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E396A
the mutant shows decreased catalytic activity compared to the wild type enzyme
K294A
the mutant shows decreased catalytic activity compared to the wild type enzyme
R22A
the mutant shows decreased catalytic activity compared to the wild type enzyme
R22G
the mutant shows decreased catalytic activity compared to the wild type enzyme
R289A
the mutant shows decreased catalytic activity compared to the wild type enzyme
R327A
the mutant shows decreased catalytic activity compared to the wild type enzyme
W108A
the mutant shows decreased catalytic activity compared to the wild type enzyme
W108S
the mutant shows decreased catalytic activity compared to the wild type enzyme
Y99A
the mutant shows decreased catalytic activity compared to the wild type enzyme
Y99V
the mutant shows decreased catalytic activity compared to the wild type enzyme
D156G
site-directed mutagenesis of a catalytic site residue, catalytically inactive Tps1 mutant variant
H223Y
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catalytically more active than wild-type enzyme
W111S
site-directed mutagenesis of a catalytic site residue
Y102V
site-directed mutagenesis of a catalytic site residue
additional information
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enzyme-deficient mutant embryos develop more slowly than the wild-type, and do not progress past the torpedo stage to cotyledon stage, overview
additional information
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transgenic plants overexpressing the intrinsic enzyme display higher dehydration resistance and a slightly increased trehalose level, but no other phenotype alterations, transgenic seedling are glucose- and abscisic-acid stress insensitive and are larger than wild-type seedlings, overview
additional information
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hemizygous TPS1/tps1 plants are transformed with an ABI3::AtTPS1 cDNA, ABI3 promoter drives expression in developing and germinating seeds but not thereafter, the ABI3::TPS1 complemented tps1 plantlets accumulate sugars and starch, transcripts associated with carbohydrate metabolism are altered and ABA-responsive transcripts are constitutively induced in ABI3::TPS1 tps1 plantlets, phenotypes, overview
additional information
construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview
additional information
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construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview
additional information
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construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview
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additional information
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expression of TPSP in Saccharomyces cerevisiae is able to restore the growth on glucose of both Saccharomyces tps1Delta and tps1Delta tps2Delta mutants as well as the thermotolerance of the tps2Delta mutant
additional information
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overexpression of the enzyme in flies increases their tolerance to anoxia and shortens the recovery time
additional information
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construction of transgenic Oryza sativa plants expressing the catalytically active enzyme as a fusion protein with trehalose-6-phosphate phosphatase of Escherichia coli under control of the ubiquitin promotor from Zea mays, the transgenic plants produce high levels of trehalose in seeds and leaves, carbohydrate profile of seeds, but not of leaves, is altered, plants show no growth inhibition or altered phenotype
additional information
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construction of transgenic Oryza sativa plants expressing the catalytically active enzyme as a fusion protein with trehalose-6-phosphate phosphatase of Escherichia coli under control of the ubiquitin promotor from Zea mays, the transgenic plants produce high levels of trehalose in seeds and leaves, carbohydrate profile of seeds, but not of leaves, is altered, plants show no growth inhibition or altered phenotype, expression in human fibroblasts renders the cells less sensitive to drought compared to wild-type cells
additional information
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activity of T6P synthase and the accumulation of trehalose during ethanol fermentation are significantly improved by overexpression of TPS1, and especially at 38°C cmpared to 30°C, no obvious difference occurs in the activity of T6P synthase and the trehalose concentration for wild-type strain 10151, and transformed strains 10151-URA3 and 10151-pI-RED1, indicating that the expression of URA3 and YAP1 genes, markers of the expression plasmid, does not affect the synthesis of trehalose
additional information
transgenic Solanum tunerosum plants expressing the yeast TPS1 gene show enhanced drought tolerance, 379 genes of known function in potato show at least a 2fold change in expression across genotypes, stress levels or the interaction between these factors, wild-type leaves have twice as many genes with altered expression in response to stress than TPS1 transgenic leaves, the transgenic and wildtype plants show different contents in sugar metabolites in leaves under drought conditions, four transcription factors are uniquely up-regulated in TPS1 transgenic leaves, phenotypes, overview. Microarray transcriptional and metabolic analysis of the transgenic, drought-tolerant potato line
additional information
transgenic Solanum tunerosum plants expressing the yeast TPS1 gene show enhanced drought tolerance, 379 genes of known function in potato show at least a 2fold change in expression across genotypes, stress levels or the interaction between these factors, wild-type leaves have twice as many genes with altered expression in response to stress than TPS1 transgenic leaves, the transgenic and wildtype plants show different contents in sugar metabolites in leaves under drought conditions, four transcription factors are uniquely up-regulated in TPS1 transgenic leaves, phenotypes, overview. Microarray transcriptional and metabolic analysis of the transgenic, drought-tolerant potato line
additional information
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transgenic Solanum tunerosum plants expressing the yeast TPS1 gene show enhanced drought tolerance, 379 genes of known function in potato show at least a 2fold change in expression across genotypes, stress levels or the interaction between these factors, wild-type leaves have twice as many genes with altered expression in response to stress than TPS1 transgenic leaves, the transgenic and wildtype plants show different contents in sugar metabolites in leaves under drought conditions, four transcription factors are uniquely up-regulated in TPS1 transgenic leaves, phenotypes, overview. Microarray transcriptional and metabolic analysis of the transgenic, drought-tolerant potato line
additional information
construction of a tps1DELTA and a tps1-156 deletion mutant
additional information
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construction of a tps1DELTA and a tps1-156 deletion mutant