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2.3.3.16: citrate synthase (unknown stereospecificity)

This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.

Word Map on EC 2.3.3.16

Reaction

acetyl-CoA
+
H2O
+
oxaloacetate
=
citrate
+
CoA

Synonyms

bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.16 citrate synthase (unknown stereospecificity)

Purification

Purification on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms
-
antibody studies
-
both isoenzymes
-
commercial preparation
heat step and dye-ligand affinity chromatography
HisTrap column chromatography and Source 15Q column chromatography
Matrex Red Gel A affinity column chromatography
mitochondrial enzyme, antibody studies
-
mitochondrial isozyme
-
mitochondrial isozyme partially, glyoxysomal isozyme to homogeneity
-
native and recombinant wild-type, the latter from E. coli and recombinant S43C mutant enzyme from E. coli
-
Ni-NTA column chromatography
Ni-NTA column chromatography, Superdex 200 gel filtration, and Sephadex G25 gel filtration
Ni2+-loaded iminodiacetic acid Sepharose column chromatography and Sephadex 200 gel filtration
-
nickel-affinity column chromatography
partial
recombinant chimeric mutants from E. coli
-
recombinant chimeric protein from E. coli
recombinant enzyme from E. coli
recombinant from E. coli
-
recombinant His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase from E. coli
-
recombinant peroxisomal isozyme CS II from E. coli
-
recombinant protein
recombinant protein expressed from plasmid
-
recombinant wild-type and mutant G196V from E. coli
-
recombinant wild-type, chimeric mutants, C-terminal deletion mutants, and D113 mutants from E. coli
-
recombinantz enzyme
-
recovered using dye-affinity chromatography in the presence of salt
Talon metal (Co2+) affinity resin column chromatography