Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.002 - 90.3
oxaloacetate
0.0017 - 0.016
propionyl-CoA
additional information
additional information
-
0.00067
acetyl-CoA
-
wild-type, pH 8.0, 20°C
0.00085
acetyl-CoA
-
mutant W348Y, pH 8.0, 20°C
0.0017
acetyl-CoA
-
recombinant enzyme
0.0025
acetyl-CoA
methylcitrate synthase exhibiting also citrate synthase activity
0.003
acetyl-CoA
-
pH and temperature not specified in the publication
0.0036
acetyl-CoA
-
mutant W245F/W115F/W17F, pH 8.0, 20°C
0.004
acetyl-CoA
-
wild-type
0.004
acetyl-CoA
-
mitochondrial isozyme CS I
0.0045
acetyl-CoA
-
recombinant mutant G196V
0.005
acetyl-CoA
-
wild-type
0.005
acetyl-CoA
-
recombinant wild-type
0.007
acetyl-CoA
-
dimeric enzyme form
0.0075
acetyl-CoA
pH and temperature not specified in the publication
0.0075
acetyl-CoA
25°C, pH and temperature not specified in the publication
0.01
acetyl-CoA
-
mutant H187Q, pH 8.0, 20°C
0.011
acetyl-CoA
-
recombinant wild-type and mutant S43C
0.011
acetyl-CoA
-
native peroxisomal isozyme CS II
0.0116
acetyl-CoA
pH 8.0, 25°C
0.012
acetyl-CoA
-
dimeric enzyme form
0.0123
acetyl-CoA
30°C, pH 8, recombinant enzyme
0.013 - 0.014
acetyl-CoA
-
recombinant peroxisomal isozyme CS II
0.014
acetyl-CoA
-
with 0.1 M KCl
0.0141
acetyl-CoA
30°C, pH 8, native enzyme
0.0165
acetyl-CoA
pH 8.0, 25°C
0.017
acetyl-CoA
-
mutant R344K, pH 8.0, 20°C
0.018
acetyl-CoA
pH 8.0, 25°C
0.032
acetyl-CoA
-
K283 acetylated variant, pH 7.8, 25°C
0.037
acetyl-CoA
-
mutant A361R/A10E
0.038
acetyl-CoA
-
mutant A361R
0.049
acetyl-CoA
-
mutant enzyme H110A, in presence of 0.1 M KCl
0.05
acetyl-CoA
-
in presence of 2.5 mM ATP
0.05
acetyl-CoA
-
isoenzyme CSII
0.056
acetyl-CoA
-
mutant enzyme Q182A, in presence of 0.1 M KCl
0.0584
acetyl-CoA
at pH 8.0 and 35°C
0.069
acetyl-CoA
-
mutant enzyme N189A, in presence of 0.1 M KCl
0.069
acetyl-CoA
-
mutant enzyme R109L, in presence of 0.1 M KCl
0.076
acetyl-CoA
pH 7.5, 37°C
0.079
acetyl-CoA
-
mutant enzyme T111A, in presence of 0.1 M KCl
0.08
acetyl-CoA
-
in presence of 5 mM ATP
0.08
acetyl-CoA
-
mutant enzyme R163L, in presence of 0.1 M KCl
0.088
acetyl-CoA
-
in presence of 100 mM KCl
0.089
acetyl-CoA
pH not specified in the publication, temperature not specified in the publication
0.098
acetyl-CoA
-
wild-type, pH 7.8, 25°C
0.11
acetyl-CoA
-
wild-type
0.12
acetyl-CoA
-
wild-type
0.12
acetyl-CoA
-
wild-type enzyme, in presence of 0.1 M KCl
0.143 - 0.161
acetyl-CoA
-
strains X and Hanham
0.145
acetyl-CoA
-
K168 acetylated variant, pH 7.8, 25°C
0.153
acetyl-CoA
presence of 5 mM ADP, pH 7.5, 37°C
0.174
acetyl-CoA
-
in absence of KCl
0.194
acetyl-CoA
presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C
0.2
acetyl-CoA
-
wild-type
0.21
acetyl-CoA
-
mutant enzyme T204A, in presence of 0.1 M KCl
0.22
acetyl-CoA
pH 7.5, 37°C
0.22
acetyl-CoA
-
