2.4.1.123: inositol 3-alpha-galactosyltransferase
This is an abbreviated version!
For detailed information about inositol 3-alpha-galactosyltransferase, go to the full flat file.
Reaction
Synonyms
BhGolS1, CjGolS, CsGolS1, galactinol synthase, galactinol synthase1, galactosyltransferase, uridine diphosphogalactose-inositol, GAS, GOLS, GolS1, GS, inositol 1-alpha-galactosyltransferase, LcGolS1, LcGolS2, More, OJ1165_F02.103, Os07g0687900, OsGolS1, OsGolS2, TsGOLS2, UDP-D-galactose:inositol galactosyltransferase, UDP-galactose:myo-inositol 1-alpha-D-galactosyltransferase, uridine diphosphogalactose-inositol galactosyltransferase, XvGolS
ECTree
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Purification
Purification on EC 2.4.1.123 - inositol 3-alpha-galactosyltransferase
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partially from leaves, by ammonium sulfate fractionation and anion exchange chromatography
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purification of glutathione S-transferase fusion proteins GTS-AtGolS1, 2 and 3, overexpressed in Escherichia coli
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
the soluble protein is separated by SDS-PAGE to check the molecular weight of the recombinant protein, which is then purified using Glutathione Sepharose TM 4B