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2.3.3.13: 2-isopropylmalate synthase

This is an abbreviated version!
For detailed information about 2-isopropylmalate synthase, go to the full flat file.

Word Map on EC 2.3.3.13

Reaction

acetyl-CoA
+
3-methyl-2-oxobutanoate
+
H2O
=
(2S)-2-isopropylmalate
+
CoA

Synonyms

2-isopropylmalate synthase 1, chloroplastic, 2-isopropylmalate synthase 2, chloroplastic, 3-hydroxy-4-methyl-3-carboxy-pentanoate 2-oxo-3-methyl-butanoate lyase (CoA-acetylating), 3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methyl-butyrate lyase (CoA-acetylating), alpha-IPM synthase, Alpha-IPM synthetase, alpha-IPMS, alpha-IPMS-14CR, alpha-IPMS-2CR, Alpha-isopropylmalate synthase, alpha-isopropylmalate synthase I, alpha-isopropylmalate synthase II, alpha-isopropylmalate synthetase, alpha-isopropylmalic synthetase, BatIMS, EC 4.1.3.12, IPM synthase, IPM-synthase, IPMS, IPMS1, IPMS2, IPMS3, isopropylmalate synthase, isopropylmalate synthase 1, isopropylmalate synthetase, LEU4, LEU9, leuA, Mj1195, MtalphaIPMS, MtIPMS, NmeIPMS, Os11g04670, Os12g04440, OsIPMS1, OsIPMS2, ROL17, synthase, 2-isopropylmalate, synthase, alpha-isopropylmalate, TT_C0849

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.13 2-isopropylmalate synthase

Crystallization

Crystallization on EC 2.3.3.13 - 2-isopropylmalate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.8 mM sodium formate, and 30% (w/v) PEG 2000 monomethyl ether
sitting drop vapor diffusion method
-
truncated protein LeuA425, sitting drop vapor diffusion method, using 24% (w/v) PEG 3350 and 275 mM lithium nitrate
truncated protein E365Term, sitting drop vapor diffusion method, using 0.2 M magnesium acetate and 18% (w/v) mPEG 3350
comparison of wild-type and mutant D578Y structures by homology modelling. D578 is important for leucine feedback inhibition
-