mutant enzyme K167A, in presence of 0.1 M KCl
0.23
acetyl-CoA
-
mutant enzyme Y145A, in presence of 0.1 M KCl
0.26
acetyl-CoA
-
minimum at 1 M KCl
0.478
acetyl-CoA
-
K295 acetylated variant, pH 7.8, 25°C
0.69
acetyl-CoA
mutant enzyme G181E, pH and temperature not specified in the publication
0.7
acetyl-CoA
-
wild-type
0.75
acetyl-CoA
wild type enzyme, pH and temperature not specified in the publication
0.86
acetyl-CoA
-
isoenzyme CSI
1.01
acetyl-CoA
mutant enzyme T204R, pH and temperature not specified in the publication
1.24
acetyl-CoA
-
mutant A10E
71.6
acetyl-CoA
at pH 8.0 and 20°C
123
acetyl-CoA
30°C, pH 8.0, recombinant enzyme
125
acetyl-CoA
30°C, pH 8.0, native enzyme
0.0005
citrate
-
mutant H187Q, pH 8.0, 20°C
0.0005
citrate
-
mutant W348Y, pH 8.0, 20°C
0.0007
citrate
-
mutant W245F/W115F/W17F, pH 8.0, 20°C
0.001
citrate
-
wild-type, pH 8.0, 20°C
0.002
oxaloacetate
-
wild-type
0.002
oxaloacetate
-
mutant A361R
0.002
oxaloacetate
-
CS II
0.002
oxaloacetate
-
dimeric enzyme form with propionyl-CoA
0.002
oxaloacetate
-
isoenzyme CSII
0.003
oxaloacetate
-
mitochondrial isozyme CS I
0.003
oxaloacetate
-
dimeric enzyme form with acetyl-CoA
0.003
oxaloacetate
-
mutant enzyme H110A, in presence of 0.1 M KCl
0.003
oxaloacetate
-
mutant W245F/W115F/W17F, pH 8.0, 20°C
0.003
oxaloacetate
-
mutant W348Y, pH 8.0, 20°C
0.003
oxaloacetate
-
wild-type, pH 8.0, 20°C
0.004
oxaloacetate
-
mutant enzyme T204A, in presence of 0.1 M KCl
0.0043
oxaloacetate
30°C, pH 8, native enzyme
0.005
oxaloacetate
-
recombinant enzyme
0.005
oxaloacetate
-
mutant enzyme R163L, in presence of 0.1 M KCl
0.0052
oxaloacetate
-
pH and temperature not specified in the publication
0.0057
oxaloacetate
-
recombinant mutant G196V
0.006
oxaloacetate
-
mutant A361R/A10E
0.006
oxaloacetate
-
hexameric enzyme form
0.006 - 0.007
oxaloacetate
-
native and recombinant peroxisomal isozyme CS II
0.0066
oxaloacetate
-
recombinant wild-type
0.007
oxaloacetate
-
wild-type
0.007
oxaloacetate
pH 7.5
0.007
oxaloacetate
-
mutant enzyme R109L, in presence of 0.1 M KCl
0.008
oxaloacetate
-
mutant A10E
0.0081
oxaloacetate
30°C, pH 8, recombinant enzyme
0.01
oxaloacetate
-
recombinant wild-type and mutant S34C
0.01
oxaloacetate
-
mutant enzyme Q182A, in presence of 0.1 M KCl
0.0105
oxaloacetate
mutant enzyme G181E, pH and temperature not specified in the publication
0.011
oxaloacetate
-
wild-type
0.011
oxaloacetate
-
in presence of KCl
0.011
oxaloacetate
wild type enzyme, pH and temperature not specified in the publication
0.0112
oxaloacetate
at pH 8.0 and 35°C
0.015
oxaloacetate
-
wild-type
0.015
oxaloacetate
-
mutant A361R
0.017
oxaloacetate
-
mutant enzyme N189A, in presence of 0.1 M KCl
0.018
oxaloacetate
-
minimum at 1 M KCl
0.018
oxaloacetate
-
mutant enzyme T111A, in presence of 0.1 M KCl
0.0183
oxaloacetate
