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2.4.1.1: glycogen phosphorylase

This is an abbreviated version!
For detailed information about glycogen phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.1

Reaction

[(1->4)-alpha-D-glucosyl]n
+
phosphate
=
[(1->4)-alpha-D-glucosyl]n-1
 +
alpha-D-glucose 1-phosphate

Synonyms

1,4-alpha-glucan phosphorylase, alpha-1,4 glucan phosphorylase, alpha-1,4-glycan phosphorylase, alpha-glucan phosphorylase, alpha-glucan phosphorylase H, alpha-glucan/maltodextrin phosphorylase, alphaGP, amylopectin phosphorylase, amylophosphorylase, CcStP, cyosolic phosphorylase, GlgP, glucan phosphorylase, glucosan phosphorylase, glycogen phosphorylase, glycogen phosphorylase a, glycogen phosphorylase b, glycogen phosphorylase-a, GP, GP b, GPA, GPase, GPase a, GPb, GPBB, GPH, granulose phosphorylase, MalP, maltodextrin phosphorylase, More, muscle glycogen phosphorylase, muscle phosphorylase, muscle phosphorylase a and b, myophosphorylase, PF1535, Phb, PHO, Pho 2, Pho1, phosphorylase a, phosphorylase b, phosphorylase, alpha-glucan, plastidial phosphorylase, polyphosphorylase, potato phosphorylase, RMGPa, rmGPb, SP, starch phosphorylase, starch phosphorylase H, stGP, StP, tGPGG, type L alpha-glucan phosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.1 glycogen phosphorylase

Storage Stability

Storage Stability on EC 2.4.1.1 - glycogen phosphorylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 200 mM NaF, 1 month, no loss of activity
-
-18°C, phosphorylase A or C, at least 1 month
-
-20°C, 50% glycerol, at least 4 months
-
-20°C, cytoplasmic isozyme, 12 months, approx. 30% loss of activity
-
-20°C, partially purified, lyophilized, at least 2 months
-
-20°C, phosphorylase B, at least 1 month
-
-20°C, purified, lyophilized, 30% loss of activity within 3 months
-
0-4°C, at least 1 month
-
0°C, at least 3 months
-
0°C, heart isozyme Ia, stable in the presence of 2-mercaptoethanol
-
0°C, several days
-
3°C, 2 weeks, no loss of activity
-
4°C, 2 weeks, 10% loss of activity
-
4°C, 20-25 mg/ml in 100 mM succinate buffer, 0.02% NaN3, 2-3 months, little loss of activity
-
4°C, 3.5 M (NH4)2SO4 in solution of neutral pH-value, several months, no loss of activity
-
4°C, 30 days, 20% loss of activity
-
6°C, 3 months
-
7°C, isozyme b, inactivation within 48 h, isozyme a: at least 48 h, in crude extract
-
bovine serum albumin prevents surface inactivation during storage
-
glycerol stabilizes purified enzyme during storage
purified enzyme in 50 mM imidazole, pH 7.0, 1 mM EDTA, 1 mM DTT, at 4°C, at least 2 weeks stable
-
storage in frozen or lyophilized state, crystalline human, not rabbit, phosphorylase a or b, stable to
-