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2.3.3.10: hydroxymethylglutaryl-CoA synthase

This is an abbreviated version!
For detailed information about hydroxymethylglutaryl-CoA synthase, go to the full flat file.

Word Map on EC 2.3.3.10

Reaction

acetyl-CoA
+
H2O
 +
acetoacetyl-CoA
=
(S)-3-hydroxy-3-methylglutaryl-CoA
 +
CoA

Synonyms

(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating), 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase, 3-hydroxy-3-methylglutaryl CoA synthase, 3-hydroxy-3-methylglutaryl CoA synthase I, 3-hydroxy-3-methylglutaryl CoA synthetase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl-CoA synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 1, 3-hydroxy-3-methylglutaryl-CoA synthase 2, 3-hydroxy-3-methylglutaryl-coenzyme A synthase, 3-hydroxy-3-methylglutaryl_coenzyme A synthase, 3-hydroxyl-3-methyl-glutaryl-CoA synthase, acetoacetyl coenzyme A transacetase, beta-hydroxy-beta-methylglutaryl-CoA synthase, BjHMGS1, BjHMGS2, BjHMGS3, BjHMGS4, EC 4.1.3.5, GbHMGS2, GhHMGS1A, GhHMGS1D, GhHMGS2D, GhHMGS3A, GhHMGS3D, HGMS, HMG-CoA, HMG-CoA synthase, HMG-CoA synthase 1, HMG-CoS synthase, HMGCS, HMGCS1, HMGCS2, HMGS, HMGS-2, HMGS1, HMGS2, hydroxy-methylglutaryl coenzyme-A synthase, hydroxymethylglutaryl CoA synthase 2, hydroxymethylglutaryl CoA synthetase, hydroxymethylglutaryl coenzyme A synthase, hydroxymethylglutaryl coenzyme A-condensing enzyme, hydroxymethylglutaryl-CoA synthase, hydroxymethylglutaryl-coenzyme A synthase, LcHMGS, mvaS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.10 hydroxymethylglutaryl-CoA synthase

Cloned

Cloned on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 cytosolic HMG-CoA synthase genes, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S
-
cDNA cloned and sequenced
cDNA cloned by complementation of a bap1 mutation and expressed in Saccharomyces cerevisiae
-
cDNA subcloned and expressed from a T7-based vector in Escherichia coli
-
cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway
-
expressed in 3-hydroxy-3-methylglutaryl-CoA synthase knockout yeast strain YSC6274
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIPL cells
-
expressed in Nicotiana tabacum cultivar Xanthi
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli strain DH5a
expression in Haloferax volcanii
expression of recombinant wild-type cytoplasmic and Cys129 mutant enzymes
-
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3)
His6-tagged protein from Escherichia coli,His6-BjHMGS1
-
HMGS1, expression in Escherichia coli
-
into the vector pET-HSyn for expression in Escherichia coli cells
-
intronless gene, encodes for an active enzyme able to complement eukaryotic Mev-1 cells
-
mvaS gene isolated, expressed in Escherichia coli from a pET28 vector
-
pig mitochondrial HMG-CoA synthase encodes an active enzyme in the eukaryotic cell line Mev-1 and in Escherichia coli BL21 (DE3)
-
rat mitochondrial HMG-CoA synthase encodes an active enzyme in the eukaryotic cell line Mev-1 in Escherichia coli BL21 (DE3)
-