2.4.1.132: GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
This is an abbreviated version!
For detailed information about GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.132
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2.4.1.132
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alpha-1,2-mannosyltransferase
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lipid-linked
-
alpha-1,3-linked
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mannoses
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beta-glcnac
-
oligosaccharide-lipids
-
mannans
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pharmacology
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medicine
- 2.4.1.132
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alpha-1,2-mannosyltransferase
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lipid-linked
-
alpha-1,3-linked
- mannoses
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beta-glcnac
-
oligosaccharide-lipids
- mannans
- pharmacology
- medicine
Reaction
Synonyms
Alg2, Alg2 mannosyltransferase, Alg2 MTase, alpha-1,3-mannosyltransferase, alpha-1,3-ManT, asparagine-linked glycosylation 2, GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, GDP-Man:Man1GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase II, hALG2, mannosyltransferase II, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid II, More, MTase, scAlg2, WfcD
ECTree
2.4.1.132 GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.4.1.132 - GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-alpha-D-mannose + beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
GDP + alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
hALG2
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol
GDP-alpha-D-mannose + Man-(beta1,4)-Gn-(beta1,4)-Gn-PP-phytanyl
GDP + Man-(alpha1,3)[Man-(alpha1,6)]-Man1Gn2-PPhy
GDP-alpha-D-mannose + Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
-
-
-
?
GDP-alpha-D-mannose + P1-[11- (anthracen-9-ylmethoxy) undecyl]-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate
GDP + ?
GDPmannose + acceptor containing mannose alpha-1,3-linked at the non-reducing terminus
?
-
-
-
-
?
GDPmannose + phytanyl-pyrophosphate-linked cellobiose
?
-
synthetic substrate for recombinant alpha-1,3-mannosyltransferase AceA, product is a trisaccharide
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
GDPmannose + undecaprenyl-pyrophosphate-linked cellobiose
?
-
natural substrate
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Escherichia coli O141
-
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol
in eukaryotes, biosynthesis of N-glycans starts with the assembly of the common core oligosaccharide precursor Glc3Man9 GlcNAc2-PP-Dol, the glycan moiety of which is subsequently transferred onto selected Asn-Xaa-(Ser/Thr) acceptor sites of the nascent polypeptide chain by the oligosaccharyl-transferase complex
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol
the biosynthesis of asparagine-linked glycoproteins utilizes a dolichylpyrophosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. Alg2 carries out an alpha1,3-mannosylation of D-Man-beta-(1-4)-D-GlcNAc-beta-(1-4)-D-GlcNAc-diphosphodolichol, followed by an alpha1,6-mannosylation, to form the first branched pentasaccharide intermediate of the dolichol pathway
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol
Alg2 carries out an alpha1,3-mannosylation of Man-beta1,4-GlcNAc-beta1,4-GlcNAc-diphosphodolichol, followed by an alpha1,6-mannosylation, to form the first branched pentasaccharide intermediate of the dolichol pathway
-
-
?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol
Alg2 is able to catalyze both the addition of the alpa1,3- and alpha1,6-linked mannose residue to Man1GlcNAc2-PP-Dol, forming Man2GlcNAc2-PP-Dol (cf EC 2.4.1.132) and subsequently to Man3GlcNAc2-PP-Dol
-
-
?
GDP-alpha-D-mannose + decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose
GDP + ?
i.e. synthetic acceptor GlcNAc-PP-De
-
-
?
GDP-alpha-D-mannose + decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose
GDP + ?
Escherichia coli O141
i.e. synthetic acceptor GlcNAc-PP-De
-
-
?
GDP-alpha-D-mannose + Man-(beta1,4)-Gn-(beta1,4)-Gn-PP-phytanyl
GDP + Man-(alpha1,3)[Man-(alpha1,6)]-Man1Gn2-PPhy
synthesis of acceptor phytanyl oligosaccharide, Man1Gn2-PPhy, from Gn-(beta1,4)-Gn-PP-phytanyl (Gn2-PPhy) using yeast Alg1. Recombinant scAlg2 transfers 2 Man residues to the beta1,4-Man of the Man1Gn2-PPhy substrate with alpha1,6 and alpha1,3-linkages, yielding Man-(alpha1,3)[Man-(alpha1,6)]-Man1Gn2-PPhy, cf. EC 2.4.1.257
-
-
?
