Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.3.9: malate synthase

This is an abbreviated version!
For detailed information about malate synthase, go to the full flat file.

Word Map on EC 2.3.3.9

Reaction

acetyl-CoA
+
glyoxylate
 +
H2O
=
(S)-malate
 +
CoA

Synonyms

EC 4.1.3.2, GCE, GCE(13-573), glcB, glyoxylate cycle enzyme, glyoxylate transacetase, glyoxylic transacetase, L-malate glyoxylate-lyase (CoA-acetylating), malate synthase, malate synthase 1, malate synthase A, malate synthase G, malate synthase H, malate synthetase, malic synthetase, malic-condensing enzyme, MLS, MSA, MSG, MSH, SSO1334

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.9 malate synthase

Engineering

Engineering on EC 2.3.3.9 - malate synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C438S
-
residue in the acetyl-CoA binding pocket
C617S
D631N
R338K
D475N
-
the glyoxylate cycle enzyme mutant completely loses malate synthase activity but retains isocitrate lyase activity
R187K
-
the glyoxylate cycle enzyme mutant completely loses malate synthase activity but retains isocitrate lyase activity
C619S
D453A
0.06% of wild-type activity
P186R
-
more thermostable and thermoactive than wild-type
R171L
0.2% of wild-type activity, reduced expression at room temperature, expression of soluble protein at 15°C
T8P/L9P
-
more thermostable than wild-type
T8P/L9P/P186R
-
more thermostable and thermoactive than wild-type
D453A
-
0.06% of wild-type activity
-
R171L
-
0.2% of wild-type activity, reduced expression at room temperature, expression of soluble protein at 15°C
-
additional information