2.4.1.101: alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
This is an abbreviated version!
For detailed information about alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, go to the full flat file.
Word Map on EC 2.4.1.101
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2.4.1.101
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n-glycans
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n-linked
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glycosyltransferases
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mannosidase
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oligomannose
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high-mannose
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complex-type
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paucimannose
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swainsonine
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golgi-resident
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beta-1,4-galactosyltransferase
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paucimannosidic
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man3glcnac2
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medial-golgi
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glcnacman5glcnac2
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biotechnology
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medicine
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molecular biology
- 2.4.1.101
- n-glycans
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n-linked
- glycosyltransferases
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mannosidase
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oligomannose
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high-mannose
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complex-type
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paucimannose
- swainsonine
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golgi-resident
- beta-1,4-galactosyltransferase
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paucimannosidic
- man3glcnac2
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medial-golgi
- glcnacman5glcnac2
- biotechnology
- medicine
- molecular biology
Reaction
Synonyms
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-, alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, alpha3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, AtGnTI, beta-1-2 GlcNAc transferase, ChGnTI, EC 2.4.1.51, GlcNAc transferase I, GlcNAc-T I, GlcNAc-TI, GlcNAcT-I, gly-12, GLY-13, gly-14, GnT I, GnT-I, GnT1, GnTI, Golgi N-glycan branching N-acetylglucosaminyltransferase I, HuGnTI, MGAT1, N-acetylglucosaminyltransferase I, N-acetylglucosaminyltransferases I, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, NT, pigGnT-I, PtGnTI, TbGnTI, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, UDP-GlcNac:alpha3-D-mannoside beta1,2-Nacetylglucosaminyltransferase I, UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferase I, UDP-N-acetyl-D-glucosamine:glycoprotein (N-acetyl-D-glucosamine to alpha-D-mannosyl-1,3-(R1)-beta-D-mannosyl-R2) beta-1,2-N-acetyl-D-glucosaminyltransferase, UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase
ECTree
2.4.1.101 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdvanced search results
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results (Engineering
Engineering on EC 2.4.1.101 - alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D144N
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D144N muation in the GnTI of cgl mutant lacking GnTI activity is molecular basis of the defect. The mutation creates an additional N-glycosylation site, which interferes with the proper folding of the enzyme
C907T
point mutation in mutant cell line Lec1A.3E and Lec1A.5J, altering interactions important for stabilization of the structure, correction of the mutation by site-directed mutagenesis restores enzyme activity
G634A
point mutation in mutant cell line Lec1A.2C, disrution of DxD motif responsible for interaction with UDP-GlcNAc and Mn2+, correction of the mutation by site-directed mutagenesis restores enzyme activity
C121A
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active mutant enzyme. After 1 h, the wild-type MBP-hGnT1(D103) and C121A mutant convert more than 50% of Man5-RNAse B to RNAse B-GlcNAc2-Man5-GlcNAc
C121D
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mutant enzyme shows no transfer of GlcNAc from UDP-GlcNAc to the glycoprotein acceptor Man5-RNAse B
C121S
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active mutant enzyme. After 1 h, the C121S mutant shows less than 25% conversion of Man5-RNAse B to RNAse B-GlcNAc2-Man5-GlcNAc
C121T
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mutant enzyme shows no transfer of GlcNAc from UDP-GlcNAc to the glycoprotein acceptor Man5-RNAse B
D77N
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exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity
R120A/C121H
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active mutant enzyme. The R120A/C121H mutant is less active than the wild type and the single mutants. It converts more than half of the Man5-RNAse B to RNAse B-GlcNAc2-Man5-GlcNAc
R83S
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exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity
R85S
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exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity
D144N
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expression of D144N muatant enzyme of rabbit in cgl mutant of Arabidopsis thaliana lacking GnTI activity (caused by a D144N mutation in GnTI) can partially restore complex N-glycan formation
more.
