2.4.1.132: GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
This is an abbreviated version!
For detailed information about GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.132
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2.4.1.132
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alpha-1,2-mannosyltransferase
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lipid-linked
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alpha-1,3-linked
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mannoses
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beta-glcnac
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oligosaccharide-lipids
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mannans
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pharmacology
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medicine
- 2.4.1.132
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alpha-1,2-mannosyltransferase
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lipid-linked
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alpha-1,3-linked
- mannoses
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beta-glcnac
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oligosaccharide-lipids
- mannans
- pharmacology
- medicine
Reaction
Synonyms
Alg2, Alg2 mannosyltransferase, Alg2 MTase, alpha-1,3-mannosyltransferase, alpha-1,3-ManT, asparagine-linked glycosylation 2, GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, GDP-Man:Man1GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase II, hALG2, mannosyltransferase II, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid II, More, MTase, scAlg2, WfcD
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Cloned
Cloned on EC 2.4.1.132 - GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
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ALG2 gene encodes GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, amino acid sequence
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gene ALG2, cloning from Saccharomyces cerevisiae strain W303a, recombinant expression of wild-type and mutant N-terminal thioredoxin (Trx)-His6-tagged enzymes in Escherichia coli strain DE3
gene ALG2, expression analysis by quantitative RT-PCR, coexpression of Alg2 and Runx2 in COS-7 cells, both localizing to different compartments
gene ALG2, recombinant expression of wild-type and mutat V68G enzymes in HEK-293 cells
gene wfcD, included in the O-antigen repeating unit and O-antigen biosynthesis of Escherichia coli strain O141, genetic structure of the gene cluster, overview. Functional recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
hALG2 encodes an alpha-1,3-mannosyltransferase resulting in Manalpha(1-3)ManGlcNAc2-PP-dolichol
MNN1, MNT2 and MNT3 genes encode alpha-1,3-mannosyltransferases, wild type and mutants carrying single and multiple combinations of the disrupted gene
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overexpression in Escherichia coli. Two Alg2 constructs are expressed and isolated, one with the N-terminal TRX domain and C-terminal His and V5 epitope tags and the other with only an N-terminal His tag