of residues 363-840
MprF(-12)
mutant, truncated protein consisting of residues 437-840
MprF(-14)
mutant, truncated protein consisting of residues 510-840
MprF(-2)
mutant, truncated protein consisting of residues 84-840
MprF(-4)
mutant, truncated protein consisting of residues 157-840
MprF(-6)
mutant, truncated protein consisting of residues 219-840
MprF(-8)
mutant, truncated protein consisting of residues 274-840
MprF(-C)
mutant, truncated protein consisting of residues 1-586
P314L
-
naturally occuring single nucleotide polymorphism (SNP) that does not alter daptomycin resistance, but slightly enhances the lysyl-phosphatidylglycerol synthesizing activity
R734A
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
S295L
-
naturally occuring single nucleotide polymorphism (SNP) that causes a slight daptomycin resistance and slightly enhances the lysyl-phosphatidylglycerol synthesizing activity
S337L
-
naturally occuring single nucleotide polymorphism (SNP) that does not alter daptomycin resistance and the lysyl-phosphatidylglycerol synthesizing activity
T345A
-
naturally occuring single nucleotide polymorphism (SNP) that can reproducibly cause strong, clinically relevant daptomycin resistance, the mutation leads to weakened intramolecular domain interactions of MprF, suggesting that daptomycin and friulimicin resistance-conferring mutations may alter the substrate range of the MprF flippase to directly translocate these lipopeptide antibiotics or other membrane components with crucial roles in the activity of these antimicrobials. MprF point mutation T345A causes cross-resistance only to daptomycin and the related lipopeptide antibiotic friulimicin B
V351E
-
naturally occuring single nucleotide polymorphism (SNP) that causes a strong, clinically relevant daptomycin resistance and slightly reduced lysyl-phosphatidylglycerol synthesizing activity
additional information