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2.4.1.14: sucrose-phosphate synthase

This is an abbreviated version!
For detailed information about sucrose-phosphate synthase, go to the full flat file.

Word Map on EC 2.4.1.14

Reaction

UDP-alpha-D-glucose
+
D-fructose 6-phosphate
=
UDP
 +
sucrose 6F-phosphate

Synonyms

At5g20280, AtSPS, More, SPS, SPS A, SPS1, SPS11, Sps2, SPS4, SPS6, SPS8, SPSA, SpsB, SPSII, sucrose 6-phosphate synthase, sucrose phosphate synthase, sucrose phosphate synthase 4, sucrose phosphate synthase A, sucrose phosphate synthase B, sucrose phosphate synthetase, sucrose phosphate-uridine diphosphate glucosyltransferase, sucrose-P synthase, sucrose-phosphate synthase B, sucrosephosphate-UDP glucosyltransferase, UDP-glucose-fructose-phosphate glucosyltransferase, UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase, uridine diphosphoglucose-fructose phosphate glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.14 sucrose-phosphate synthase

Purification

Purification on EC 2.4.1.14 - sucrose-phosphate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complete separation from sucrose synthetase
-
DE52 anion exchange cellulose chromatography and His tag affinity column chromatography
-
enzyme becomes extremely unstable during the course of purification
-
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity
-
in presence of 2-mercaptoethanol and a phenol absorbing agent under N2
-
native enzyme partially from plant tissue by ammonium sulfate fractionation and dialysis
native isozymes partially by anion exchange chromatography
partial
partial, at least 3 additional proteins could not be removed
-
partial, two forms of the enzyme identified, enzyme becomes extremely unstable during the course of purification
-
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II
-
recombinant enzyme partially from Nicotiana tabacum leaves
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant isozyme from Saccharomyces cerevisiae strain CY1905 by ammonium sulfate fractionation and two different steps of anion exchange chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) and His8-myc-His8 tagged EGFP-enzyme from insect Sf9 cells by anion exchange and nickel affinity chromatography, followed by gel filtration. Purification of the full-length enzyme without His-tag by ion exchange and hydrophobic interaction chromatography, and gel filtration
transgenic SPS from tobacco and rice
-
two forms of the enzyme identified