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2.3.3.16: citrate synthase (unknown stereospecificity)

This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.

Word Map on EC 2.3.3.16

Reaction

acetyl-CoA
+
H2O
+
oxaloacetate
=
citrate
+
CoA

Synonyms

bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.16 citrate synthase (unknown stereospecificity)

Specific Activity

Specific Activity on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00023 - 0.00028
-
wild-type
0.00078
recombinant in transgenic Arabidopsis thaliana plants
0.008
-
recombinant purified mutant G196V
0.02
-
recombinant enzyme in crude E. coli enzyme extract
0.045
-
crude extract
0.083
-
recombinant purified wild-type
0.23 - 0.28
-
-
0.76
-
isoenzyme CSI, Matrex Gel Red-A
0.764
-
purified glyoxysomal isozyme
0.78
transgenic Arabidopsis thaliana plants overexpressing the Daucus carota citrate synthase
0.87
-
partially purified enzyme
0.99
-
glucose-grown cells, pH and temperature not specified in the publication
1.25
-
partially purified enzyme
1.26
-
acetate-grown cells, pH and temperature not specified in the publication
1.32
-
isoenzymes CSI and CSII, DEAE-Sephacel
102.7
-
purified enzyme
11.5
-
isoenzyme CSII, Matrex Gel Red-A
112.5
1190
-
partially purified enzyme
120
-
purified recombinant chimeric E. coli-type protein with small Acinetobacter domain
14
-
partially purified enzyme
18
-
purified enzyme
18.8
-
partially purified enzyme
2.6
-
partially purified enzyme
213
-
purified fusion protein of citrate synthase and mitochondrial malate dehydrogenase, coupled assay
230
-
purified enzyme
24
-
purified enzyme
25.8
-
purified enzyme
28
-
purified dimeric and hexameric enzyme form
288
-
purified enzyme
30
-
purified recombinant enzyme from E. coli
35.1
-
purified enzyme
4.7
-
isozymes CS I and CS II
4.71
-
isoenzyme CSI, Sephadex G-200
40
37°C, pH 8.0
41.5
citrate synthase activity of a purified methylcitrate synthase
45
-
purified wild-type enzyme
471.7
-
partially purified enzyme
50 - 60
-
partially purified enzyme
51.4
-
wild-type
52.3
-
purified enzyme
57
-
purified recombinant chimeric Acinetobacter-type protein with small E. coli domain
60
-
dimeric and hexameric enzyme
62.6
-
purified enzyme
64
-
purified enzyme
664.2
-
-
70
-
purified recombinant isozyme CS II
79
purified enzyme
88
-
purified wild-type enzyme
additional information