2.4.1.128: scopoletin glucosyltransferase

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For detailed information about scopoletin glucosyltransferase, go to the full flat file.

Reaction

Synonyms

71C1, 71C3, glucosyltransferase, uridine diphosphoglucose-scopoletin, N-feruloyltyramine glucosyltransferase, NbUGT73A24, NbUGT73A25, scopoletin UGT, SGTase, TOGT, UDP-glucose:phenylpropanoid glucosyltransferase, UDP-glucose:scopoletin glucosyltransferase, UDPglucose:scopoletin O-beta-D-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.128 scopoletin glucosyltransferase

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Results	in table
1	Activating Compound
3	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
6	General Information
3	Inhibitors
24	kcat/KM [mM/s]
26	KM Value [mM]
1	Localization
2	Metals/Ions
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
9	Organism
3	Pathway
3	pH Optimum
3	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
8	Reference
8	Source Tissue
5	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
1	Subunits
12	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
16	Turnover Number [1/s]

Cloned

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Cloned on EC 2.4.1.128 - scopoletin glucosyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A24 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates

Nicotiana benthamiana

-

759956

DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A25 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates

Nicotiana benthamiana

-

759956

expression in Escherichia coli, transgenic plants over-expressing the enzyme in leaves and in roots

Nicotiana tabacum

-

660144

generation of plants overexpressing the enzyme. Over-expression leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance

Nicotiana tabacum

-

660198

recombinant and mutant glycosyltransferase 71C1 is expressed as fusion protein tagged with a 26 kDa GST protein at the N-terminus in Escherichia coli BL21, production of a chimeric mutant derived from 2 Arabidopsis glucosyltransferases, 71C1 and 71C3, the chimera contains the N-terminal domain of 71C1 and the C-terminal domain of 71C3

Arabidopsis thaliana

O82381, Q9LML7

702478

recombinant and mutant glycosyltransferase 71C3 is expressed as fusion protein tagged with a 26 kDa GST protein at the N-terminus in Escherichia coli BL21, production of a chimeric mutant derived from 2 Arabidopsis glucosyltransferases, 71C1 and 71C3, the chimera contains the N-terminal domain of 71C1 and the C-terminal domain of 71C3

Arabidopsis thaliana

O82381, Q9LML7

702478