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2.4.1.15: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

This is an abbreviated version!
For detailed information about alpha,alpha-trehalose-phosphate synthase (UDP-forming), go to the full flat file.

Word Map on EC 2.4.1.15

Reaction

UDP-alpha-D-glucose
+
D-glucose 6-phosphate
=
UDP
+
alpha,alpha-trehalose 6-phosphate

Synonyms

alpha,alpha-trehalose phosphate synthase (UDP-forming), alpha,alpha-trehalose-phosphate synthase (UDP-forming), AtTPS1, AtTPS6, GbTPS, glucosyltransferase, uridine diphosphoglucose phosphate, OtsA, OtsA1, OtsA2, phosphotrehalose-uridine diphosphate transglucosylase, T-6-P, T6P synthase, Ta1210, TPS, TPS-2, TPS-3, TPS1, TPS2, TPS3, TPS5, TPSP, transglucosylase, trehalose 6-phosphate synthase, trehalose 6-phosphate synthase 1, trehalose 6-phosphate synthetase, trehalose phosphate synthase, trehalose phosphate synthase 5, trehalose phosphate synthetase, trehalose phosphate-uridine diphosphate glucosyltransferase, trehalose-6-P synthase, trehalose-6-phosphate synthase, trehalose-6-phosphate synthase 1, trehalose-6-phosphate synthase/phosphatase, trehalose-6P synthase, trehalose-P synthetase, trehalosephosphate-UDP glucosyl transferase, UDP-glucose:D-glucose-6-phosphate 1-alpha-D-glucosyltransferase, UDPglucose-glucose-phosphate glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.15 alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Crystallization

Crystallization on EC 2.4.1.15 - alpha,alpha-trehalose-phosphate synthase (UDP-forming)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.1 M sodium malonate, pH 8.0, 0.1 M Tris, pH 8.0, and 30% (w/v) polyethylene glycol 1000
enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, and 40% (w/v) polyethylene glycol 400
apoenzyme, sitting drop vapor diffusion method, using 0.1 M Tris, pH 8.5, and 25% (w/v) PEG3350
in complex with UDP and D-glucose 6-phosphate, using 14% (w/v) PEG 4000, 0.15 M NaOAc, 0.1 M imidazole buffer (pH 7.5), and 200 mM NaCl
purified recombinant enzyme complexed with either substrate UDP-glucose or the inactive substrate analogue UDP-2-deoxy-2-fluoroglucose, hanging drop vapour diffusion method, 100 nl droplets of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl, with either 25 mM UDP-glucose and 50 mM 1-deoxy-glucose 6-phosphate or 25 mM UDP-2-deoxy-2-fluoroglucose and 20 mM glucose 6-phosphate, precipitant solution: 30% w/v PEG 4000, 200 mM ammonium acetate, 100 mM Tris-HCl, pH 8.0, 36 h, 25% ethylene glycol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.0 A resolution
-
apoenzyme, enzyme in complex with UDP-alpha-D-glucose, and enzyme in complex with UDP-alpha-D-glucose and D-glucose 6-phosphate or UDP and alpha,alpha-trehalose 6-phosphate, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, and 20% (w/v) PEG 3350