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2.4.1.109: dolichyl-phosphate-mannose-protein mannosyltransferase

This is an abbreviated version!
For detailed information about dolichyl-phosphate-mannose-protein mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.109

Reaction

dolichyl beta-D-mannosyl phosphate
+
L-seryl-[protein]
=
dolichyl phosphate
+
3-O-(alpha-D-mannosyl)-L-seryl-[protein]

Synonyms

AapmtA, AfPmt1p, AnPmtA, AnPmtB, AnPmtC, dolichol phosphomannose-protein mannosyltransferase, dolichyl phosphate-mannose:protein O-mannosyltransferase, dolichyl-phosphate-mannose--protein mannosyltransferase 1, dolichyl-phosphate-mannose--protein mannosyltransferase 2, dolichyl-phosphate-mannose--protein mannosyltransferase 4, dolichyl-phosphate-mannose-protein mannosyltransferase, dolichyl-phosphate-mannose-protein mannosyltransferase 1, dolichyl-phosphate-mannose-protein mannosyltransferase 2, Drosophila O-mannosyltransferase Rotated Abdomen, Drosophila O-mannosyltransferase Twisted, HpPmt1p, mannosyltransferase, dolichol phosphomannose-protein, More, O-glycoside mannosyltransferase, O-mannosyltransferase, O-mannosyltransferase 1, ogm1, ogm2, ogm4, Oma1, Oma2, Oma4, Omg1, Omg2, Omg4, PMT, PMT1, Pmt1 O-mannosyltransferase, Pmt1A, Pmt1D, Pmt1p, Pmt1p-Pmt2p, PMT2, Pmt2A, Pmt2D, Pmt2p, PMT3, PMT4, Pmt4A, Pmt4D, Pmt4p, PMT5, Pmt5p, Pmt6, PMT7, PmtA, pmtB, pmtC, PMTI, POMT1, POMT2, protein mannosyltransferase, protein O-D-mannosyltransferase, protein O-mannosyl-transferase 1, protein O-mannosyl-transferase 2, protein O-mannosyltransferase, protein O-mannosyltransferase 1, protein O-mannosyltransferase 2, protein O-mannosyltransferase A, protein O-mannosyltransferase Pmt4, protein O-mannosyltransferases 1, protein O-mannosyltransferases 2, protein-O-mannosyltransferase-1, protein-Omannosyltransferase, RT, sPOMT2, tPOmt2, TW, Um05433, Um10749, Um11220

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.109 dolichyl-phosphate-mannose-protein mannosyltransferase

Cloned

Cloned on EC 2.4.1.109 - dolichyl-phosphate-mannose-protein mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromosome mapping of PMT1
cloning and overexpression of PMT2 in yeast strain GFUII-4B, showing no alteration of enzyme activity, and co-overexpression with PMT1 in yeast strain TF1.8, leading to 3fold increase in enzyme activity in vitro, thus PMT1 and 2 function as a complex
-
DNA sequence determination
DNA sequence determination, chromosome mapping
expressed in Escherichia coli OrigamiTM (DE 3) cells
-
expressed in HEK-293T cells
-
expression in Saccharomyces cerevisiae
expression of Pmt4 in a Saccharomyces cerevisiae pmt1pmt4 mutant strain functionally complements the deficient Pmt activity
gene PAS_chr2-1_0212, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha
gene PAS_chr2-1_0256, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha
gene pmt1, recombinant expression of His-tagged full-length wild-type enzyme and of truncated mutant Pmt1DELTA311-902 in Pichia pastoris strain GS115 via transformation by Agrobacterium tumefaciens strain AGL-1, quantitative real-time RT-PCR expression and analysis
PCR amplification, creation of fusion enzymes with replacement cassettes
PCR-amplification of blood derived enzyme gene
PCR-amplification of reference strain open reading frames cloned into standard cloning vectors, expressed in fungus
PCR-amplification to create mutants, full gene transfer via expression vector to Schizosaccharomyces pombe (deficient in pmt) for complementation studies
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PCR-amplification, introduction of replacement cassettes to disrupt genes
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recombinant expression of HA-tagged isozyme Pmt1p. In Saccharomyces cerevisiae, C-terminal fusions with HA-, FLAG-, or GFP-epitopes do not affect PMTs mannosyltransferase activity
transfection of expression plasmids with wild-type and mutant enzyme variants into HEK-293T cells
-