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2.3.3.9: malate synthase

This is an abbreviated version!
For detailed information about malate synthase, go to the full flat file.

Word Map on EC 2.3.3.9

Reaction

acetyl-CoA
+
glyoxylate
+
H2O
=
(S)-malate
+
CoA

Synonyms

EC 4.1.3.2, GCE, GCE(13-573), glcB, glyoxylate cycle enzyme, glyoxylate transacetase, glyoxylic transacetase, L-malate glyoxylate-lyase (CoA-acetylating), malate synthase, malate synthase 1, malate synthase A, malate synthase G, malate synthase H, malate synthetase, malic synthetase, malic-condensing enzyme, MLS, MSA, MSG, MSH, SSO1334

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.9 malate synthase

Molecular Weight

Molecular Weight on EC 2.3.3.9 - malate synthase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
120000
Cucurbita sp.
-
sucrose density gradient centrifugation
160000
170000
-
gel filtration, ultracentrifugation, sucrose density gradient centrifugation
175000
-
2 * 175000, SDS-PAGE
180000
-
high speed equilibrium sedimentation
186000
-
small-angle X-ray scattering technique
200000
-
gel filtration
22000
-
8 * 22000, SDS-PAGE
250000
350000
-
gel filtation
420000
gel filtration
480000
Cucurbita sp.
-
sucrose density gradient centrifugation
510000
-
gel filtration
52000 - 54000
-
gel filtration, equilibrum sedimentation centrifugation, light scattering, two distinct forms of enzyme
520000
55000
-
gel filtration
56000
575000
-
sucrose density gradient centrifugation
58000
60000
60900
x * 60900, calculated
61000
-
4 * 61000, SDS-PAGE
61360
-
2 * 61360, calculation from nucleotide sequence
62000
63000
630000
64000
-
x * 64000, SDS-PAGE
65000
66000
-
3 * 66000, SDS-PAGE
67000
-
3 * 67000, SDS-PAGE
70000
730000
-
gel filtration
750000
-
sucrose density gradient centrifugation
763000
-
2 * 763000, SDS-PAGE
79400
-
x * 79400, predicted and observed (SDS-PAGE)
80000
82000
86000
x * 86000, GST-enzyme fusion protein, SDS-PAGE
90000
1 * 90000, SDS-PAGE
additional information
-
by incubation with 5 mM ATP the high molecular weight enzyme form MSH is converted to the low molecular weight enzyme form MSL