2.3.2.B12: ubiquitin transferase U-box E4
This is an abbreviated version!
For detailed information about ubiquitin transferase U-box E4, go to the full flat file.
Word Map on EC 2.3.2.B12
Reaction
Synonyms
CHIP, CYC4, DUF1076 domain-containing protein, E4, E4 factor, E4 ligase, E4 ubiquitin ligase, E4 ubiquitin-protein ligase UFD2, MUSE3, PRP19, StoD, STY1076, Ub-conjugating E4 factor, Ube4A, UBE4B, ubiquitin chain elongation enzyme Ufd2, ubiquitin conjugation factor E4, ubiquitin fusion degradation protein 2, Ubr1-dependent E4 enzyme, UFD-2, Ufd2, Ufd2a, Ufd2b, Ufd4, Ufd4-enhancing ubiquitiylation enzyme
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2.3.2.B12 ubiquitin transferase U-box E4Advanced search results
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results (Substrates Products
Substrates Products on EC 2.3.2.B12 - ubiquitin transferase U-box E4
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[U-box-E4-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
[U-box-E4-ubiquitin-carrier protein]-L-cysteine + N6-[N6-ubiquitinyl-L-lysyl]-ubiquitinyl-[acceptor protein]-L-lysine
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?
[CHIP U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[CHIP U-box-E4-ubiquitin-carrier protein Ubc4]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CHIP is greatest with E2 enzymes Ubc4 or UbcH5C
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?
[CHIP U-box-E4-ubiquitin-carrier protein UbH5C4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[CHIP U-box-E4-ubiquitin-carrier protein UbcH5C]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CHIP is greatest with E2 enzymes Ubc4 or UbcH5C
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?
[CYC4 U-box-E4-ubiquitin-carrier protein Ubc2B]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[CYC4 U-box-E4-ubiquitin-carrier protein Ubc2B]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
HSU37219, XM006045
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2B or Ubc3, but is also activ with UbcH7
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?
[CYC4 U-box-E4-ubiquitin-carrier protein Ubc3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[CYC4 U-box-E4-ubiquitin-carrier protein Ubc3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
HSU37219, XM006045
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2 or Ubc3, but is also activ with UbcH7
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?
[CYC4 U-box-E4-ubiquitin-carrier protein UbcH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[CYC4 U-box-E4-ubiquitin-carrier protein UbcH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
HSU37219, XM006045
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2 or Ubc3, but is also activ with UbcH7
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?
[p300/CREB-binding protein U-box-E4-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [acceptor p53]-N6-ubiquitinyl-L-lysine
[p300/CREB-binding protein U-box-E4-ubiquitin-carrier protein]-L-cysteine + [acceptor p53]-N6-ubiquitinyl-ubiquitinyl-L-lysine
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both p300 and CREB-binding protein are required for endogenous p53 polyubiquitination and the normally rapid turnover of p53 in unstressed cells. CREB-binding protein deficiency specifically stabilizes cytoplasmic, but not nuclear p53. The N-terminal 616 aa of CREB-binding protein, which includes the conserved Zn2+-binding C/H1-TAZ1 domain, is the minimal domain sufficient to destabilize p53 in vivo
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?
[PRP19 U-box-E4-ubiquitin-carrier protein Ubc3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[PRP19 U-box-E4-ubiquitin-carrier protein Ubc3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
HSU37219, XM006045
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of PRP19 is greatest with E2 enzyme Ubc3
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?
[UFD2a U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[U-box-E4-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2a is greatest with E2 enzymes Ubc4 or UbcH5C
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?
[UFD2b U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[UFD2b U-box-E4-ubiquitin-carrier protein Ubc4]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2b is greatest with E2 enzymes Ubc4 or UbcH5C
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?
[UFD2b U-box-E4-ubiquitin-carrier protein UbH5C]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
[UFD2b U-box-E4-ubiquitin-carrier protein UbcH5C]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2b is greatest with E2 enzymes Ubc4 or UbcH5C
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?
additional information
?
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MUSE3 functions as an E4 factor and works downstream of the E3 ligase SCFCPR1 to facilitate the polyubiquitination and degradation of Resistance proteins, including SNC1 (Supressor of NPR1, Constitutive 1) and RPS2 (Resistance to Pseudomonas syringae 2)
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?
additional information
?
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HSU37219
data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3
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?
additional information
?
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XM006045
data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3
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?
additional information
?
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AI390103
data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3
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?
additional information
?
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data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3
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?
additional information
?
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data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3
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?
additional information
?
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the U-box domain is essentiel for the involvement of Ufd2 in Pex29 degradation. Pex29 is a peroxiisomal membrane protein that regulates the number and size of peroxisomes. Pex29 is regulated by ubiquitin-protein E3 ligase. Further extension of the growing ubiquitin chain requires assistance of Ufd2 due to the spatial positioning of the E3 and its substrate. Ufd2 elongates Pex29 with predominatly Lys48-linked ubiquitin chain in vivo
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?
additional information
?
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one target of Ufd2 is identified as Pex29, the ubiquitin-protein conjugate ubiquitinylated by the ubiquitin-protein E3 ligase Doa10. Not all Doa10 substrates are regulated by Ufd2, suggesting that E4 involvement is not specific to a particular E3, but might depend on the spatial arrangement of the E3-substrate interaction
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?
additional information
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Ufd4-enhancing ubiquitinylation of N-end rule substrates, Lys48 ubiquitin-chain formation and proteasomal degradation
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?
additional information
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the U-box domain is essentiel for the involvement of Ufd2 in Pex29 degradation. Pex29 is a peroxiisomal membrane protein that regulates the number and size of peroxisomes. Pex29 is regulated by ubiquitin-protein E3 ligase. Further extension of the growing ubiquitin chain requires assistance of Ufd2 due to the spatial positioning of the E3 and its substrate. Ufd2 elongates Pex29 with predominatly Lys48-linked ubiquitin chain in vivo
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?
additional information
?
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one target of Ufd2 is identified as Pex29, the ubiquitin-protein conjugate ubiquitinylated by the ubiquitin-protein E3 ligase Doa10. Not all Doa10 substrates are regulated by Ufd2, suggesting that E4 involvement is not specific to a particular E3, but might depend on the spatial arrangement of the E3-substrate interaction
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?
additional information
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the StoD C terminus is a U-box E3 ubiquitin ligase, capable of autoubiquitination in the presence of multiple E2 enzymes
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