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deleting seven C-terminal amino acids of the enzyme reduces enzymatic activity by 40%
G320D
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mutant enzyme does not show any activity in an HPLC analysis. In the case of pig cell transfectants with pigGnT-I(320), cell surface carbohydrate structures are significantly altered and its antigenicity to human serum is reduced
T223A
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isozyme B, improvement of properties of isozyme B to the level of isozyme A
additional information
additional information
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natural point mutation contains mRNA but no enzyme activity
additional information
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AAA, ER steady-state location with little Golgi labelling
additional information
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Elisa experiments show that cgl1-1 mutant of Arabidopsis lacking N-acetylglucosaminyltransferase I activity, is fully complemented by YFP-labeled Arabidopsis enzyme (similar level of proteins with complex glycans), but only partially complemented by human enzyme (lower levels of proteins with complex glycans), chimeric enzyme with similar levels compared to wild-type, sub-cellular localization of the human enzyme is partly distinct, the human enzyme is easily cleaved of its transmembrane anchor
additional information
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RAA, localisation in ER and Golgi membrane, more pronounced labeling in the ER
additional information
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site-directed mutagenesis of single arginine residues within cytoplasmic tail are sufficient to promote rapid Golgi targeting of Golgi-resident N-acetylglucosaminyltransferase I, an intact ER export motif is essential for proper in vivo function, transport is COPII-dependent, exchange of the cytoplasmic tail with the corresponding part of other plant glycosylation enzymes does not alter the Golgi targeting, complementation experiments with cg/1 Arabidopsis thaliana lacking an active N-acetylglucosaminyltransferase I
additional information
eukaryotic N-glycosylation pathways are dependent of N-acetylglucosaminyltransferase I (GnTI), a key glycosyltransferase opening the door to the formation of complex-type N-glycans by transferring a N-acetylglucosamine residue onto the Man5GlcNAc2 intermediate. In contrast, glycans N-linked to Chlamydomonas reinhardtii proteins arise from a GnTI-independent Golgi processing of oligomannosides giving rise to Man5GlcNAc2 substituted eventually with one or two xylose(s). Chlamydomonas reinhardtii proteins carry linear Man5GlcNAc2 instead of the branched structure usually found in eukaryotes. Moreover, characterization of the lipid-linked oligosaccharide precursor demonstrated that Chlamydomonas reinhardtii exhibit a Glc3Man5GlcNAc2 dolichol diphosphate precursor. This precursor is then trimmed into a linear Man5GlcNAc2 that is not substrate for GnTI. Expression of GnTI cDNAs originated from Arabidopsis and the diatom Phaeodactylum tricornutum cause no modification of the N-glycans in the GnTI transformed cells. Cells expressing GnTI exhibit an altered phenotype with large vacuoles, increase of ROS production and accumulation of starch granules, suggesting the activation of stress responses likely due to the perturbation of the Golgi apparatus
additional information
complete abolition of GlcNAc-TI activity requires the generation of a triple knock-out strain, knockoing out the three genes encoding the enzyme, i.e. gly-12, gly-13 and gly-14. The multiple hexose residues of the N-glycans of the gly-12/gly-13/gly-14 triple mutant are not just mannose, but include galactoses in three different positions (beta-intersecting, beta-bisecting and alpha-terminal) on isomeric forms of Hex4-8HexNAc2 structures, some of these structures are fucosylated and/or methylated, determination of the N-glycome of 12/gly-13/gly-14 triple GlcNAc-TI knock-out strain
additional information
