2.3.3.8: ATP citrate synthase

This is an abbreviated version!
For detailed information about ATP citrate synthase, go to the full flat file.

Reaction

Synonyms

ACL, ACL1, ACL2, ACLA, ACLB, ACLY, adenosine triphosphate citrate lyase, ATP citrate (pro-S)-lyase, ATP citrate lyase, ATP citrate lyase isoform 2, ATP-citrate lyase, ATP-citrate synthase A-2, ATP-citrate synthase alpha chain protein 2, ATP-citric lyase, ATP:citrate lyase, ATP:citrate oxaloacetate lyase ((pro-3S)-CH2COO--> acetyl-CoA) (ATP-dephosphorylating), ATP:citrate oxaloacetate-lyase (pro-3S-CH2COO->acetyl-CoA, ATP dephosphorylating), ATP:citrate oxaloacetate-lyase CoA-acetylating and ATP-dephosphorylating, Citrate cleavage enzyme, citrate-ATP lyase, citric cleavage enzyme, Earlier Degraded Tapetum1, EC 4.1.3.8, EDT1, Os11g0696200

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.3 Acyl groups converted into alkyl groups on transfer

                2.3.3.8 ATP citrate synthase

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Results	in table
16	Activating Compound
7726	AA Sequence
12	Application
1	CAS Registry Number
23	Cloned(Commentary)
7	Crystallization (Commentary)
446	Disease/ Diagnostics
20	Expression
53	General Information
6	General Stability
18	IC50 Value
123	Inhibitors
3	kcat/KM [mM/s]
7	Ki Value [mM]
57	KM Value [mM]
49	Localization
20	Metals/Ions
50	Molecular Weight [Da]
56	Natural Substrates/ Products (Substrates)
174	Organism
2	Oxidation Stability
9	Pathway
98	PDB ID
11	pH Optimum
4	pH Range
2	pH Stability
1	pI Value
26	Posttranslational Modification
9	Protein Variants
38	Purification (Commentary)
8	Reaction
1	Reaction Type
155	Reference
132	Source Tissue
20	Specific Activity [micromol/min/mg]
6	Storage Stability
153	Substrates and Products (Substrate)
37	Subunits
132	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]

Engineering

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Engineering on EC 2.3.3.8 - ATP citrate synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H305A

2 entries

H274A

Chlorobium limicola

-

mutation abolishes both the catalytic activity and phosphorylation of the enzyme by ATP

488225

D1026N

Homo sapiens

P53396

mutant enzyme has 2% activity compared to wild-type enzyme

757803

H760A

Homo sapiens

-

the turnover number of the mutant is similar to that of wild type in the absence of citrate and CoA, yet significantly less than that of wild type enzyme in the presence of both citrate and CoA

718938

H975A

Homo sapiens

P53396

mutant enzyme has 25% activity compared to wild-type enzyme

757803

additional information

3 entries

H305A

Aspergillus niger

-

inactive mutant of isoform Acl1

736591

H305A

Aspergillus niger ATCC 1015

-

inactive mutant of isoform Acl1

-

additional information

Arabidopsis thaliana

-

decreased enzyme activity by use of antisense RNA, even plants with moderately reduced enzyme activity have a complex, bonsai phenotype with miniaturized organs, smaller cells, aberrant plastid morphology, reduced cuticular wax deposition and hyperaccumulation of starch, anthocyanin, and stress-related mRNAs in vegetative tissue. Exogenous malonate complements phenotype

660133

additional information

Chlorobaculum tepidum

-

expression of holo-ATP citrate synthase as well as its two individual subunits in Escherichia coli. Purified recombinant subunits are able to reconstitute the holo-enzyme in vitro, with activity levels similar to that of recombinant holo-enzyme prepared from coexpressed genes. Residues of each subunit contribute to different aspects of the catalytic mechanism, so both proteins contribute to the active site

674301

additional information

Mus musculus

-

enzyme knockout mutant leads to a significant impairment of glucose-dependent lipid synthesis and an elevation of mitochondrial membrane potential. Cells lacking ATP citrate synthase display decreased cytokine-stimulated cell proliferation. In contrast, these cells resist cell death induced by either cytokine or glucose withdrawal. The mutation significantly impairs Akt-mediated tumorigenesis in vivo

676255