Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.102: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.102

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl/threonyl-[protein]
=
UDP
 +
O3-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Synonyms

2beta-1,6-acetylglucosaminyltransferase, acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta-(1-6)-, acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta-(1-6)-, A, bC2GnT-M, beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, beta-1,6-N-acetylglucosaminyltransferase, beta1,6-N-acetylglucosaminyltransferase, beta6-N-acetylglucosaminyltransferase, Bo17, C2 beta6GnT1, C2/4GnT, C24GNT, C2GlcNAcT-I, C2GlcNAcT-II, C2GlcNAcT-III, C2GnT, C2GnT-1, C2GnT-I, C2GnT-L, c2GnT-M, C2GnT1, C2GnT2, C2GnT3, C2GNTM, C4GnT, core 2 acetylglucosaminyltransferase, core 2 beta-1, 6-N-acetylglucosaminyltransferase-1, core 2 beta-1,6 N-acetylglucosaminyltransferase, core 2 beta-1,6-N-acetylglucosaminyltransferase, core 2 beta1,6 N-acetylglucosaminyltransferase, core 2 beta1,6 N-acetylglucosaminyltransferase-I, core 2 beta1,6-N-acetyl-glycosaminyltransferase, core 2 beta1,6-N-acetylglucosaminyl transferase 1, core 2 beta1,6-N-acetylglucosaminyltransferase, core 2 beta1,6-N-acetylglucosaminyltransferase I, core 2 beta1,6-N-acetylglucosaminyltransferase-I, core 2 beta6-GlcNAc-transferase, core 2 beta6-N-acetylglucosaminyltransferase, core 2 GlcNAc-T, core 2 N-acetylglucosaminyltransferase, Core 2 N-acetylglucosaminyltransferase 2/M, core 2 N-acetylglucosaminyltransferase M, core 2 N-acetylglucosaminyltransferase-1, core 2 N-acetylglucosaminyltransferase-M, core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type, core 2beta 1,6 N-acetylglucosaminyltransferase, core 2beta-1,6-acetylglucosaminyltransferase, core 6-beta-GlcNAc-T, core 6-beta-GlcNAc-transferase A, core2 1-6-N-glucosaminyltransferase-I, core2 beta(1,6)-N-acetyglucosaminyltransferase-I, core2 beta-1,6-N-acetylglucosaminyltransferase, core2 beta-1,6-N-acetylglucosaminyltransferase-1, Core2beta1,6GnT type M, core2GnT, GCNT1, gcnt3, gcnt4, GnT-III, GNTM, More, mucin-type core 2 1,6-N-acetylglucosaminyltransferase enzyme, mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase, O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-mucin beta-(1-6)-acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta-(1-6)-acetylglucosaminyltransferase A

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.102 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Cloned

Cloned on EC 2.4.1.102 - beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CHO cells stably transfected with a pSEcTag plasmid, This plasmid contains bC2GnT-M cDNA devoid of the 5'-sequence coding the cytoplasmic tail and transmembrane domain
-
cloning and expression of isozyme 3 in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, secretion of the enzyme
coexpression of isozyme 3 with leukosialin as substrate in CHO cells for in vivo activity assay of the enzyme
cotransfection and expression of Epstein-Barr virus (EBV) miRNA miR-BART1-5p and GCNT3 in HEK-293T and SGC7901 cells
DNA sequence determination and analysis
expression in human pancreatic cancer cell line Panc1-MUC1, which lacks enzyme C2GnT activity, alters the expression of MUC1-epitope in the same cell line
-
expression in PC12 cells
-
expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, truncated enzyme form
-
expression of the soluble catalytic domain in insect cells
-
functional expression in CHO cells, direction of enzyme activity onto the cell surface, a soluble chimeric enzyme form shows also core 4 6beta-GalNAc transferase activity, DNA and amino acid sequence determination
gene Bo17, DNA and amino acid sequence determination and analysis, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. The first one is enzymatically active, the second is inactive and results from the alternative splicing of the region encoding the catalytic site of the enzyme, enzyme activities in infected cells, overview
gene GCNT1 , recombinant expression in CHO-K1 cells. Glycosylation-related genes in CHO-K1 cells are present, but strictly suppressed and regulated. CHO cells are transiently co-transfected with plasmids encoding extended C1 beta3GnT3, C2 beta6GnT1, or C3 beta3GnT6 with or without ST6Gal I or CHST4, respectively, and resulting O-glycan and core chain spectrum, overview
gene Gcnt1, identification of the Gcnt1 promoter, identification of a putative distal regulatory element of the Gcnt1 gene by mapping H3K4me2 and H3K27me3 across the Gcnt1 locus, sequence comparisons, phylogenetic analysis of promoters, overview. The distal regulatory region is an enhancer forGcnt1 for which STAT4 is functionally important. Prolonged T-bet binding to the Gcnt1 enhancer
gene GCNT2, recombinant expression in COS7 cells
-
gene GCNT3 expression analysis in pancreatic cancer and in healthy pancreatic tissue, gene expression profiling and transcriptome analysis
gene GCNT3, quantitative reverse-transcription PCR enzyme expression analysis
gene GCNT3, recombinant expression of C-terminally 3xMyc-6His-tagged wild-type enzyme and deletion mutant mutGCNT3
gene GCNT3, recombinant overexpression in SW620 and SW5FU cell lines, genomic analysis of GCNT3 overexpression, quantitative RT-PCR enzyme expression analysis, genomic and proteomic landscapes of GCNT3 are linked to cell cycle and response to drug pathways. GCNT3 overexpression does not significantly change cell growth in Caov3 cells, VEGFA expression is downregulated in GCNT3 Caov3 cells
HH and BW5147 cells are transfected with either wild-type or mutant human C2GnT-I cDNA. Expression of C2GnT-I makes HH cells susceptible to galectin-1
-
mutant enzymes are expressed in HEK-293T cells
overexpression in CHO cells as protein A-tagged fusion protein
-
overexpression in KM3 leukemia cell line
-
recombinant expression of C-terminally c-Myc-tagged betaC2GnT-M in Panc1 cells, transient expression of GFP-tagged C2GnT-M in HeLa cells. Yeast two-hybrid analysis of the interactions between KRT1 protein head, tail, or rod domain and the cytoplasmic peptides of hC2GnT-M
the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. As opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. Bo17 undergoes an alternative splicing that generates two different products of expression. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. The two forms of Bo17 are expressed in BoHV-4 infected cells, but the spliced form is not active, recombinant expression of only the long or the short form of Bo17, RT-PCR expression analysis
-
the recombinant protein generated by mC2GnT-M cDNA exhibits core 2, core 4, and blood group I enzyme activities with a ratio of 1.00:0.46:1.05
transfection in COS-7 cells
-
transfection into CD99 or mock transfectants
transfection into JKT-1 cells
-
wild-type and mutant enzymes expressed in CHO-K1 cells
-