complete abolition of GlcNAc-TI activity requires the generation of a triple knock-out strain, knockoing out the three genes encoding the enzyme, i.e. gly-12, gly-13 and gly-14. The multiple hexose residues of the N-glycans of the gly-12/gly-13/gly-14 triple mutant are not just mannose, but include galactoses in three different positions (beta-intersecting, beta-bisecting and alpha-terminal) on isomeric forms of Hex4-8HexNAc2 structures, some of these structures are fucosylated and/or methylated, determination of the N-glycome of 12/gly-13/gly-14 triple GlcNAc-TI knock-out strain
additional information
complete abolition of GlcNAc-TI activity requires the generation of a triple knock-out strain, knockoing out the three genes encoding the enzyme, i.e. gly-12, gly-13 and gly-14. The multiple hexose residues of the N-glycans of the gly-12/gly-13/gly-14 triple mutant are not just mannose, but include galactoses in three different positions (beta-intersecting, beta-bisecting and alpha-terminal) on isomeric forms of Hex4-8HexNAc2 structures, some of these structures are fucosylated and/or methylated, determination of the N-glycome of 12/gly-13/gly-14 triple GlcNAc-TI knock-out strain
additional information
-
complete abolition of GlcNAc-TI activity requires the generation of a triple knock-out strain, knockoing out the three genes encoding the enzyme, i.e. gly-12, gly-13 and gly-14. The multiple hexose residues of the N-glycans of the gly-12/gly-13/gly-14 triple mutant are not just mannose, but include galactoses in three different positions (beta-intersecting, beta-bisecting and alpha-terminal) on isomeric forms of Hex4-8HexNAc2 structures, some of these structures are fucosylated and/or methylated, determination of the N-glycome of 12/gly-13/gly-14 triple GlcNAc-TI knock-out strain
additional information
1 difference to published sequence with accession number U65791: C1340 is A1340
additional information
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construction of chimeric proteins with different portions of the N-terminal ectodomain of the enzyme, modification to ectodomain of type II surface membrane protein, chimeras are retained in the Golgi apparatus
additional information
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a truncated, soluble enzyme form, consisting of stem region and catalytic domain, is accumulated in the Golgi apparatus prior to secretion
additional information
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Elisa experiments show that cgl1-1 mutant of Arabidopsis lacking N-acetylglucosaminyltransferase I activity, is fully complemented by YFP-labeled Arabidopsis enzyme (similar level of proteins with complex glycans), but only partially complemented by human enzyme (lower levels of proteins with complex glycans), chimeric enzyme with similar levels compared to wild-type, sub-cellular localization of the human enzyme is partly distinct, the human enzyme is easily cleaved of its transmembrane anchor
additional information
GlcNAc and tet-induced Mgat1 increases the levels of many metabolites in HeLa cells, and HEK293 cells, tet-induced Mgat1 changes in the N-glycan distributions, , N-glycans profiles of transgenic HeLa and HEK-293 cells, overview
additional information
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GlcNAc and tet-induced Mgat1 increases the levels of many metabolites in HeLa cells, and HEK293 cells, tet-induced Mgat1 changes in the N-glycan distributions, , N-glycans profiles of transgenic HeLa and HEK-293 cells, overview
additional information
MGAT1 protein and mRNA levels are markedly reduced in cells infected with lentiviral expressing MGAT1 shRNA-1 or MGAT1-shRNA-2. Knockdown of MGAT1 leads to an increase in the G1-phase cells with a decrease in S-phase cells and G2/M-phase cells. Downregulation of MGAT1 significantly inhibits migration of GBM cells
additional information
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construction of a series of chimeric N-acetylglucosaminyltransferase I (GnTI) mutants by to investigate the contribution of the cytoplasmic, transmembrane, and stem region the enzyme for its cis/medial-Golgi localization and for protein-protein interaction in the Golgi apparatus. The individual GnTI protein domains, i.e. cytoplasmic tail, transmembrane domain and luminal stem region, are replaced with those from the well-known trans-Golgi enzyme alpha2,6-sialyltransferase and transiently expressed in Nicotiana benthamiana. A chimeric GnTI variant with altered sub-Golgi localization is not able to complement the GnTI-dependent glycosylation defect. Generation of transgenic Arabdiopsis thaliana gntI plants expressing the chimeric CTS domai region fused to the catalytic domain of Arabidopsis thaliana GnTI enzyme and expression in enzyme activity-deficient Nicotiana benthamiana plants under control of the endogenous GnTI promoter. The Arabidopsis enzyme chimeric constructs can complement the inactive the N-glycan processing defect of tobacco mutant plants
