2.3.3.16: citrate synthase (unknown stereospecificity)
This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.
Reaction
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Synonyms
bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase
ECTree
Purification
Purification on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)
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heat step and dye-ligand affinity chromatography
HisTrap column chromatography and Source 15Q column chromatography
Matrex Red Gel A affinity column chromatography
mitochondrial enzyme, antibody studies
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mitochondrial isozyme partially, glyoxysomal isozyme to homogeneity
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native and recombinant wild-type, the latter from E. coli and recombinant S43C mutant enzyme from E. coli
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Ni-NTA column chromatography
Ni-NTA column chromatography, Superdex 200 gel filtration, and Sephadex G25 gel filtration
Ni2+-loaded iminodiacetic acid Sepharose column chromatography and Sephadex 200 gel filtration
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nickel-affinity column chromatography
recombinant chimeric mutants from E. coli
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recombinant chimeric protein from E. coli
recombinant enzyme from E. coli
recombinant from E. coli
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recombinant His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase from E. coli
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recombinant peroxisomal isozyme CS II from E. coli
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recombinant protein expressed from plasmid
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recombinant wild-type and mutant G196V from E. coli
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recombinant wild-type, chimeric mutants, C-terminal deletion mutants, and D113 mutants from E. coli
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recovered using dye-affinity chromatography in the presence of salt
Talon metal (Co2+) affinity resin column chromatography
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partial
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recombinant chimeric protein from E. coli
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recombinant chimeric protein from E. coli
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recombinant enzyme from E. coli
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recombinant enzyme from E. coli