GDP-alpha-D-mannose + Man-(beta1,4)-Gn-(beta1,4)-Gn-PP-phytanyl
GDP + Man-(alpha1,3)[Man-(alpha1,6)]-Man1Gn2-PPhy
synthesis of acceptor phytanyl oligosaccharide, Man1Gn2-PPhy, from Gn-(beta1,4)-Gn-PP-phytanyl (Gn2-PPhy) using yeast Alg1. Recombinant scAlg2 transfers 2 Man residues to the beta1,4-Man of the Man1Gn2-PPhy substrate with alpha1,6 and alpha1,3-linkages, yielding Man-(alpha1,3)[Man-(alpha1,6)]-Man1Gn2-PPhy, cf. EC 2.4.1.257
-
-
?
GDP-alpha-D-mannose + P1-[11- (anthracen-9-ylmethoxy) undecyl]-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate
GDP + ?
i.e. GlcNAc-PP-AnthrU
-
-
?
GDP-alpha-D-mannose + P1-[11- (anthracen-9-ylmethoxy) undecyl]-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate
GDP + ?
Escherichia coli O141
i.e. GlcNAc-PP-AnthrU
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
no acceptor is dolichol-phosphate
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
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involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
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the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
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-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
?
additional information
?
-
-
role in the synthesis of the glucuronoxylomannan of the capsule
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-
?
additional information
?
-
enzyme WfcD is expressed in Escherichia coli strain BL21(DE3), and the enzymatic product is identified by liquid chromatography-mass spectrometry (LC-MS), collision-induced dissociation electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MSn), and glycosidase digestion using the donor substrate GDP-Man and the synthetic acceptor substrate decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose (GlcNAc-PP-De). Analysis of donor substrate specificity of WfcD, overview. WfcD is an alpha-1,3-ManT that catalyzes the Man(alpha1-3)-GlcNAc-R linkage formation. No activity with GlcNAc-GlcNAc-PP-De, Glc-PP-De, UDP-GlcNAc, GlcNAc, and GlcNAc-NP
-
-
-
additional information
?
-
Escherichia coli O141
enzyme WfcD is expressed in Escherichia coli strain BL21(DE3), and the enzymatic product is identified by liquid chromatography-mass spectrometry (LC-MS), collision-induced dissociation electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MSn), and glycosidase digestion using the donor substrate GDP-Man and the synthetic acceptor substrate decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose (GlcNAc-PP-De). Analysis of donor substrate specificity of WfcD, overview. WfcD is an alpha-1,3-ManT that catalyzes the Man(alpha1-3)-GlcNAc-R linkage formation. No activity with GlcNAc-GlcNAc-PP-De, Glc-PP-De, UDP-GlcNAc, GlcNAc, and GlcNAc-NP
-
-
-
additional information
?
-
-
hALG2: early steps of dolichol-linked oligosaccharide biosynthesis
-
-
?
additional information
?
-
hALG2: early steps of dolichol-linked oligosaccharide biosynthesis
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-
?
additional information
?
-
-
Mnn1p has 2 conserved aspartate residues necessary for activity
-
-
?
additional information
?
-
-
Mnn1p family of alpha-1,3-mannosyltransferases is responsible for adding the terminal mannose residues of O-linked oligosaccharides, MNT2 and MNT3 genes in combination with MNN1 have overlapping roles in the addition of the fourth and fifth alpha-1,3-linked mannose residues to form Man4 and Man5 oligosaccharides
-
-
?
additional information
?
-
-
primary and secondary structure of the ALG2 protein
-
-
?
additional information
?
-
-
Mnn1p is required for the complex glycosylation of secreted proteins
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-
?
additional information
?
-
Alg2 shows no activity with D-Man-beta-(1-4)-D-GlcNAc-beta-(1-4)-D-GlcNAc-diphosphodolichol
-
-
?
additional information
?
-
unique bifunctionality of Alg2 during lipid-linked oligosaccharide (LLO) synthesis
-
-
-
additional information
?
-
-
Mnn1p family of alpha-1,3-mannosyltransferases is responsible for adding the terminal mannose residues of O-linked oligosaccharides, MNT2 and MNT3 genes in combination with MNN1 have overlapping roles in the addition of the fourth and fifth alpha-1,3-linked mannose residues to form Man4 and Man5 oligosaccharides
-
-
?
additional information
?
-
-
Mnn1p is required for the complex glycosylation of secreted proteins
-
-
?
additional information
?
-
-
Mnn1p has 2 conserved aspartate residues necessary for activity
-
-
?
additional information
?
-
-
primary and secondary structure of the ALG2 protein
-
-
?
additional information
?
-
unique bifunctionality of Alg2 during lipid-linked oligosaccharide (LLO) synthesis
-
-
-
