2.4.1.170: isoflavone 7-O-glucosyltransferase
This is an abbreviated version!
For detailed information about isoflavone 7-O-glucosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.170
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2.4.1.170
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soybean
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isoflavonoids
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cotyledon
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daidzin
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kaempferol
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genistin
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2-hydroxyisoflavanone
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aglycones
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echinata
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glycyrrhiza
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ammonia-lyase
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phytoalexins
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long-distance
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luteolin
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flooding
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bradyrhizobium
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sojae
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pueraria
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cell-suspension
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phytophthora
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plant-microbe
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lobata
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enrei
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naringenin
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glycitin
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cicer
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glucosyltransferases
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arietinum
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drug development
- 2.4.1.170
- soybean
-
isoflavonoids
- cotyledon
- daidzin
- kaempferol
- genistin
- 2-hydroxyisoflavanone
-
aglycones
- echinata
-
glycyrrhiza
-
ammonia-lyase
-
phytoalexins
-
long-distance
- luteolin
-
flooding
-
bradyrhizobium
- sojae
-
pueraria
-
cell-suspension
-
phytophthora
-
plant-microbe
- lobata
-
enrei
- naringenin
- glycitin
-
cicer
-
glucosyltransferases
- arietinum
- drug development
Reaction
Synonyms
AtGT-2, F7GT, glucosyltransferase, uridine diphosphoglucose-isoflavone 7-O-, GmIF7GT, GmIF7GT1, GmUGT1, GmUGT3, GmUGT4, GmUGT7, GmUGT9, IF7GT, IFGT, isoflavone-7-O-glucosyltransferase, isoflavone-specific 7-O-glucosyltransferase, More, PlUGT1, UDP-glucose-flavonoid 7-O-glucosyltransferase, UDP-glucose: formononetin 7-O-glucosyltransferase, UDP-glucose:isoflavone 7-O-glucosyltransferase, UDPglucose-flavonoid 7-O-glucosyltransferase, UDPglucose:isoflavone 7-O-glucosyltransferase, UGT4, UGT709A4, UGT73F1, uridine diphosphoglucose-isoflavone 7-O-glucosyltransferase
ECTree
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Engineering
Engineering on EC 2.4.1.170 - isoflavone 7-O-glucosyltransferase
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D125A
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in the mutant the kcat is lower and the Km is higher than in wild type enzyme
E376A
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in the mutant the kcat is lower and the Km is higher than in wild type enzyme
E392A
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in the mutant the kcat is lower and the Km is higher than in wild type enzyme
E392D
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in the mutant the kcat is higher and the Km is lower than in wild type enzyme
E456A
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in the mutant the kcat is lower and the Km is slightly lower than in wild type enzyme
H15A
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in the mutant the kcat is lower and the Km is higher than in wild type enzyme
H359A
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in the mutant the kcat is lower and the Km has the same level as in wild type enzyme
H368A
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in the mutant the kcat is higher and the Km is higher than in wild type enzyme
additional information
cell engineering by GmIF7GT enzyme coexpression with acyl-CoA carboxylase alpha and beta subunits, biotin ligase, and acetyl-CoA synthetase from Nocardia farcinia for subsequent malonylization of the reaction product of GmIF7GT-encoded isoflavone 7-O-glucosyltransferase. The isoflavonoids are glycosylated at position 7 by 7-O-glycosyltranferase and are further malonylated at position 6' of glucose by malonyl-CoA:isoflavone 7-O-glucoside-6'-O-malonyltransferase both from Glycine max, Scale up for production using a fermentor
additional information
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Saccharomyces cerevisiae cells expressing PlUGT1 are not able to glycosylate daidzin and puerarin