2.4.1.133: xylosylprotein 4-beta-galactosyltransferase

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For detailed information about xylosylprotein 4-beta-galactosyltransferase, go to the full flat file.

Reaction

Synonyms

B4GALT7, beta-1,4 galactosyltransferase I, beta-1,4 galactosyltransferase V, beta-1,4-galactosyltransferase, beta-1,4-galactosyltransferase 7, beta-1,4-galactosyltransferase I, beta-1,4-galactosyltransferase-I, beta-1,4-galactosyltransferase-T7, beta-1,4-GalT, beta-1,4-GalT 7, beta-1,4-GalT I, beta-1,4-GalT V, beta-1,4-GalT-I, beta1,4-galactosyltransferase 7, beta1,4-galactosyltransferase I, beta1,4-galactosyltransferase VII, beta1,4-galactosyltransferase-7, beta1,4-GalT, beta1,4-GalT-I, beta1,4-GalT7, beta4-galactosyltransferase 7, beta4Gal-T7, beta4galactosyltransferase I, beta4GalT-7, beta4GalT7, dbeta4GalTI, galactosyltransferase I, galactosyltransferase, uridine diphosphogalactose-xylose, GalT I, GalT-I, hbeta4GalT7, MgGalT7, pNP-xylose-beta-1,4-galactosyltransferase, proteoglycan UDP-galactose:beta-xylose beta 1,4-galactosyltransferase I, SQV-3, UDP-D-galactose:D-xylose galactosyltransferase, UDP-D-galactose:xylose galactosyltransferase, UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase, UDPgalactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase, xylosylprotein 4-beta-galactosyltransferase, xylosylprotein beta1,4-galactosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.133 xylosylprotein 4-beta-galactosyltransferase

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Results	in table
1	Activating Compound
16	AA Sequence
1	Application
1	CAS Registry Number
27	Cloned(Commentary)
4	Crystallization (Commentary)
159	Disease/ Diagnostics
10	Expression
20	General Information
3	General Stability
4	IC50 Value
23	Inhibitors
68	kcat/KM [mM/s]
17	Ki Value [mM]
158	KM Value [mM]
13	Localization
13	Metals/Ions
6	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
45	Organism
6	Pathway
10	PDB ID
7	pH Optimum
2	Posttranslational Modification
65	Protein Variants
18	Purification (Commentary)
3	Reaction
5	Reaction Type
43	Reference
1	Renatured (Commentary)
46	Source Tissue
26	Specific Activity [micromol/min/mg]
1	Storage Stability
84	Substrates and Products (Substrate)
5	Subunits
72	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
66	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.4.1.133 - xylosylprotein 4-beta-galactosyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

enzyme in the presence of manganese and UDP, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.0), 1 M NaCl, 15% (v/v) MPD, and 5% (w/v) polyethylene glycol 6000

Drosophila melanogaster

Q8T3P3

719863

hanging drop vapor diffusion method, using 100 mM imidazole (pH 6.5) and 750 mM sodium acetate or 100 mM HEPES buffer (pH 8.0), 100mM serinol, 1.0 M NaCl, 5% (w/v) PEG6000, and 10% 2,4-methanepentadiol

Drosophila melanogaster

Q9VBZ9

736433

purified recombinant detagged enzyme in complex xylobiose, hanging drop vapor diffusion method, mixing of protein solution containing 10 mg/ml protein, 10 mM MnCl2, 10 mM UDP, and 10 mM xylobiose, with a reservoir solution containing 100mM HEPES, pH 8.0, 100mM serinol, 1.0 M NaCl, 5% PEG 6000, and 10% 2-methyl-2,4-pentanediol, crystals of enzyme mutants D318N and D211N protein in complex with UDP-Gal, by hanging drop vapor diffusion method, using a protein solution containing 10 mg/ml protein, 10 mM MnCl2, and 10 mM UDP-Gal, with the precipitating solution containing 100 mM HEPES, pH 8.0, 1.0 M NaCl, 5% PEG 6000, and 10% 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis

Drosophila melanogaster

Q9VBZ9

736433

purified recombinant detagged wild-type and truncated enzymes, hanging drop vapor diffusion method, mixing of 15 mg/ml protein solution with reservoir solution containing 100 mM imidazole, pH 6.5, 5 mM MnCl2, and 750 mM sodium acetate, 18°C, X-ray diffraction structure determination and analysis, molecular replacement using the Drosophila melanogaster beta4GalT7 crystal structure, PDB ID 3LW6, as a search model. For crystal complexes, tetragonal crystals are soaked in the same pH 6.5 reservoir solution containing 15% 2-methyl-2,4-pentanediol at 18 °C for 20 h followed by a brief soak in 100 mM Tris, pH 8.0, 750 mM sodium acetate, 15% 2-methyl-2,4-pentanediol, 5 mM MnCl2, and 5 mM UDP-Gal at room temperature. The monoclinic crystals are obtained within 3 days using 100 mM Tris, pH 8.5, and 8% PEG 8000 as a reservoir solution by mixing it with 20 mg/ml beta4GalT7DELTA81 in 10 mM MES (pH 6.5), 150 mM NaCl, 5 mM MnCl2, and 5 mM UDP-galactose at 18 °C

Homo sapiens

Q9UBV7

736433