pH and temperature not specified in the publication
0.0183
oxaloacetate
25°C, pH and temperature not specified in the publication
0.02
oxaloacetate
-
wild-type
0.02
oxaloacetate
-
mutant mutant loop/K313L/A361R
0.021
oxaloacetate
-
wild-type, pH 7.8, 25°C
0.023
oxaloacetate
-
K283 acetylated variant, pH 7.8, 25°C
0.025
oxaloacetate
-
K168 acetylated variant, pH 7.8, 25°C
0.026
oxaloacetate
-
wild-type
0.026
oxaloacetate
-
wild-type enzyme, in presence of 0.1 M KCl
0.026
oxaloacetate
30°C, pH 8.0, native enzyme
0.027
oxaloacetate
30°C, pH 8.0, recombinant enzyme
0.032
oxaloacetate
pH not specified in the publication, temperature not specified in the publication
0.032
oxaloacetate
-
K295 acetylated variant, pH 7.8, 25°C
0.037
oxaloacetate
-
mutant enzyme K167A, in presence of 0.1 M KCl
0.0414
oxaloacetate
pH 8.0, 25°C
0.05
oxaloacetate
-
CS II
0.051
oxaloacetate
-
mutant enzyme Y145A, in presence of 0.1 M KCl
0.054
oxaloacetate
-
mutant H187Q, pH 8.0, 20°C
0.073
oxaloacetate
presence of 5 mM ADP, pH 7.5, 37°C
0.091
oxaloacetate
pH 7.5, 37°C
0.103
oxaloacetate
pH 8.0, 25°C
0.108
oxaloacetate
presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C
0.138
oxaloacetate
pH 8.0, 25°C
0.22
oxaloacetate
at pH 8.1 and 28°C
0.37
oxaloacetate
-
mutant R344K, pH 8.0, 20°C
0.63
oxaloacetate
with propionyl-CoA, methylcitrate synthase exhibiting also citrate synthase activity
0.96
oxaloacetate
-
isoenzyme CSI
90.3
oxaloacetate
at pH 8.0 and 20°C
0.0017
propionyl-CoA
methylcitrate synthase exhibiting also citrate synthase activity
0.003
propionyl-CoA
-
dimeric enzyme form
0.004
propionyl-CoA
-
mutant A361R
0.008
propionyl-CoA
-
mutant loop/K313L/A361R
0.016
propionyl-CoA
-
wild-type
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km values for acetyl-CoA and oxaloacetate of mutants with a loop introduced into the active site
-
additional information
additional information
-
mutants, with and without KCl, overview
-
additional information
additional information
-
Km increases 5fold with salt concentration from 1 to 3 M, overview
-
additional information
additional information
-
influence of NADH and AMP on Km for acetyl-CoA
-
additional information
additional information
-
affinity for oxaloacetate is increased at adaption of growth temperature from 5 to 15°C
-
additional information
additional information
-
allosteric, Hill coefficient: 1.5
-
additional information
additional information
no difference in the apparent Km-value of oxaloacetate at any temperature, indicating that the substrate affinity of citrate synthase shows no adaption to changes in thermal environment
-
additional information
additional information
no difference in the apparent Km-value of oxaloacetate at any temperature, indicating that the substrate affinity of citrate synthase shows no adaption to changes in thermal environment
-