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2.3.3.14: homocitrate synthase

This is an abbreviated version!
For detailed information about homocitrate synthase, go to the full flat file.

Word Map on EC 2.3.3.14

Reaction

acetyl-CoA
+
H2O
 +
2-oxoglutarate
=
(2R)-2-hydroxybutane-1,2,4-tricarboxylate
 +
CoA

Synonyms

2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase (CoA-acetylating), acetyl-coenzyme A: 2-ketoglutarate C-transferase, acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase, EC 4.1.3.21, HCS, HcsA, homocitrate synthase, homocitrate synthetase, homocitrate-condensing enzyme, homocondensing enzyme, LYS20, LYS21, Lys21p, LYS22, Lys22p, nifV, nifV2, saci_1304, SpHCS, synthase, homocitrate, TtHCS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.14 homocitrate synthase

Cloned

Cloned on EC 2.3.3.14 - homocitrate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed at high levels in Escherichia coli
-
expressed in Escherichia coli B834 DE3 cells
-
expressed in Escherichia coli BL21(DE3) RIL star cells
-
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells
expressed in Escherichia coli Rosetta (DE3) pLysS cells
-
expression in Escherichia coli
expression in Escherichia coli. Introduction of homocitrate synthase, homoaconitase and homoisocitrate dehydrogenase from Saccharomyces cerevisiae into Escherichia coli enables +1 carbon extension from 2-oxoglutarate to 2-oxoadipate, which is subsequently converted into glutarate by a promiscuous 2-oxo acid decarboxylase (KivD) and a succinate semialdehyde dehydrogenase (GabD)
into the parallel expression vector pHIS2
-
overexpression in Penicillium chrysogenum using additional copies of lys1 with its own promoter or under the control of the pcbC promoter in either autonomously replicating or integrative vectors. Tranformants containing 3 to 32 additional copies of the lys1 gene are selected
-
three expression systems are designed, TtHCS without tag, with a C-terminal His6 tag and with a N-terminal His8 tag, the vectors pBluescript II SK+ and pET26b+ are used for expression in Escherichia coli cells
-