additional information
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transient transfection of isolated protoplasts of Arabidopsis thaliana defective mutant with cDNA in sense and antisense orientation
additional information
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construction of antisense transgenic plants via Agrobacterium tumefaciens transfection of the own gene in antisense orientation, regulation analysis
additional information
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the fusion of the human beta 1,4-galactosyltransferase with the targeting domain of Nicotiana N-acetylglucosaminyltransferase I does not increase the level of beta 1,4-galactosylation in alfalfa
additional information
eukaryotic N-glycosylation pathways are dependent of N-acetylglucosaminyltransferase I (GnTI), a key glycosyltransferase opening the door to the formation of complex-type N-glycans by transferring a N-acetylglucosamine residue onto the Man5GlcNAc2 intermediate. In contrast, glycans N-linked to Chlamydomonas reinhardtii proteins arise from a GnTI-independent Golgi processing of oligomannosides giving rise to Man5GlcNAc2 substituted eventually with one or two xylose(s). Chlamydomonas reinhardtii proteins carry linear Man5GlcNAc2 instead of the branched structure usually found in eukaryotes. Moreover, characterization of the lipid-linked oligosaccharide precursor demonstrated that Chlamydomonas reinhardtii exhibit a Glc3Man5GlcNAc2 dolichol diphosphate precursor. This precursor is then trimmed into a linear Man5GlcNAc2 that is not substrate for GnTI. Expression of GnTI cDNAs originated from Arabidopsis and the diatom Phaeodactylum tricornutum cause no modification of the N-glycans in the GnTI transformed cells. Cells expressing GnTI exhibit an altered phenotype with large vacuoles, increase of ROS production and accumulation of starch granules, suggesting the activation of stress responses likely due to the perturbation of the Golgi apparatus
additional information
-
eukaryotic N-glycosylation pathways are dependent of N-acetylglucosaminyltransferase I (GnTI), a key glycosyltransferase opening the door to the formation of complex-type N-glycans by transferring a N-acetylglucosamine residue onto the Man5GlcNAc2 intermediate. In contrast, glycans N-linked to Chlamydomonas reinhardtii proteins arise from a GnTI-independent Golgi processing of oligomannosides giving rise to Man5GlcNAc2 substituted eventually with one or two xylose(s). Chlamydomonas reinhardtii proteins carry linear Man5GlcNAc2 instead of the branched structure usually found in eukaryotes. Moreover, characterization of the lipid-linked oligosaccharide precursor demonstrated that Chlamydomonas reinhardtii exhibit a Glc3Man5GlcNAc2 dolichol diphosphate precursor. This precursor is then trimmed into a linear Man5GlcNAc2 that is not substrate for GnTI. Expression of GnTI cDNAs originated from Arabidopsis and the diatom Phaeodactylum tricornutum cause no modification of the N-glycans in the GnTI transformed cells. Cells expressing GnTI exhibit an altered phenotype with large vacuoles, increase of ROS production and accumulation of starch granules, suggesting the activation of stress responses likely due to the perturbation of the Golgi apparatus
additional information
transient transfection of isolated protoplasts of Arabidopsis thaliana defective mutant with cDNA in sense and antisense orientation
additional information
-
transient transfection of isolated protoplasts of Arabidopsis thaliana defective mutant with cDNA in sense and antisense orientation
additional information
construction of antisense transgenic plants via Agrobacterium tumefaciens transfection of the own gene in antisense orientation, regulation analysis
additional information
-
construction of antisense transgenic plants via Agrobacterium tumefaciens transfection of the own gene in antisense orientation, regulation analysis
