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2.3.3.16: citrate synthase (unknown stereospecificity)

This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.

Word Map on EC 2.3.3.16

Reaction

acetyl-CoA
+
H2O
 +
oxaloacetate
=
citrate
 +
CoA

Synonyms

bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.16 citrate synthase (unknown stereospecificity)

Cloned

Cloned on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alfalfa is engineered by introducing the citrate synthase gene controlled by the Arabidopsis Act2 constitutive promoter or the tobacco RB7 root-specific promoter. Fifteen transgenic plants are assayed for exclusion of aluminum from the root tip, for internal citrate content, for growth in in vitro assays, or for shoot and root growth in either hydroponics or in soil assays. Based on the soil assays, two transgenic events are identified that are more aluminum-tolerant than the non-transgenic control, confirming that citrate synthase overexpression can be a useful tool to help achieve aluminum tolerance
-
cloning and expression in Aspergillus nidulans, DNA sequence
cloning and expression of citA in Aspergillus niger, 11fold overexpression does not increase the citrate activity level in vivo
cloning and expression of wild-type and mutants in Escherichia coli
-
cloning and sequencing
cloning of His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase, expression in Escherichia coli
-
codon-optimized expression in Escherichia coli
ectopic overexpression in Arabidopsis thaliana strain WS via infection with Agrobacterium tumefaciens, DNA sequence determination
expressed in Arabidopsis thaliana
-
expressed in Bacillus subtilis
-
expressed in Corynebacterium glutamicum strain ATCC 13032
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(lambdaDE3) cells
expressed in Escherichia coli C41(DE3) cells
-
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Hms174(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. The inactive enzyme is reactivated following overnight incubation in 2 M KCl
expressed in Nicotiana benthamiana
expressed in Pichia pastoris, expressed in Nicotiana tabacum
-
expressed in Pseudomonas fluorescens ATCC 13525
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli with polyhistidine tags
expression of a chimeric protein with one Acinetobacter domain in Escherichia coli, domain interactions, subunit interactions
-
expression of a chimeric protein with one Escherichia coli domain in Escherichia coli, domain interactions, subunit interactions
-
expression of chimeric mutant in Escherichia coli citrate synthase deficient strain MOB154
-
expression of labeled mitochondrial isozyme in Saccharomyces from plasmid, NMR measurements in vivo
-
expression of wild-type and mutant in Escherichia coli
-
expression of wild-type, chimeric mutant and site-directed mutants in Escherichia coli citrate synthase deficient strain MOB154
-
functional expression in citrate synthase deficient Escherichia coli mutant strain K214 under control of lacZ promotor, DNA and amino acid sequence analysis
-
functional expression of peroxisomal isozyme CS II in citrate synthase deficient Escherichia coli mutant and wild-type Escherichia coli strain
-
in vitro translation of glyoxysomal isozyme, heterologous expression in Xenopus laevis oocytes
-
overexpression in Escherichia coli
overexpression in Escherichia coli JM105, DNA and amino acid sequence analysis
-
overexpression of the CS gene under control of the Arabidopsis thaliana Act2 constitutive promoter or the Nicotiana tabacum RB7 root-specific promoter in alfalfa, Medicago sativa, using the Agrobacterium tumefaciens, strain LBA4404, transformation method
-
the 1195-bp fragment of the mitochondrial citrate synthase enzyme is cloned in antisense orientation into the vector pBINAR between the CaMV 35S promoter and the ocs terminator. This construct is introduced into plants by an Agrobacterium
-