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2.4.1.132: GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.132

Reaction

GDP-alpha-D-mannose
+
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
=
GDP
+
alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Synonyms

Alg2, Alg2 mannosyltransferase, Alg2 MTase, alpha-1,3-mannosyltransferase, alpha-1,3-ManT, asparagine-linked glycosylation 2, GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, GDP-Man:Man1GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase II, hALG2, mannosyltransferase II, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid II, More, MTase, scAlg2, WfcD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.132 GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

Cloned

Cloned on EC 2.4.1.132 - GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ALG2 gene encodes GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, amino acid sequence
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gene ALG2, cloning from Saccharomyces cerevisiae strain W303a, recombinant expression of wild-type and mutant N-terminal thioredoxin (Trx)-His6-tagged enzymes in Escherichia coli strain DE3
gene ALG2, expression analysis by quantitative RT-PCR, coexpression of Alg2 and Runx2 in COS-7 cells, both localizing to different compartments
gene ALG2, recombinant expression of wild-type and mutat V68G enzymes in HEK-293 cells
gene wfcD, included in the O-antigen repeating unit and O-antigen biosynthesis of Escherichia coli strain O141, genetic structure of the gene cluster, overview. Functional recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
hALG2 encodes an alpha-1,3-mannosyltransferase resulting in Manalpha(1-3)ManGlcNAc2-PP-dolichol
MNN1 gene encoding Mnn1p is cloned and sequenced
-
MNN1, MNT2 and MNT3 genes encode alpha-1,3-mannosyltransferases, wild type and mutants carrying single and multiple combinations of the disrupted gene
-
overexpression in Escherichia coli. Two Alg2 constructs are expressed and isolated, one with the N-terminal TRX domain and C-terminal His and V5 epitope tags and the other with only an N-terminal His tag
wild type and mutant that lacks NH2-terminal cytoplasmic tail
-