2.3.3.9: malate synthase
This is an abbreviated version!
For detailed information about malate synthase, go to the full flat file.
Word Map on EC 2.3.3.9
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2.3.3.9
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isocitrate
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peroxisomal
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icl
-
glyoxysomal
-
catalase
-
citrate
-
tricarboxylic
-
tuberculosis
-
shunt
-
cotyledons
-
seedling
-
microbodies
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cucumber
-
gluconeogenesis
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beta-oxidation
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4.1.3.1
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anaplerotic
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fumarase
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thiolase
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cucumis
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phosphotransacetylase
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trosy
-
hydroxypyruvate
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acetate-grown
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agriculture
-
3-ketoacyl-coa
-
pumpkin
- 2.3.3.9
- isocitrate
- peroxisomal
- icl
- glyoxysomal
- catalase
- citrate
-
tricarboxylic
- tuberculosis
-
shunt
- cotyledons
- seedling
- microbodies
- cucumber
-
gluconeogenesis
-
beta-oxidation
-
4.1.3.1
-
anaplerotic
- fumarase
-
thiolase
-
cucumis
- phosphotransacetylase
-
trosy
- hydroxypyruvate
-
acetate-grown
- agriculture
- 3-ketoacyl-coa
-
pumpkin
Reaction
Synonyms
EC 4.1.3.2, GCE, GCE(13-573), glcB, glyoxylate cycle enzyme, glyoxylate transacetase, glyoxylic transacetase, L-malate glyoxylate-lyase (CoA-acetylating), malate synthase, malate synthase 1, malate synthase A, malate synthase G, malate synthase H, malate synthetase, malic synthetase, malic-condensing enzyme, MLS, MSA, MSG, MSH, SSO1334
ECTree
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Temperature Stability
Temperature Stability on EC 2.3.3.9 - malate synthase
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40
45
50
55
60
additional information
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heating for 3 min, complete inactivation of enzyme form MSL and 65% loss of activity of enzyme form MSH, slight increase of heat stability in presence of glyoxylate and/or Mg2+
45
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50 mM Tris-HCl, pH 8.0, without KCl or with 0.2 M KCl, half-life: 25 min
45
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pH 7.6, 5 min, about 60% loss of activity of enzyme form MSL, enzyme form MSH is stable up to
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50 mM Tris-HCL, pH 8.0, without KCl or with 0.2 M KCl, half-life: 3 min
55
20 min, 80% residual activity, in presence of 0.2 M guanidine HCl, 10 min, 50% residual activity
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25 mM Tris-HCl, pH 7.0, without KCl, half-life: 168 min, 25 mM Tris-HCL, pH 7.0, with KCl, half-life: 30 min, 25 mM glycine-NaOH buffer, pH 8.5, without KCl, half-life: 750 min, 25 mM glycine-NaOH buffer, pH 8.5, with 0.2 KCl, half-life: 50 min, 25 mM glycine-NaOH buffer, pH 9.0, without KCl, half-life: 522 min, 25 mM glycine-NaOH buffer, pH 9.0, with 0.2 KCl, half-life: 12 min, 25 mM Tris-HCL, pH 6.86, with 0.2 M KCl, half-life: 2 min